Genome-Wide Identification of Sultr Genes in Malus domestica and Low Sulfur-Induced MhSultr3;1a to Increase Cysteine-Improving Growth

Xun, Mi; Shi, Juan; Shi, Junyuan; Li, Jiaqi; Shi, Yujia; Yan, Jun; Zhang, Weiwei; Yang, Hongqiang

doi:10.3389/fpls.2021.748242

Cited by 9 publications

(6 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the important role of sulfate in plants, SULTRs in several plant species have been studied. For instance, genomes of higher plants like Arabidopsis thaliana, and rice (12 SULTRs), wheat (11 SULTRs), sorghum (10 SULTRs), and apple (9 SULTRs) have been identified [11][12][13][14]. In this study, we identified and characterized 36 and 45 putative SULTR genes in the genome of C. sativa, and B. napus, respectively (Table S1).…”

Section: Discussionmentioning

confidence: 97%

“…SULTRs are characterized by 12 transmembrane domains (TMDs) and an AntiSigma factor antagonist (STAS) domain at C-terminus, which is critical for sulfate transporter activity [10]. Genomes of higher plants like Arabidopsis thaliana, rice, wheat, sorghum, and apple were reported to have 12, 12, 11, 10, and 9 SULTR genes, respectively [11][12][13][14]. The SULTR family is well characterized in Arabidopsis, and based on their sequence resemblance, function, and location, the sulfate transporters can be divided into four main groups.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide Characterization of Sulfate Transporter Gene Family in Oilseed Crops: Camelina sativa and Brassica napus

Heidari¹,

Hasanzadeh²,

Faraji³

et al. 2022

Preprint

View full text Add to dashboard Cite

Sulfate transporters (SULTRs) are responsible for the uptake of the sulfate (SO42−) ions in the rhizosphere by the roots and their distribution in plant organs. In this study, SULTR family members in the genome of the two oilseed crops, Camelina sativa, and Brassica napus, were identified and characterized based on their sequence structure, duplication events, phylogenetic relationships, phosphorylation sites, and expression levels. Herein, 36 and 45 putative SULTR genes were recognized from the genome of C. sativa, and B. napus, respectively. SULTR proteins were predicted as basophilic proteins with low hydrophilicity in both studied species. According to phylogenetic relationships, we divided SULTRs into five groups, in which SULTRs 3 showed highest variation. Besides, several duplication events were observed between SULTRs. The first duplication event was predicted approximately five million years ago between three SULTRs 3.1 in C. sativa. Two subunits were indicated in the 3D structure of SULTRs that the active binding sites differed between C. sativa and B. napus. According to available RNA-seq data, SULTRs showed diverse expression in tissues and response to stimuli. SULTRs 3 showed an expression in all tissues. SULTRs 3.1 were more upregulated in response to abiotic stresses in C. sativa, while SULTRs 3.3, and SULTRs 2.1 showed an upregulation in B. napus. Furthermore, SULTRs 3 and SULTRs 4.1 showed an upregulation in response to biotic stresses in B. napus. Based on the distribution of cis-regulatory elements in the promoter region, we speculated that SULTRs might be controlled by phytohormones such as ABA, and MeJA. Therefore, it seems that SULTR genes in C. sativa have been more influenced by evolutionary processes and have acquired more diversity.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Characterization of Sulfate Transporter Gene Family in Oilseed Crops: Camelina sativa and Brassica napus

Heidari¹,

Hasanzadeh²,

Faraji³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Due to the important role of sulfate in plants, the SULTRs in several plant species have been studied. For instance, the genomes of higher plants, such as Arabidopsis thaliana , rice (12 SULTRs ), wheat (11 SULTRs ), sorghum (10 SULTRs ), and apple (9 SULTRs ), have been identified [ 11 , 12 , 13 , 14 ]. In this study, we identified and characterized 36 and 45 putative SULTR genes in the genomes of C. sativa and B. napus , respectively ( Table S1 ).…”

Section: Discussionmentioning

confidence: 99%

“…SULTRs are characterized by 12 transmembrane domains (TMDs) and an anti-sigma factor antagonist (STAS) domain at the C-terminus, which is critical for sulfate transporter activity [ 10 ]. The genomes of higher plants, such as Arabidopsis thaliana , rice, wheat, sorghum, and apple, have been reported to have 12, 12, 11, 10, and 9 SULTR genes, respectively [ 11 , 12 , 13 , 14 ]. The SULTR family has been well characterized in Arabidopsis , and sulfate transporters can be divided into four main groups based on their sequence resemblance, function, and location.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Characterization of the Sulfate Transporter Gene Family in Oilseed Crops: Camelina sativa and Brassica napus

