Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus

Goudenège, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; LaPointe, Gisèle; Roux, Frédérique Le; Delbarre-Ladrat, Christine

doi:10.1007/s00253-014-6086-8

Cited by 19 publications

(22 citation statements)

References 63 publications

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“…As the proposed 18-gene cluster contains five glycosyltransferases, of which EPS273-H and EPS273-I belonged to mannosyl transferase and are located adjacently in the gene cluster, genes encoding EPS273-H and EPS273-I were deleted together. Deletion of eps273-H and eps273-I together just changed the polysaccharide molecular weight and homogeneity ( Figure 3 A), indicating that deletion of the two adjacent glycosyltransferases might be complemented by other glycosyltransferases present in the gene cluster, which was consistent with the previous report about expolysaccharide HE800 [ 24 ]. To some extent, the presence of five glycosyltransferases within the 18-gene cluster of P. stutzeri 273 may confer its ability to generate diversity in the glycosidic structure, which may increase its adaption to the changes of natural environment.…”

Section: Discussionsupporting

confidence: 91%

“…A polysaccharide gene cluster ( Figure 2 A) consisting of five GTs and 13 other genes were identified, which is similar to the symbiosis exopolysaccharide biosynthetic genes ( syp ) of Vibrio sp. reported previously [ 24 ]. Within the gene cluster, 14 genes appeared to encode proteins involved in biosynthesis, modification or export of polysaccharides ( Figure 2 A).…”

Section: Resultsmentioning

confidence: 53%

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Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus

Zheng

Sha

et al. 2017

Marine Drugs

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Pseudomonas stutzeri 273 is a marine bacterium producing exopolysaccharide 273 (EPS273) with high anti-biofilm activity against P. aeruginosa PAO1. Here, the complete genome of P. stutzeri 273 was sequenced and the genome contained a circular 5.03 Mb chromosome. With extensive analysis of the genome, a genetic locus containing 18 genes was predicted to be involved in the biosynthesis of EPS273. In order to confirm this prediction, two adjacent genes (eps273-H and eps273-I) encoding glycosyltransferases and one gene (eps273-O) encoding tyrosine protein kinase within the genetic locus were deleted and biosynthesis of EPS273 was checked in parallel. The molecular weight profile of EPS purified from the mutant Δeps273-HI was obviously different from that purified from wild-type P. stutzeri 273, while the corresponding EPS was hardly detected from the mutant Δeps273-O, which indicated the involvement of the proposed 18-gene cluster in the biosynthesis of EPS273. Moreover, the mutant Δeps273-HI had the biofilm formed earlier compared with the wild type, and the mutant Δeps273-O almost completely lost the ability of biofilm formation. Therefore, EPS273 might facilitate the biofilm formation for its producing strain P. stutzeri 273 while inhibiting the biofilm formation of P. aeruginosa PAO1. This study can contribute to better understanding of the biosynthesis of EPS273 and disclose the biological function of EPS273 for its producing strain P. stutzeri 273.

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Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 53%

Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus

Zheng

Sha

et al. 2017

Marine Drugs

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“…Little variation in gene regulation or expression, as well as in encoded proteins could result in slight molecular structural variability of the EPS. It has already been shown that the syp gene cluster encoding the biosynthesis of EPS in V. fischeri and V. diabolicus could be widespread among Vibrionaceae [ 70 ].…”

Section: Resultsmentioning

confidence: 99%

Bioprospecting for Exopolysaccharides from Deep-Sea Hydrothermal Vent Bacteria: Relationship between Bacterial Diversity and Chemical Diversity

et al. 2017

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Many bacteria biosynthesize structurally diverse exopolysaccharides (EPS) and excrete them into their surrounding environment. The EPS functional features have found many applications in industries such as cosmetics and pharmaceutics. In particular, some EPS produced by marine bacteria are composed of uronic acids, neutral sugars, and N-acetylhexosamines, and may also bear some functional sulfate groups. This suggests that they can share common structural features with glycosaminoglycans (GAG) like the two EPS (HE800 and GY785) originating from the deep sea. In an attempt to discover new EPS that may be promising candidates as GAG-mimetics, fifty-one marine bacterial strains originating from deep-sea hydrothermal vents were screened. The analysis of the EPS chemical structure in relation to bacterial species showed that Vibrio, Alteromonas, and Pseudoalteromonas strains were the main producers. Moreover, they produced EPS with distinct structural features, which might be useful for targeting marine bacteria that could possibly produce structurally GAG-mimetic EPS.

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“…Its genome (5.04 mbp) was comprised of two chromosomes, the first (3.23 mbp) being larger than the second (1.81 mbp). Its GC content was typical of other V. diabolicus genomes (44.91%) (Goudenege et al 2014 ), except for a 182 kbp island, located on chromosome 2, which had a GC content of 40.8%. Eighty-two percentage of the island consisted of hypothetical genes.…”

Section: Resultsmentioning

confidence: 82%

Occurrence and significance of pathogenicity and fitness islands in environmental vibrios

2018

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Pathogenicity islands (PAIs) are large genomic regions that contain virulence genes, which aid pathogens in establishing infections. While PAIs in clinical strains (strains isolated from a human infection) are well-studied, less is known about the occurrence of PAIs in strains isolated from the environment. In this study we describe three PAIs found in environmental Vibrio vulnificus and Vibrio parahaemolyticus strains, as well as a genomic fitness island found in a Vibrio diabolicus strain. All four islands had markedly different GC profiles than the rest of the genome, indicating that all of these islands were acquired via lateral gene transfer. Genes on the PAIs and fitness island were characterized. The PAI found in V. parahaemolyticus contained the tdh gene, a collagenase gene, and genes involved in the type 3 secretion system II (T3SS2). A V. vulnificus environmental strain contained two PAIs, a small 25 kbp PAI and a larger 143 kbp PAI. Both PAIs contained virulence genes. Toxin–antitoxin (TA) genes were found in all three species: on the V. diabolicus fitness island, and on the V. parahaemolyticus and V. vulnificus PAIs.

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Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus

Cited by 19 publications

References 63 publications

Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus

Genome Sequence of Pseudomonas stutzeri 273 and Identification of the Exopolysaccharide EPS273 Biosynthesis Locus

Bioprospecting for Exopolysaccharides from Deep-Sea Hydrothermal Vent Bacteria: Relationship between Bacterial Diversity and Chemical Diversity

Occurrence and significance of pathogenicity and fitness islands in environmental vibrios

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