Genome-wide analysis of lectin receptor-like kinase family from Arabidopsis and rice

Abstract

Lectin receptor-like kinases (LecRLKs) are class of membrane proteins found in higher plants that are involved in diverse functions ranging from plant growth and development to stress tolerance. The basic structure of LecRLK protein comprises of a lectin and a kinase domain, which are interconnected by transmembrane region. Here we have identified LecRLKs from Arabidopsis and rice and studied these proteins on the basis of their expression profile and phylogenies. We were able to identify 32 G-type, 42 L-type and 1 C-type LecRLKs from Arabidopsis and 72 L-type, 100 G-type and 1 C-type LecRLKs from rice on the basis of their annotation and presence of lectin as well kinase domains. The whole family is rather intron-less. We have sub-grouped the gene family on the basis of their phylogram. Although on the basis of sequence the members of each group are closely associated but their functions vary to a great extent. The interacting partners and coexpression data of the genes revealed the importance of gene family in physiology and stress related responses. An in-depth analysis on gene-expression suggested clear demarcation in roles assigned to each gene. To gain additional knowledge about the LecRLK gene family, we searched for previously unreported motifs and checked their importance structurally on the basis of homology modelling. The analysis revealed that the gene family has important roles in diverse functions in plants, both in the developmental stages and in stress conditions. This study thus opens the possibility to explore the roles that LecRLKs might play in life of a plant.

Genome wide analysis ofCyclophilingene family from rice and Arabidopsis and its comparison with yeast

“…54 The above examples emphasize the importance of experimental verification of the in-silico data. 55 In conclusion, the cyclophilin family characterized by the presence of the PPIase domain is highly conserved in rice, Arabidopsis and the yeast genomes. The yeast genome carries orthologs for both rice and Arabidopsis cyclophilin genes.…”

The Receptor-Like Kinase SIT1 Mediates Salt Sensitivity by Activating MAPK3/6 and Regulating Ethylene Homeostasis in Rice

“…Lectin RLKs (LecRLKs), which are characterized by an N-terminal lectin domain resembling carbohydrate binding lectin proteins, represent the second largest subfamily of RLKs, with 75 members in Arabidopsis and 173 members in rice (Vaid et al, 2012). They are believed to be involved in saccharide signaling, and they play roles in self-incompatibility and in plant defenses against pathogens and pests, based on the known functions of lectin proteins in cell recognition.…”

Pea lectin receptor-like kinase functions in salinity adaptation without yield penalty, by alleviating osmotic and ionic stresses and upregulating stress-responsive genes

“…LecRLK is an extensively duplicated family with 173 and 75 members in rice and Arabidopsis, respectively (Vaid et al 2012). LecRLKs are plasma membrane proteins with an extracellular region resembling lectin proteins, followed a single-pass transmembrane region and a kinase domain (Vaid et al 2013).…”

“…LecRLKs are plasma membrane proteins with an extracellular region resembling lectin proteins, followed a single-pass transmembrane region and a kinase domain (Vaid et al 2013). The kinase domain, mediating the downstream signaling, is highly conserved among the whole RLK family while the extracellular lectin domain which is the signal perception/recognition domain, is variable even within the LecRLK family (Vaid et al 2012). The lectin region also differs from the lectin proteins in the fact that the lectin domain of LecRLKs has lost its mono/ oligo-saccharide binding capacity, but probably retains its capacity to bind to hydrophobic compounds (such as hormones) and complex saccharides (Barre et al 2002;Hervé et al 1999).…”