Genome Sequences of Bacteriophages KaiHaiDragon and OneinaGillian, Isolated from Microbacterium foliorum in Riverside, California

Miller, Gillian; Tran, Steven; Abrahams, Rhiannon; Bazan, Daniel; Blaylock, Ethan; Choi, Jessica; Grewal, Shannon; Hernandez, Elmira; Kim, Daniel; Kim, Kay; Lee, Yumin; López, M Carmen; Specht, Rachel; Kalaj, Nancy; Muthiah, Arun; Dean, Natasha; Diaz, Arturo

doi:10.1128/mra.00002-19

Cited by 1 publication

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“…Lytic Siphoviridae phages KaiHaiDragon and OneinaGillian were assigned to Cluster EC and Cluster EG, respectively, based on their genomic structure. Notably, the end of the OneinaGillian genome had an assembly gene order that was highly dissimilar to the rest of the Cluster EG phages [ 9 ]. Phage YuuY was assigned to a small subcluster, EA10, which comprises only four other lytic Siphoviridae bacteriophages.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Phages YuuY, KaiHaiDragon, and OneinaGillian Isolated from Microbacterium foliorum

Kim

Paul

Diaz

2022

IJMS

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Microbacterium foliorum is a Gram-positive bacteria found in organic matter. Three lytic bacteriophages, KaiHaiDragon, OneinaGillian, and YuuY, were isolated from M. foliorum strain NRRL B-24224. Phage YuuY in particular expresses a broad host range as it possesses the ability to infect closely related bacterial species Microbacterium aerolatum at a high plating efficiency. Characterization tests were performed on all three Microbacterium phage to assess morphology, genomic characteristics, pH and thermal stabilities, life cycle, and the type of receptor used for infection. All three phages showed similar pH stability, ranging from pH 5–11, except for KaiHaiDragon, which had a reduced infection effectiveness at a pH of 11. YuuY possessed a significantly higher temperature tolerance compared to the other Microbacterium phages as some phage particles remained viable after incubation temperatures of up to 80 °C. Based on the one-step growth curve assay, all three Microbacterium phages possessed a relatively short latent period of 90 min and an approximately two-fold burst size factor. Moreover, all three phages utilize a carbohydrate receptor to initiate infection. Based on bioinformatics analysis, YuuY, KaiHaiDragon and OneinaGillian were assigned to clusters EA10, EC, and EG, respectively.

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Section: Introductionmentioning

confidence: 99%

Characterization of Phages YuuY, KaiHaiDragon, and OneinaGillian Isolated from Microbacterium foliorum

Kim

Paul

Diaz

2022

IJMS

Self Cite

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Genome Sequences of Bacteriophages KaiHaiDragon and OneinaGillian, Isolated from Microbacterium foliorum in Riverside, California

Abstract: KaiHaiDragon and OneinaGillian are two bacteriophages which have been recovered from soil samples using the bacterial host Microbacterium foliorum. Their genome lengths are 52,992 bp and 61,703 bp, with 91 and 104 predicted open reading frames, respectively.

Cited by 1 publication

References 8 publications

Characterization of Phages YuuY, KaiHaiDragon, and OneinaGillian Isolated from Microbacterium foliorum

Characterization of Phages YuuY, KaiHaiDragon, and OneinaGillian Isolated from Microbacterium foliorum

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