Genome-wide analysis and transcript profiling of PSKR gene family members in Oryza sativa

Abstract

Peptide signalling is an integral part of cell-to-cell communication which helps to relay the information responsible for coordinating cell proliferation and differentiation. Phytosulfokine Receptor (PSKR) is a transmembrane LRR-RLK family protein with a binding site for small signalling peptide, phytosulfokine (PSK). PSK signalling through PSKR promotes normal growth and development and also plays a role in defense responses. Like other RLKs, these PSKRs might have a role in signal transduction pathways related to abiotic stress responses. Genome-wide analysis of phytosulfokine receptor gene family has led to the identification of fifteen putative members in the Oryza sativa genome. The expression analysis of OsPSKR genes done using RNA-seq data, showed that these genes were differentially expressed in different tissues and responded specifically to heat, salt, drought and cold stress. Furthermore, the real-time quantitative PCR for fifteen OsPSKR genes revealed temporally and spatially regulated gene expression corresponding to salinity and drought stress. Our results provide useful information for a better understanding of OsPSKR genes and provide the foundation for additional functional exploration of the rice PSKR gene family in development and stress response.

OsPSKR15, a phytosulfokine receptor from rice enhances abscisic acid response and drought stress tolerance

“…ABA accumulates to a high level during drought stress and modulates many downstream stress responses that define plant growth, productivity, and survival (Kalladan et al 2017). Consistent with our previous study, OsPSKR15 is induced with exogenous ABA treatment (Figure 1A; Nagar et al 2020). The promoter region of OsPSKR15 has ABRE motifs, which are binding sites for various ABA‐responsive and drought‐responsive transcription factors, suggesting that OsPSKR15 might induce abiotic stress tolerance through an ABA‐dependent pathway.…”

“…During drought stress, the ABA level increases, and in turn, it modulates many downstream stress responses. OsPSKR15 is induced by exogenous ABA treatment and drought stress (Figure 1A; Nagar et al 2020). OsPSKR15 is involved in the core ABA signaling through AtPYL9/OsPYL11.…”

“…To study whether OsPSKR15-KD can interact with both PYL9 and OsPYL11 in plant cells, bimolecular fluorescence complementation (BiFC) analysis was performed using a transient (Bai et al 2009;Hong et al 1997). OsPSKR15 was identified as one of the abiotic stress-responsive genes of the PSKR family in rice (Nagar et al 2020). In the present study, OsPSKR15 expression increased upon exposure of rice seedlings to ABA (Figure 1A).…”

“…Recent publications reported that drought‐induced PSK production by PHYTASPASE 2 regulates flower drop in tomato (Reichardt et al 2020) and improves drought tolerance in Arabidopsis (Stührwohldt et al 2021). Nagar et al (2020) showed that some members of the rice PSKRs family are abiotic stress‐inducible and proposed that they might be involved in signal transduction pathways related to abiotic stress responses. Several studies also showed that there is cross‐talk between PSK receptor‐mediated signaling and different hormonal signaling (Mosher et al 2013; Sauter, 2015b; Wu et al 2015; Zhang et al 2018a).…”

Controlling the Heterodimerisation of the Phytosulfokine Receptor 1 (PSKR1) via Island Loop Modulation

“…PSK is matured by the proteolytic cleavage of its precursors and then goes through a post-translational sulfanation of its tyrosine residues [ 11 ]. Its main binding partners are the phytosulfokine receptors (PSKR) [ 12 , 13 ]. These receptors were first identified on Daucus carrota , and its gene is conserved among different plants species [ 7 , 10 ].…”