Genome-scale analysis of the metabolic networks of oleaginous Zygomycete fungi

Vongsangnak, Wanwipa; Ruenwai, Rawisara; Tang, Xin; Hu, Xinjie; Zhang, Hao; Shen, Bairong; Song, Yuanda; Laoteng, Kobkul

doi:10.1016/j.gene.2013.03.012

Cited by 37 publications

(27 citation statements)

References 51 publications

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“…Two ME genes coding for a cME and a mME are present in its genome (Vongsangnak et al 2013; Wang et al 2011), and seven distinct isoforms of ME have been reported (Zhang and Ratledge 2008). Homologous over-expression of the cME gene malE1 increased fungal fatty acid content by 30 % without affecting the degree of fatty acid desaturation (Hao et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina

Hao

Chen

et al. 2014

Biotechnol Lett

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Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6) content increased by 60 % without affecting the total fatty acid content. Our results suggest that enhancing mME activity may be an effective mean to increase industrial production of ARA in M. alpina.

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Section: Introductionmentioning

confidence: 99%

Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina

Hao

Chen

et al. 2014

Biotechnol Lett

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“…Unique metabolic features have been identified in Mortierella alpina and Mortierella circinelloides, particularly with respect to NADPH metabolism and sterol biosynthesis, which might be related to differences in fungal lipid phenotype (Vongsangnak et al 2013). The gene coding for acetyl-CoA carboxylase (ACC) was isolated from Mucor rouxii.…”

Section: Filamentous Fungimentioning

confidence: 99%

New Frontiers in the Production of Biodiesel: Biodiesel Derived from Macro and Microorganisms

Leiva-Candia

Dorado

2014

Lecture Notes in Energy

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The biodiesel industry is gaining interest in the past years due to the depletion of the easily extracted petroleum, the increasing demand to the automotive market, and the environmental damage. It is acknowledged that the main obstacle to biodiesel marketing is the cost of production, which is mostly due to the price of the raw material (usually vegetable oils). In this way, the goal is to provide low-cost raw materials. This may be achieved by feedstocks that do not require arable land, do not depend on growing seasons, and that give added value to waste, helping also to its recycling. In this way, oleaginous organisms may be considered an alternative feedstock for the biodiesel industry, as they meet all the previous requirements. This chapter presents the state of the art and the main characteristics of the oil and biodiesel provided by macroorganism (insects) and microorganism (bacteria, filamentous fungi, and yeasts).

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“…Desta maneira, a produção de biodiesel utilizando single cell oil (SCOs) tem recebido importância recentemente por ser uma potencial fonte de energia renovável e compatível com o conceito das biorrefinarias (Vongsangnak et al, 2013). O termo single cell oil foi cunhado com a intenção de denominar principalmente os triglicerídeos de microrganismos, similares aos encontrados em óleos e gorduras provenientes de fontes animais e vegetais.…”

Section: Introductionunclassified

“…Atualmente essa propriedade tem sido muito estudada, uma vez que pode ser extraído facilmente monoésteres da biomassa, o que torna essa espécie uma fonte alternativa de óleo para a produção de biodiesel. Entre os maiores produtores de lipídios encontrase a espécie de fungo Mucor circillenoides que apresenta lipídios ricos em ácidos graxos poliinsaturado, principalmente ácido-γ-linolénico (GLA, 18:3, n-6) de alta demanda comercial, podendo ser utilizado como matéria-prima alternativa na produção de biodiesel (Vongsangnak et al, 2013) .…”

Section: Introductionunclassified

TRANSESTERIFICAÇÃO ENZIMÁTICA DE SINGLE CELL OIL (SCO) DE Mucor circinelloides PARA A PRODUÇÃO DE BIODIESEL

Carvalho¹,

Rivaldi²,

Rós³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -Entre os maiores produtores de lipídios encontra-se o fungo Mucor circillenoides que apresenta lipídios ricos em ácidos graxos poli-insaturados, de alta demanda comercial capazes de atuar como matéria-prima alternativa na produção de biodiesel. Deste modo, o objetivo desse trabalho foi estudar a produção de biodiesel a partir de lipídio produzido por Mucor circinelloides URM 4182. O crescimento celular e a acumulação de lipídios foram investigados em meio contendo glicose como fonte principal de carbono. A extração do lipídio da biomassa foi realizada utilizando etanol como solvente sob irradiação de microondas. O óleo microbiano obtido foi submetido à reação de etanólise utilizando lipase de Candida antarctica (Novozym ® 435) como catalisador. As análises de RMN 1 H e CLAE confirmaram a conversão de 93,2 % do óleo em ésteres de etila e dos teores de mono e diglicerídeos (4,2 e 2,6 % m/m, respectivamente).

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Genome-scale analysis of the metabolic networks of oleaginous Zygomycete fungi

Cited by 37 publications

References 51 publications

Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina

Increased fatty acid unsaturation and production of arachidonic acid by homologous over-expression of the mitochondrial malic enzyme in Mortierella alpina

New Frontiers in the Production of Biodiesel: Biodiesel Derived from Macro and Microorganisms

TRANSESTERIFICAÇÃO ENZIMÁTICA DE SINGLE CELL OIL (SCO) DE Mucor circinelloides PARA A PRODUÇÃO DE BIODIESEL

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