Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study

Shi-lin, Yao; Wang, Qi; Zhang, Zuzhi; Wu, Jia‐Zhu; Cheng, Nan; Xu, Xiaolong; Xie, G.; Cao, Jinshan

doi:10.1007/s11655-014-1957-5

Cited by 8 publications

(6 citation statements)

References 13 publications

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“…Additionally, some scholars have focused on the relationship between multigene complex diseases and the TCM treatment model based on experimental tests and clinical trials. 58 Therefore, multitarget network analysis may be a new research approach to prove the effectiveness of TCM. However, some limitations still exist.…”

Section: Discussionmentioning

confidence: 99%

Studies on the Mechanism of Gegen Qinlian Decoction in Treating Diabetes Mellitus Based on Network Pharmacology

Deng

Liang

et al. 2021

Natural Product Communications

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Diabetes mellitus (DM) is a chronic disease that is very common and seriously threatens patient health. Gegen Qinlian decoction (GQD) has long been applied clinically, but its mechanism in pharmacology has not been extensively and systematically studied. A GQD protein interaction network and diabetes protein interaction network were constructed based on the methods of system biology. Functional module analysis, Kyoto Encyclopedia of Genes and Genomes pathway analysis, and Gene Ontology (GO) enrichment analysis were carried out on the 2 networks. The hub nodes were filtered by comparative analysis. The topological parameters, interactions, and biological functions of the 2 networks were analyzed in multiple ways. By applying GEO-based external datasets to verify the results of our analysis that the Gene Set Enrichment Analysis (GSEA) displayed metabolic pathways in which hub genes played roles in regulating different expression states. Molecular docking is used to verify the effective components that can be combined with hub nodes. By comparing the 2 networks, 24 hub targets were filtered. There were 7 complex relationships between the networks. The results showed 4 topological parameters of the 24 selected hub targets that were much higher than the median values, suggesting that these hub targets show specific involvement in the network. The hub genes were verified in the GEO database, and these genes were closely related to the biological processes involved in glucose metabolism. Molecular docking results showed that 5,7,2', 6'-tetrahydroxyflavone, magnograndiolide, gancaonin I, isoglycyrol, gancaonin A, worenine, and glyzaglabrin produced the strongest binding effect with 10 hub nodes. This compound–target mode of interaction may be the main mechanism of action of GQD. This study reflected the synergistic characteristics of multiple targets and multiple pathways of traditional Chinese medicine and discussed the mechanism of GQD in the treatment of DM at the molecular pharmacological level.

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Section: Discussionmentioning

confidence: 99%

Studies on the Mechanism of Gegen Qinlian Decoction in Treating Diabetes Mellitus Based on Network Pharmacology

Deng

Liang

et al. 2021

Natural Product Communications

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“…Zhou et al 28 showed further evidence for the genetic basis of this constitution by finding genomic variations in DCDC5 associated with Yang-deficiency through linkage disequilibrium single nucleotide polymorphism gene sequencing on 12 family members and three spouses with Yang-deficiency syndrome. A genome-wide association study on Yang-deficient constitution by Yao et al 29 showed that Yang-deficient constitution had polygenic genetic characteristics, and found that polymorphism of RGS6, mGluR5, GAPDHL19 and IKZF1 genes were related to changes in cyclic AMP and cyclic guanosine monophosphate levels, memory, metabolic energy state and immune function respectively in Yang-deficient cohorts.…”

Section: Discussionmentioning

confidence: 99%

Population-based associations between progression of normal-tension glaucoma and Yang-deficient constitution among Chinese persons