Heidari

Hasanzadeh

Faraji

et al. 2023

Plants

View full text Add to dashboard Cite

Sulfate transporters (SULTRs) are responsible for the uptake of sulfate (SO42−) ions in the rhizosphere by roots and their distribution to plant organs. In this study, SULTR family members in the genomes of two oilseed crops (Camelina sativa and Brassica napus) were identified and characterized based on their sequence structures, duplication events, phylogenetic relationships, phosphorylation sites, and expression levels. In total, 36 and 45 putative SULTR genes were recognized in the genomes of C. sativa and B. napus, respectively. SULTR proteins were predicted to be basophilic proteins with low hydrophilicity in both studied species. According to the observed phylogenetic relationships, we divided the SULTRs into five groups, out of which the SULTR 3 group showed the highest variation. Additionally, several duplication events were observed between the SULTRs. The first duplication event occurred approximately five million years ago between three SULTR 3.1 genes in C. sativa. Furthermore, two subunits were identified in the 3D structures of the SULTRs, which demonstrated that the active binding sites differed between C. sativa and B. napus. According to the available RNA-seq data, the SULTRs showed diverse expression levels in tissues and diverse responses to stimuli. SULTR 3 was expressed in all tissues. SULTR 3.1 was more upregulated in response to abiotic stresses in C. sativa, while SULTR 3.3 and SULTR 2.1 were upregulated in B. napus. Furthermore, SULTR 3 and SULTR 4.1 were upregulated in response to biotic stresses in B. napus. Additionally, the qPCR data showed that the SULTRs in C. sativa were involved in the plant’s response to salinity. Based on the distribution of cis-regulatory elements in the promoter region, we speculated that SULTRs might be controlled by phytohormones, such as ABA and MeJA. Therefore, it seems likely that SULTR genes in C. sativa have been more heavily influenced by evolutionary processes and have acquired further diversity. The results reveal new insights of the structures and functions of SULTRs in oilseed crops. However, further analyses, related to functional studies, are needed to uncover the role of SULTRs in the plants’ development and growth processes, as well as in their response to stimuli.

show abstract

“…The SULTR protein is characterized by a sulfate transport anti-sigma (STAS) domain at the C-terminus and a sulfur transport domain at the N-terminus, complemented by 12 transmembrane domains (TMDs) [6]. This protein has undergone comprehensive characterization across diverse plant species, including Arabidopsis [7], sorghum [8], wheat [9], apple [10], Lathyrus sativus [11], Camellia sinensis [12], barley [13], maize [14], tomato [15], radish [16], and others, wherein the SULTR genes have been successfully cloned. Group I of the SULTR genes functioned as a high-affinity transporter responsible for sulfate absorption, primarily expressed in substantial quantities in the roots [17,18].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of the Sulfate Transporter Gene Family and Its Expression Pattern in Response to Selenium and Abiotic Stress in Walnuts (Juglans regia L.)

Zhang,

Xue,

Liu

et al. 2024

Forests

Self Cite

View full text Add to dashboard Cite

The sulfate transporter (SULTR) is responsible for the transport and uptake of sulfate, which plays an indispensable role in the growth cycle of plants and adaptation to plant stress. However, there are few reports on the response and regulation of SULTR gene family members in walnuts (Juglans regia L.) to sodium selenate, low temperatures, high temperatures, and simulated drought stress. In this study, the whole genome of the SULTR genes family in walnuts was identified and analyzed by the bioinformatics method. The results show that the walnut genome contains seventeen JrSULTR genes, which are unevenly distributed on eight chromosomes and can be divided into four subfamilies. Cis-acting elements that respond to stress and participate in the regulation of plant hormones were found in the promoter sequence of the JrSULTR genes. The analysis of transcriptome data showed that the expression of JrSULTR1.2b was significantly upregulated under sodium selenate treatment, and the results of qRT-PCR analysis were basically consistent with the transcriptome data. The expression of JrSULTR3.1a and JrSULTR3.4b increased with the prolongation of simulated drought stress time. The transcription levels of JrSULTR1.2b and JrSULTR3.1a were significantly increased after low-temperature treatment. After 9 h of high-temperature treatment, the expression levels of JrSULTR3.1a and JrSULTR3.3 were significantly increased. JrSULTR1.2b and JrSULTR3.1a showed significant expression specificity under stress treatment. At the same time, we also performed subcellular localization of these two genes, which was consistent with the predicted results and was in the cell membrane, and their regulatory functions need to be further studied. These studies laid the foundation for us to explore the specific function of the JrSULTR genes in alleviating abiotic stress in walnuts.

show abstract

Genome-Wide Identification of Sultr Genes in Malus domestica and Low Sulfur-Induced MhSultr3;1a to Increase Cysteine-Improving Growth

Cited by 9 publications

References 51 publications

Genome-Wide Characterization of Sulfate Transporter Gene Family in Oilseed Crops: Camelina sativa and Brassica napus

Genome-Wide Characterization of Sulfate Transporter Gene Family in Oilseed Crops: Camelina sativa and Brassica napus

Genome-Wide Characterization of the Sulfate Transporter Gene Family in Oilseed Crops: Camelina sativa and Brassica napus

Isolation and Characterization of the Sulfate Transporter Gene Family and Its Expression Pattern in Response to Selenium and Abiotic Stress in Walnuts (Juglans regia L.)

Contact Info

Product

Resources

About