Genome-Wide Identification of GATA Family Genes in Phoebe bournei and Their Transcriptional Analysis under Abiotic Stresses

Abstract

GATA transcription factors are crucial proteins in regulating transcription and are characterized by a type-IV zinc finger DNA-binding domain. They play a significant role in the growth and development of plants. While the GATA family gene has been identified in several plant species, it has not yet been reported in Phoebe bournei. In this study, 22 GATA family genes were identified from the P. bournei genome, and their physicochemical properties, chromosomal distribution, subcellular localization, phylogenetic tree, conserved motif, gene structure, cis-regulatory elements in promoters, and expression in plant tissues were analyzed. Phylogenetic analysis showed that the PbGATAs were clearly divided into four subfamilies. They are unequally distributed across 11 out of 12 chromosomes, except chromosome 9. Promoter cis-elements are mostly involved in environmental stress and hormonal regulation. Further studies showed that PbGATA11 was localized to chloroplasts and expressed in five tissues, including the root bark, root xylem, stem bark, stem xylem, and leaf, which means that PbGATA11 may have a potential role in the regulation of chlorophyll synthesis. Finally, the expression profiles of four representative genes, PbGATA5, PbGATA12, PbGATA16, and PbGATA22, under drought, salinity, and temperature stress, were detected by qRT-PCR. The results showed that PbGATA5, PbGATA22, and PbGATA16 were significantly expressed under drought stress. PbGATA12 and PbGATA22 were significantly expressed after 8 h of low-temperature stress at 10 °C. This study concludes that the growth and development of the PbGATA family gene in P. bournei in coping with adversity stress are crucial. This study provides new ideas for studying the evolution of GATAs, provides useful information for future functional analysis of PbGATA genes, and helps better understand the abiotic stress response of P. bournei.

Morphological and molecular response mechanisms of the root system of different Hemarthria compressa species to submergence stress

“…TFs are central regulators of plant response to abiotic stress ( Sun et al., 2020 ; Yan et al., 2023 ). Many TF families, including bHLH, C2H2, WRKY, MYB, and GATA, have been shown to be involved in plant stress response by activating stress-responsive gene expression ( Ma et al., 2022 ; Yin et al., 2023 ). In this study, many TFs were regulated by submergence stress ( Table 1 ), and these TFs may contribute to the flooding resistance of H. compressa .…”

Genome Identification and Expression Profiling of the PIN-Formed Gene Family in Phoebe bournei under Abiotic Stresses

“…To investigate the response of the PbPIN gene family to abiotic stresses such as drought, salt, and temperature stresses, we focused on five genes (PbPIN1, PbPIN7, PbPIN8, PbPIN9, and PbPIN11) that contained a higher number of elements associated with adversity in their cis-acting elements [22,47]. These five genes were considered to be representative of four different sub-families.…”

“…The hydrophilic region of PIN proteins contains glycosylation and phosphorylation sites, which are closely associated with their functionality and proper localization. [18]; modified from Yin et al [22]). GNO, guanylic acid exchange factor for ADP ribosylation factor; GA, Golgi apparatus; TGN/EE, trans-Golgi network/early endosome.…”

“…This concerted action enhances stress tolerance in plants. [18]; modified from Yin et al [22]). GNO, guanylic acid exchange factor for ADP ribosylation factor; GA, Golgi apparatus; TGN/EE, trans-Golgi network/early endosome.…”

Analysis of the UDP-Glucosyltransferase (UGT) Gene Family and Its Functional Involvement in Drought and Salt Stress Tolerance in Phoebe bournei

“…Previously, we studied transcriptional analysis of the GATA gene family under abiotic stress in Phoebe bournei [ 23 ]. We know that under drought stress, the photosynthesis of P. fortunei is inhibited, the net photosynthetic rate, transpiration rate, and stomatal conductance of its leaves decrease, while the activities of oxidoreductase (POD and SOD) increase, and the ABA content in its roots increases.…”