Tang

Chen

et al. 2021

Br J Ophthalmol

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PurposeTo explore the association between constitution types as defined by traditional Chinese medicine (TCM) and risk for normal-tension glaucoma (NTG).DesignPopulation-based cohort study.MethodsPersons were identified in a population cohort aged ≥30 years with NTG, defined as having an untreated mean intraocular pressure measurement ≤21 mm Hg over six separate occasions, with no single reading >24 mm Hg (as in the Collaborative Normal Tension Glaucoma Study). The Body Constitution in Traditional Chinese Medicine Questionnaire was used to assess each participant’s TCM constitution types. The association between various constitutions and visual field progression according to Early Manifest Glaucoma Trial criteria was assessed using Cox regression HR models.ResultsAmong 142 participants (245 eyes), 23 persons (17.6%) and 25 eyes (10.2%) progressed, over a mean (SD) follow-up duration of 3.49 (0.99) years. Progression rates were highest in participants with Yang-deficient constitution (n=19, 13.4%), among whom 7 (36.8%) exhibited worsening fields. After adjusting for sex, age, central corneal thickness, retinal nerve fibre layer thickness and mean deviation on visual field testing, Yang-deficient constitution (HR 4.63, 95% CI 1.77 to 12.1, p=0.002) and higher mean intraocular pressure during follow-up (HR 1.25, 95% CI 1.01 to 1.56, p=0.044) were associated with field progression.ConclusionsYang-deficient constitution and higher intraocular pressure are risk factors for visual field progression in NTG patients. Yang deficiency is characterised by abnormal vasoregulation, and these results may be consistent with prior studies linking NTG progression to Raynaud’s phenomenon and migraine.

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“…[10,17] Individuals with yang-deficiency also exhibit alterations in energy metabolism. [18][19][20] The cold intolerance symptoms of cold syndrome are related to an imbalance of the nerve-endocrine-immune system [13,15,16] and impaired thermogenesis in the mitochondria. [21] Mitochondria are cell powerhouses, producing the majority of adenosine triphosphate (ATP), a high-energy compound, which directly regulates the biological processes.…”

Section: Introductionmentioning

confidence: 99%

“…[ 10 , 17 ] Individuals with yang-deficiency also exhibit alterations in energy metabolism. [ 18 – 20 ] The cold intolerance symptoms of cold syndrome are related to an imbalance of the nerve-endocrine-immune system [ 13 , 15 , 16 ] and impaired thermogenesis in the mitochondria. [ 21 ]…”

Section: Introductionmentioning

confidence: 99%

Association of traditional Chinese medicine body constitution and cold syndrome with leukocyte mitochondrial functions: An observational study

Ma¹,

Li²,

Ji³

et al. 2023

Medicine

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Body constitution in traditional Chinese medicine (TCM) refers to the holistic and relatively durable state of an individual, based on the qi and blood assessment, and TCM syndrome is defined as the theoretical abstraction of disease-symptom profiles. The biological basis as related to mitochondria, which produce most of the cellular energy, has not been well studied. This study aimed to elucidate the association of mitochondrial function with TCM body constitution and cold syndrome. Body constitution and cold syndrome in TCM were assessed using the Constitution in Chinese Medicine Questionnaire (CCMQ). The mitochondrial function of peripheral leukocytes was evaluated based on oxygen consumption rate (OCR) and enzyme activity; OCR reflects mitochondrial activity and the capacity to produce adenosine triphosphate (ATP). Cellular adenosine nucleotides and malondialdehyde levels were determined using high-performance liquid chromatography to assess the potential bioenergetic mechanisms. A total of 283 adults participated in this study. Leukocytes from subjects with a balanced constitution had higher OCRs than those with unbalanced constitutions. Yang deficiency and cold syndrome also demonstrated lower energy metabolism, as indicated by reduced basal metabolic rate and cellular levels of ATP and malondialdehyde. Decreased mitochondrial enzyme activity has been observed in individuals with the cold syndrome. Unbalanced body constitutions in TCM impair mitochondrial function in leukocytes, which may contribute to the high disease susceptibility. Cold syndrome is characterized by reduced mitochondrial mass, which may explain its symptoms of low-energy metabolism and cold intolerance.

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Genome-wide association study on susceptibility genes associated with yang-deficiency constitution: A small sample case-control study

Cited by 8 publications

References 13 publications

Studies on the Mechanism of Gegen Qinlian Decoction in Treating Diabetes Mellitus Based on Network Pharmacology

Studies on the Mechanism of Gegen Qinlian Decoction in Treating Diabetes Mellitus Based on Network Pharmacology

Population-based associations between progression of normal-tension glaucoma and Yang-deficient constitution among Chinese persons

Association of traditional Chinese medicine body constitution and cold syndrome with leukocyte mitochondrial functions: An observational study

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