Genome-Wide Characterization of DGATs and Their Expression Diversity Analysis in Response to Abiotic Stresses in Brassica napus

Abstract

Triacylglycerol (TAG) is the most important storage lipid for oil plant seeds. Diacylglycerol acyltransferases (DGATs) are a key group of rate-limiting enzymes in the pathway of TAG biosynthesis. In plants, there are three types of DGATs, namely, DGAT1, DGAT2 and DGAT3. Brassica napus, an allotetraploid plant, is one of the most important oil plants in the world. Previous studies of Brassica napus DGATs (BnaDGATs) have mainly focused on BnaDGAT1s. In this study, four DGAT1s, four DGAT2s and two DGAT3s were identified and cloned from B. napus ZS11. The analyses of sequence identity, chromosomal location and collinearity, phylogenetic tree, exon/intron gene structures, conserved domains and motifs, and transmembrane domain (TMD) revealed that BnaDGAT1, BnaDGAT2 and BnaDGAT3 were derived from three different ancestors and shared little similarity in gene and protein structures. Overexpressing BnaDGATs showed that only four BnaDGAT1s can restore TAG synthesis in yeast H1246 and promote the accumulation of fatty acids in yeast H1246 and INVSc1, suggesting that the three BnaDGAT subfamilies had greater differentiation in function. Transcriptional analysis showed that the expression levels of BnaDGAT1s, BnaDGAT2s and BnaDGAT3s were different during plant development and under different stresses. In addition, analysis of fatty acid contents in roots, stems and leaves under abiotic stresses revealed that P starvation can promote the accumulation of fatty acids, but no obvious relationship was shown between the accumulation of fatty acids with the expression of BnaDGATs under P starvation. This study provides an extensive evaluation of BnaDGATs and a useful foundation for dissecting the functions of BnaDGATs in biochemical and physiological processes.

Powdery mildew infection induces a non-canonical route to storage lipid formation at the expense of host thylakoid lipids to fuel its spore production

“…Our employed SIGS methodology specifically silences targeted genes with minimal off targets (Methods; McRae et al 2023). Furthermore, the Arabidopsis DGATs evolved independently, contain distinct functional domains, and share little sequence similarity (Yin et al 2022). In seed oil accumulation, AtDGAT1 and AtPDAT1 play dominant roles.…”

“…The three classes of Arabidopsis DGAT enzymes contain distinct conserved domains and have evolved independently in plants (Yin et al 2022). The least studied class, DGAT3 enzymes, are unique in that they are soluble metalloproteins, with no transmembrane domain, and a thioredoxin-like ferredoxin domain containing a [2Fe-2S] cluster (Aymé et al 2018).…”

Integrated Transcriptomic and Metabolomic Analysis Reveal the Dynamic Process of Bama Hemp Seed Development and the Accumulation Mechanism of α-Linolenic Acid and Linoleic Acid

“…JA biosynthesis is caused by ALA into 13-LOXs ions, followed by the action of AOS and propylene oxide cyclase ( AOC ) to produce JA precursors. After 12-OPDA is reduced by the 12-OPDA reductase ( OPR ), it undergoes three rounds of oxidation to produce 7-epijasmonic acid (Figure A). − The JA content is higher in the early seed development stages (day7 and day14) but significantly decreases in the seed development stages. It is speculated that JA accumulation in the early stage is beneficial for seed enlargement and nutrient accumulation.…”

Genome-Wide Identification, Classification, and Expression Analyses of the CsDGAT Gene Family in Cannabis sativa L. and Their Response to Cold Treatment

“…Overexpressing the peanut-derived AhDGAT2a gene in tobacco seeds impacted the transcription level of endogenous tobacco lipid metabolism genes and enhanced the FA content therein [ 26 ]. When the BnDGAT genes were overexpressed, this was sufficient to restore the synthesis of TAG in yeast H1246 while promoting FA accumulation therein [ 27 ].…”