Genome-wide identification and characterization of UGT family in pigeonpea (Cajanus cajan) and expression analysis in abiotic stress

Song, Zhihua; Niu, Lili; Dong, Biying; Wang, Litao; Dong, Mingzhu; Fan, Xiao-Hong; Jian, Yue; Meng, Dong; Fu, Yujie

doi:10.1007/s00468-019-01833-6

Cited by 19 publications

(10 citation statements)

References 45 publications

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“…These results indicate that overexpression of CcCIPK13 and CcCIPK14 enhances drought resistance in pigeon pea and increased plant survival after drought stress. At the same time, we also selected two 'housekeeping' genes CcCBL5 and CcUGT50 (Song et al, 2019(Song et al, , 2020, which were no significant change before and after drought treatment (Figure S5).…”

Section: Overexpression Of Cccipk13 or Cccipk14 In Hairy Roots Enhanc...mentioning

confidence: 99%

The pigeon pea CcCIPK14‐CcCBL1 pair positively modulates drought tolerance by enhancing flavonoid biosynthesis

et al. 2021

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SUMMARY Calcineurin B‐like (CBL)‐interacting protein kinases (CIPKs) play a central role in Ca2+ signalling and promote drought tolerance in plants. The CIPK gene family in pigeon pea (Cajanus cajan L.), a major food crop affected by drought, has not previously been characterised. Here, we identified 28 CIPK genes in the pigeon pea genome. Five CcCIPK genes were strongly upregulated in roots upon drought treatment and were selected for further characterisation. Overexpression of CcCIPK13 and CcCIPK14 increased survival rates by two‐ to three‐fold relative to controls after 14 days of drought. Furthermore, the three major flavonoids, genistin, genistein and apigenin, were significantly upregulated in the same transgenic plants. Using CcCIPK14 as bait, we performed a yeast two‐hybrid screen and identified six interactors, including CcCBL1. CcCIPK14 exhibited autophosphorylation and phosphorylation of CcCBL1 in vitro. CcCBL1‐overexpressed plants displayed higher survival rates upon drought stress as well as higher expression of flavonoid biosynthetic genes and flavonoid content. CcCIPK14‐overexpressed plants in which CcCBL1 transcript levels were reduced by RNA interference had lower survival rates, which indicated CcCBL1 in the same pathway as CcCIPK14. Together, our results demonstrate a role for the CcCIPK14‐CcCBL1 complex in drought stress tolerance through the regulation of flavonoid biosynthesis in pigeon pea.

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Section: Overexpression Of Cccipk13 or Cccipk14 In Hairy Roots Enhanc...mentioning

confidence: 99%

The pigeon pea CcCIPK14‐CcCBL1 pair positively modulates drought tolerance by enhancing flavonoid biosynthesis

et al. 2021

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“…The phylogenetic distribution of putative and functionally characterized UGTs has thus far been analyzed in a total of 27 plant genera with fully sequenced genomes, including one chlorophycean algae ( Chlamydomonas reinhardtii ), one bryophyte ( Physcomitrella patens ), one lycophyte ( Selaginella moellendorffii ) and 24 angiosperms (Li et al ., ; Yonekura‐Sakakibara and Hanada, ; Barvkar et al ., ; Caputi et al ., ; Schweiger et al ., ; Li et al ., ; Huang et al ., ; Wu et al ., ; Xu et al ., ; Yu et al ., ; He et al ., ; Rehman et al ., ; Cheng et al ., ; Song et al ., ; Sun et al ., ; Figure ; Table ). In the two analyses with the largest datasets, 12 and 16 genera were examined, respectively, and identified groups O and P UGTs that are absent in A. thaliana (Yonekura‐Sakakibara and Hanada, ; Caputi et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Phylogenomic analysis of UDP‐dependent glycosyltransferases provides insights into the evolutionary landscape of glycosylation in plant metabolism

