Genome-wide identification and expression analysis of bHLH transcription factor family in response to cold stress in sweet cherry (Prunus avium L.)

Shen, Tao; Wen, Xiaopeng; Zhuang, Wen; Qiu, Zhilang; Hou, Qiandong; Li, Zhengchun; Mei, Lina; Yu, Haiqin; Qiao, Guo

doi:10.1016/j.scienta.2021.109905

Cited by 36 publications

(18 citation statements)

References 55 publications

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“…As the cold tolerance of trees directly affects their distribution, studying cold tolerance mechanisms could lay the foundation for expanding the range of tree species into colder regions. Several bHLH genes in sweet cherry ( Prunus avium L.), such as PavbHLH1 , PavbHLH18 , PavbHLH28 , PavbHLH60 , PavbHLH61 , PavbHLH65 , and PavbHLH66 , are preliminarily shown to be involved in the response to cold stress based on systematic analyses performed on the genome [ 66 ]. The bHLH gene MdbHLH3 enhances the cold tolerance of apples by regulating the increased accumulation of anthocyanin in low temperatures [ 67 ].…”

Section: Roles Of Bhlh Tfs In Plant Stress Tolerancementioning

confidence: 99%

Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance

Guo

Sun

et al. 2021

IJMS

123

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Named for the characteristic basic helix-loop-helix (bHLH) region in their protein structure, bHLH proteins are a widespread transcription factor class in eukaryotes. bHLHs transcriptionally regulate their target genes by binding to specific positions on their promoters and thereby direct a variety of plant developmental and metabolic processes, such as photomorphogenesis, flowering induction, shade avoidance, and secondary metabolite biosynthesis, which are important for promoting plant tolerance or adaptation to adverse environments. In this review, we discuss the vital roles of bHLHs in plant responses to abiotic stresses, such as drought, salinity, cold, and iron deficiency. We suggest directions for future studies into the roles of bHLH genes in plant and discuss their potential applications in crop breeding.

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Section: Roles Of Bhlh Tfs In Plant Stress Tolerancementioning

confidence: 99%

Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance

Guo

Sun

et al. 2021

IJMS

123

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“…NtbHLH123 transcription factor in tobacco is also a transcriptional activator that controls the expression of genes involved in ROS clearance, which led to the increase in cold tolerance ( Zhao et al, 2018 ). In a study on sweet cherries, 66 bHLH genes were identified and most members of the PavbHLH family are associated with various processes in response to cold stress ( Shen et al, 2021 ). The IbbHLH gene in sweet potato may also play a role in regulating cold stress, and IbbHLH79 was identified as a potential candidate for plant molecular breeding to increase cold tolerance in sweet potatoes ( Jin et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Fine-Tuning Cold Stress Response Through Regulated Cellular Abundance and Mechanistic Actions of Transcription Factors

2022

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Inflictions caused by cold stress can result in disastrous effects on the productivity and survival of plants. Cold stress response in plants requires crosstalk between multiple signaling pathways including cold, heat, and reactive oxygen species (ROS) signaling networks. CBF, MYB, bHLH, and WRKY families are among the TFs that function as key players in the regulation of cold stress response at the molecular level. This review discusses some of the latest understanding on the regulation of expression and the mechanistic actions of plant TFs to address cold stress response. It was shown that the plant response consists of early and late responses as well as memory reprogramming for long-term protection against cold stress. The regulatory network can be differentiated into CBF-dependent and independent pathways involving different sets of TFs. Post-transcriptional regulation by miRNAs, control during ribosomal translation process, and post-translational regulation involving 26S proteosomic degradation are processes that affect the cellular abundance of key regulatory TFs, which is an important aspect of the regulation for cold acclimation. Therefore, fine-tuning of the regulation by TFs for adjusting to the cold stress condition involving the dynamic action of protein kinases, membrane ion channels, adapters, and modifiers is emphasized in this review.

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“…A bHLH domain contains 50–60 amino acids and two functionally distinct regions: the N-terminal basic region, which is involved in the binding to the E-box (CANNTG) DNA sequence, and the HLH domain, which mediates domain dimerization [ 10 , 11 ]. Numerous investigations have shown that bHLH transcription factors play important roles in abiotic stresses, including cold [ 12 ], drought [ 13 ], salinity [ 14 ] and iron deficiency [ 15 ]. Moreover, it has been shown that bHLHs participate in plant defense against pests and pathogens, such as Xanthomonas euvesicatoria [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the Apple (Malus × domestica) MdERF100 in Arabidopsis Increases Resistance to Powdery Mildew

Zhang

Hai

et al. 2021

IJMS

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APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) transcription factors play important roles in plant development and stress response. Although AP2/ERF genes have been extensively investigated in model plants such as Arabidopsis thaliana, little is known about their role in biotic stress response in perennial fruit tree crops such as apple (Malus × domestica). Here, we investigated the role of MdERF100 in powdery mildew resistance in apple. MdERF100 localized to the nucleus but showed no transcriptional activation activity. The heterologous expression of MdERF100 in Arabidopsis not only enhanced powdery mildew resistance but also increased reactive oxygen species (ROS) accumulation and cell death. Furthermore, MdERF100-overexpressing Arabidopsis plants exhibited differential expressions of genes involved in jasmonic acid (JA) and salicylic acid (SA) signaling when infected with the powdery mildew pathogen. Additionally, yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that MdERF100 physically interacts with the basic helix–loop–helix (bHLH) protein MdbHLH92. These results suggest that MdERF100 mediates powdery mildew resistance by regulating the JA and SA signaling pathways, and MdbHLH92 is involved in plant defense against powdery mildew. Overall, this study enhances our understanding of the role of MdERF genes in disease resistance, and provides novel insights into the molecular mechanisms of powdery mildew resistance in apple.

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Genome-wide identification and expression analysis of bHLH transcription factor family in response to cold stress in sweet cherry (Prunus avium L.)

Cited by 36 publications

References 55 publications

Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance

Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance

Fine-Tuning Cold Stress Response Through Regulated Cellular Abundance and Mechanistic Actions of Transcription Factors

Overexpression of the Apple (Malus × domestica) MdERF100 in Arabidopsis Increases Resistance to Powdery Mildew

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