Genome-Wide Identification of the LexA-Mediated DNA Damage Response in Streptomyces venezuelae

Stratton, Kathryn J; Bush, Matthew J.; Chandra, Govind; Stevenson, Clare E. M.; Findlay, Kim; Schlimpert, Susan

doi:10.1128/jb.00108-22

Cited by 8 publications

(8 citation statements)

References 64 publications

(82 reference statements)

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“…The SOS response is classically associated with the activation of prophages, and has recently been described in S. venezuelae (48). In S. ambofaciens ATCC 23877, transcripts of the key actors of the SOS response such as RecA, LexA, RecN, DnaE, RuvA Holliday junction DNA helicase (48) were not up-regulated in the ‘HT’ condition compared to the ‘MM’ reference ( Table S5 ). This result suggests that the SOS response is not involved in the induction of Samy production in the ‘HT’ condition, or may be so transient and/or only present in part of the cell population that it was not detected by our bulk transcriptome analysis.…”

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 98%

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Characterization of a novel temperate phage facilitatingin vitrodispersal of multicellular bacteria

Jaffal,

Kortebi,

Misson

et al. 2023

Preprint

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Streptomycesare renowned for their prolific production of specialized metabolites with applications in medicine and agriculture. These multicellular bacteria present a sophisticated developmental cycle, and play a key role in soil ecology. Little is known aboutStreptomyces-phage interactions and the impact of phages onStreptomycesphysiology. In this study, we investigated the conditions governing the expression and production of ′Samy ′, a prophage found inStreptomyces ambofaciensATCC 23877. This phage is a siphovirus produced simultaneously with the activation of other mobile genetic elements. We show that Samy production increases bacterial dispersal in stress conditions, facilitating the dissemination of the lineage. Altogether, we unveiled a new property of a bacteriophage infection that it is closely linked to the multicellular community life ofStreptomycesbacteria.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 98%

Characterization of a novel temperate phage facilitatingin vitrodispersal of multicellular bacteria

Jaffal,

Kortebi,

Misson

et al. 2023

Preprint

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“…The TD variant is thus characterized by a massive loss of MGEs. This could result from a severe state of oxidative stress, because oxidative DNA damages are known to favor the excision of MGEs through the induction of the SOS stress response [ 28 , 29 ]. The loss of MGEs may give the TD variant a greater genomic stability than its parental strain.…”

Section: Resultsmentioning

confidence: 99%

“…However, a most important question is what could have been the initial triggering event responsible for the high genetic instability of the TD variant. Because the excision of MGE is known to be provoked by oxidative stress [ 28 , 29 ], it might be the deletion of the arseno polyketide pathway that would be responsible for the severe raise in oxidative stress leading to high genetic instability. This genetic instability would have selected events promoting the storage of acetyl-CoA as lipids to reduce the activation of the oxidative metabolism and thus the generation of oxidative stress [ 4 , 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Genome Analysis of a Variant of Streptomyces coelicolor M145 with High Lipid Content and Poor Ability to Synthetize Antibiotics

et al. 2023

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Streptomyces coelicolor M145 is a model strain extensively studied to elucidate the regulation of antibiotic biosynthesis in Streptomyces species. This strain abundantly produces the blue polyketide antibiotic, actinorhodin (ACT), and has a low lipid content. In a process designed to delete the gene encoding the isocitrate lyase (sco0982) of the glyoxylate cycle, an unexpected variant of S. coelicolor was obtained besides bona fide sco0982 deletion mutants. This variant produces 7- to 15-fold less ACT and has a 3-fold higher triacylglycerol and phosphatidylethanolamine content than the original strain. The genome of this variant was sequenced and revealed that 704 genes were deleted (9% of total number of genes) through deletions of various sizes accompanied by the massive loss of mobile genetic elements. Some deletions include genes whose absence could be related to the high total lipid content of this variant such as those encoding enzymes of the TCA and glyoxylate cycles, enzymes involved in nitrogen assimilation as well as enzymes belonging to some polyketide and possibly trehalose biosynthetic pathways. The characteristics of this deleted variant of S. coelicolor are consistent with the existence of the previously reported negative correlation existing between lipid content and antibiotic production in Streptomyces species.

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“…Unfortunately, they did not provide experimental evidence for this claim. Second, Schlimpert and co-workers used a lexA deletion mutant of Streptomyces venezuelae to determine the genes under the control of the SOS response (Stratton et al, 2022 ). Interestingly, they found that secondary metabolism genes were among the SOS-regulon, in addition to the expected DNA damage repair genes.…”

Section: Introductionmentioning

confidence: 99%

Small molecule inducers of actinobacteria natural product biosynthesis

Alwali,

Parkinson

2023

Journal of Industrial Microbiology and Biotechnology

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Actinobacteria are a large and diverse group of bacteria that are known to produce a wide range of secondary metabolites, many of which have important biological activities, including antibiotics, anti-cancer agents, and immunosuppressants. The biosynthesis of these compounds is often highly regulated with many natural products being produced at very low levels in laboratory settings. Environmental factors, such as small molecule elicitors, can induce the production of secondary metabolites. Specifically, they can increase titers of known natural products as well as enabling discovery of novel natural products typically produced at undetectable levels. These elicitors can be natural products, including antibiotics or hormones, or synthetic compounds. In recent years, there has been a growing interest in the use of small molecule elicitors to induce the production of secondary metabolites from actinobacteria, especially for the discovery of natural products from “silent” biosynthetic gene clusters. This review aims to highlight classes of molecules that induce secondary metabolite production in actinobacteria and to describe the potential mechanisms of induction.

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Genome-Wide Identification of the LexA-Mediated DNA Damage Response in Streptomyces venezuelae

Abstract: The transcriptional regulator LexA functions as a repressor of the bacterial SOS response, which is induced under DNA-damaging conditions. This results in the expression of genes important for survival and adaptation.

Cited by 8 publications

References 64 publications

Characterization of a novel temperate phage facilitatingin vitrodispersal of multicellular bacteria

Characterization of a novel temperate phage facilitatingin vitrodispersal of multicellular bacteria

Genome Analysis of a Variant of Streptomyces coelicolor M145 with High Lipid Content and Poor Ability to Synthetize Antibiotics

Small molecule inducers of actinobacteria natural product biosynthesis

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