Wilson

Tian

2019

The Plant Journal

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Glycosylated metabolites generated by UDP-dependent glycosyltransferases (UGTs) play critical roles in plant interactions with the environment as well as human and animal nutrition. The evolution of plant UGTs has previously been explored, but with a limited taxon sampling. In this study, 65 fully sequenced plant genomes were analyzed, and stringent criteria for selection of candidate UGTs were applied to ensure a more comprehensive taxon sampling and reliable sequence inclusion. In addition to revealing the overall evolutionary landscape of plant UGTs, the phylogenomic analysis also resolved the phylogenetic association of UGTs from free-sporing plants and gymnosperms, and identified an additional UGT group (group R) in seed plants. Furthermore, lineage-specific expansions and contractions of UGT groups were detected in angiosperms, with the total number of UGTs per genome remaining constant generally. The loss of group Q UGTs in Poales and Brassicales, rather than functional convergence in the group Q containing species, was supported by a gene tree of group Q UGTs sampled from many species, and further corroborated by the absence of group Q homologs on the syntenic chromosomal regions in Arabidopsis thaliana (Brassicales). Branch-site analyses of the group Q UGT gene tree allowed for identification of branches and amino acid sites that experienced episodic positive selection. The positively selected sites are located on the surface of a representative group Q UGT (PgUGT95B2), away from the active site, suggesting their role in protein folding/stability or protein-protein interactions.

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“…Different tissues of plants usually contain different secondary metabolites, and diversified glycosyltransferases also have multiple functions for specific metabolites [ 15 ]. The RNA-seq data from M. albus tissues showed that the UGT s were variably expressed in all the investigated tissues (root, stem, leaf, flower, and seed) ( Supplementary Figure S4 ).…”

Section: Resultsmentioning

confidence: 99%

“…UGTs have been previously identified in various plant species, such as Glycine max [ 13 ], Gossypium hirsutum [ 14 ], Cajanus cajan [ 15 ], Citrus grandis [ 16 ], and Broussonetia papyrifera [ 17 ]. A total of 122 UGTs have been identified from Arabidopsis thaliana , which have been clustered into 14 groups based on their amino acid sequences [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…This plant demonstrates high tolerance in extreme environments, such as drought, cold, and high salinity [ 26 ]. Glycosylation plays an important role in enhancing plant resistance to abiotic stress [ 15 , 27 ]. However, the functional redundancy of UGT genes and complex glycosylation modifications of coumarin make it difficult to functionally characterize all UGT genes of M. albus .…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Analysis of the UDP-Glycosyltransferase Family Reveals Its Roles in Coumarin Biosynthesis and Abiotic Stress in Melilotus albus

Duan

et al. 2021

IJMS

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Coumarins, natural products abundant in Melilotus albus, confer features in response to abiotic stresses, and are mainly present as glycoconjugates. UGTs (UDP-glycosyltransferases) are responsible for glycosylation modification of coumarins. However, information regarding the relationship between coumarin biosynthesis and stress-responsive UGTs remains limited. Here, a total of 189 MaUGT genes were identified from the M. albus genome, which were distributed differentially among its eight chromosomes. According to the phylogenetic relationship, MaUGTs can be classified into 13 major groups. Sixteen MaUGT genes were differentially expressed between genotypes of Ma46 (low coumarin content) and Ma49 (high coumarin content), suggesting that these genes are likely involved in coumarin biosynthesis. About 73.55% and 66.67% of the MaUGT genes were differentially expressed under ABA or abiotic stress in the shoots and roots, respectively. Furthermore, the functions of MaUGT68 and MaUGT186, which were upregulated under stress and potentially involved in coumarin glycosylation, were characterized by heterologous expression in yeast and Escherichia coli. These results extend our knowledge of the UGT gene family along with MaUGT gene functions, and provide valuable findings for future studies on developmental regulation and comprehensive data on UGT genes in M. albus.

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Genome-wide identification and characterization of UGT family in pigeonpea (Cajanus cajan) and expression analysis in abiotic stress

Cited by 19 publications

References 45 publications

The pigeon pea CcCIPK14‐CcCBL1 pair positively modulates drought tolerance by enhancing flavonoid biosynthesis

The pigeon pea CcCIPK14‐CcCBL1 pair positively modulates drought tolerance by enhancing flavonoid biosynthesis

Phylogenomic analysis of UDP‐dependent glycosyltransferases provides insights into the evolutionary landscape of glycosylation in plant metabolism

Genome-Wide Analysis of the UDP-Glycosyltransferase Family Reveals Its Roles in Coumarin Biosynthesis and Abiotic Stress in Melilotus albus

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