Genome-wide transcriptome analysis of Clavibacter michiganensis subsp. michiganensis grown in xylem mimicking medium

A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants

“…This result is consistent with recently carried out transcriptome analyses [20], which also emphasized the role of transport processes for C. michiganensis subsp. michiganensis.…”

“…In these studies, C. michiganensis subsp. michiganensis was grown in planta or in vitro in a minimal medium with 5% tomato homogenate, 10% xylem sap or in a xylem surrogate medium [18,19,20]. In the study presented here, culture supernatants of cells grown in M9 and xylem mimicking medium [20] were studied in a proteomic approach.…”

“…michiganensis was grown at 26 to 28 °C in M9 minimal medium (5 g/L glucose, 15 g/L Na 2 HPO 4 × 12 H 2 O, 3 g/L KH 2 PO 4 , 0,5 g/L NaCl, 1 g/L NH 4 Cl, 0.3 g/L methionine, 11 mg/L CaCl 2 , 240 mg/L MgSO 4 , 500 µg/L thiamine, 500 µg/L nicotinic acid, 0.2 mg/L FeCl 3 × 6 H 2 O, 0.01 mg/L CuCl 2 × 2 H 2 O, 0.01 mg/L Na 2 B 4 O 7 × 10 H 2 O, 0.01 mg/L MnCl 2 × 2 H 2 O, 0.01 mg/L (NH 4 ) 6 Mo 7 × 4 H 2 O, 0.04 mg/L ZnCl 2 ) [21] and xylem mimicking medium XMM (1.168 g/L NaCl, 2.02 g/L KNO 3 , 1.233 g/L MgSO 4 , 0.147 g/L CaCl 2 , 22 mg/L KH 2 PO 4 , 56 mg/L K 2 HPO 4 , 1.5 µg/L FeSO 4 , 2 g/L casamino acids) [20]. For solid media, 15 g/L Bacto-agar (Oxoid, Basinstoke, UK) was added.…”

“…Subsequently, the cultures were incubated at 28 °C until the exponential growth phase was reached (OD 600 approx. 0.5) [20] and further processed as described below.…”

“…Unfortunately, from 3080 detected genes in core genome and plasmids pCM1 and pCM2, 1030 were annotated as hypothetical, making an elucidation of their physiological function difficult [12]. Global studies might help to give at least basic information about expression of these proteins and, in fact, several studies using proteome and transcriptome analyses gave valuable information in this respect [18,19,20]. …”