Genome Sequence of the Novel Reassortant Mammalian Orthoreovirus Strain MRV00304/13, Isolated from a Calf with Diarrhea from the United States

Anbalagan, Srivishnupriya; Spaans, Tina; Hause, Ben M.

doi:10.1128/genomea.00451-14

Cited by 17 publications

(21 citation statements)

References 9 publications

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“…However, in the last decade, increasing numbers of studies in humans and other mammals have shown that they can cause severe illness in humans and other mammals, including upper respiratory tract infections, encephalitis, and diarrhea (Chua et al, 2008;Ouattara et al, 2011a;Steyer et al, 2013a). MRVs have been isolated from a broad range of mammalian species, including bats (Hu et al, 2014;Kohl et al, 2012;Lelli et al, 2013;Lorusso et al, 2014;Thalmann et al, 2010;Wang et al, 2015a;Yang et al, 2015), civet cats , cows (Anbalagan et al, 2014), mink (Lian et al, 2013), pigs (Dai et al, 2012b;Kwon et al, 2012b;Thimmasandra Narayanappa et al, 2015;Zhang et al, 2011a), dogs (Decaro et al, 2005) and humans (Chua et al, 2008;Ouattara et al, 2011b). However, porcine MRV failed to arouse the concern of researchers until 2007, when it was first reported as the cause of diarrhea in newborn piglets in China (Zhang et al, 2011a).…”

Section: Introductionmentioning

confidence: 99%

Genetic and pathogenic characterization of a novel reassortant mammalian orthoreovirus 3 (MRV3) from a diarrheic piglet and seroepidemiological survey of MRV3 in diarrheic pigs from east China

Qin

Wang

et al. 2017

Veterinary Microbiology

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Mammalian orthoreoviruses (MRVs), which cause gastrointestinal and respiratory illness, have been isolated from a wide variety of mammalian species including bats, minks, pigs and humans. Here we report the isolation and genetic and pathogenic characterization of a novel MRV type 3 (MRV3), named MRV-ZJ2013, from the diarrheic feces of piglets in Zhejiang province, China. Genomic and phylogenetic analysis shows that MRV-ZJ2013 may have originated from reassortments among mink, bat, and pig MRVs, suggesting the hypothesis that interspecies transmission has occurred in pig herds. Neonatal piglets infected with MRV-ZJ2013 displayed mild clinical signs such as poor appetite and soft feces, but vomiting and diarrhea were not observed. Fecal virus shedding was detected only in three out of six piglets, each for one- or two-day post-infection. In contrast, piglets inoculated with a virulent porcine epidemic diarrhea virus (PEDV) strain as the control group had severe signs characterized by acute vomiting and watery diarrhea. These findings suggest that the virulence of MRV-ZJ2013, if any, was likely not significant compared to that of PEDV. A seroepidemiological survey of MRV by means of an indirect enzyme-linked immune-sorbent assay (ELISA) based on a recombinant MRV3 capsid protein sigma1 as antigen revealed a high seroprevalence (77%) in 1037 samples from diarrheic pigs of different ages from 24 herds in seven provinces of east China between 2015 and 2016, indicating that MRV3 is endemic in pig herds in China, and may contribute collectively to enteric disease along with other porcine pathogens.

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Section: Introductionmentioning

confidence: 99%

Genetic and pathogenic characterization of a novel reassortant mammalian orthoreovirus 3 (MRV3) from a diarrheic piglet and seroepidemiological survey of MRV3 in diarrheic pigs from east China

Qin

Wang

et al. 2017

Veterinary Microbiology

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“…Although MRVs are widely distributed in humans, livestock and wild animals in Asia, Europe and North, and South America [2,16,[19][20][21][22][23][24][25][26][27]29], they have never been detected from pigs in Africa and their epidemiological information in Africa is limited. In this study, we report the isolation and characterization of MRVs circulating in a pig population in Zambia.…”

Section: Discussionmentioning

confidence: 99%

“…MRVs have been identified in humans, livestock and wild animals with a wide host range [2,[19][20][21][22][23][24][25][26]. Individual MRVs can infect multiple species; several interspecies transmission and genetic reassortment events involving both humans and animals have been reported [2,[15][16][17][18][19][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia

Harima

Sasaki

Kajihara

et al. 2020

Journal of General Virology

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Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7 % (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the S3 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs, respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals.

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“…Three large segment (L1, L2, L3), three medium size segments (M1, M2, M3), and four small segments (S1, S2, S3, and S4) Genome encased in a double concentric icosahedral capsid shell (inner shell or core and outer shell) Gene segments are transcribed into full-length mRNAs Gene segments encode one or two proteins: k, l, and r size class proteins Food Environ Virol MRV (Anbalagan et al 2014;Lelli et al 2015). Studies have indicated that 75 % of the RNA (15 9 10 6 Daltons) in the virion is contained in these 10 dsRNA segments, while about 25 % of the RNA in the virions ( *4.5 9 10 6 Daltons) is composed of short single-stranded adenylic-rich oligonucleotides.…”

Section: Organization Of the Reov Particle And Genomementioning

confidence: 99%

Rethinking the Significance of Reovirus in Water and Wastewater

Betancourt

Gerba

2016

Food Environ Virol

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The genus Orthoreovirus contains nonenveloped viruses with double-stranded gene segments encased in a double-layered icosahedral capsid shell. These features constitute major determinants of virion stability in the environment and virion resistance against physical and chemical agents. Reovirus (ReoV) is the general term most commonly used for all virus strains that infect humans and nonhuman animals. Several studies have demonstrated the frequent occurrence of ReoV in wastewaters and natural waters, including surface and ground waters from different geographical areas. Most of these studies have reported higher concentrations of ReoV than any other enteric virus analyzed. They are more commonly isolated in chlorine-disinfected wastewaters than other enteric viruses, and appear to survive longer in water. The ability of ReoV to form large aggregates, even with different types of enteric viruses (e.g., poliovirus) and their ability to undergo mechanisms of gene segment reassortment among different serotypes may also explain their greater stability. Different approaches have been applied for concentration of ReoV from water; however, the recovery efficiency of the filtration methods has not been fully evaluated. Recently, molecular methods for identification of ReoV strains and quantification of virus genome have been developed. Studies have shown that the overall detection sensitivity of ReoV RNA is enhanced through initial replication of infectious virions in cell culture. More studies are needed to specifically address unresolved issues about the fate and distribution of ReoV in the environment since this virus is not commonly included in virological investigations.

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Genome Sequence of the Novel Reassortant Mammalian Orthoreovirus Strain MRV00304/13, Isolated from a Calf with Diarrhea from the United States

Cited by 17 publications

References 9 publications

Genetic and pathogenic characterization of a novel reassortant mammalian orthoreovirus 3 (MRV3) from a diarrheic piglet and seroepidemiological survey of MRV3 in diarrheic pigs from east China

Genetic and pathogenic characterization of a novel reassortant mammalian orthoreovirus 3 (MRV3) from a diarrheic piglet and seroepidemiological survey of MRV3 in diarrheic pigs from east China

Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia

Rethinking the Significance of Reovirus in Water and Wastewater

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