Genome Resource for Peanut Web Blotch Causal Agent<i>Peyronellaea arachidicola</i>Strain YY187

Li, Shaojian; Xue, Xiaonan; Gao, Meng; Wang, Na; Cui, Xiaowei; Sang, Suling; Fan, Wanwan; Wang, Zhenyu

doi:10.1094/pdis-04-20-0898-a

Cited by 9 publications

(9 citation statements)

References 17 publications

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Section: Methodsmentioning

confidence: 99%

“…P. arachidicola YY187 was used to infect peanut plants. This strain was obtained from peanut leaves with symptoms collected in Yuanyang, Henan, China by single spore isolation and maintained in a −80°C freezer by the Crop Protection Laboratory of Henan Academy of Agricultural Science (Li et al, 2021). To produce the inoculum for infection, P. arachidicola mycelium was cultured as a population of spores on oatmeal agar (OA) to induce conidia under alternation of 16 h light and 8 h dark as previously described (Zhang, Zheng, et al, 2019b).…”

Section: Plant and Fungal Materialsmentioning

confidence: 99%

“…Strain YY187 was isolated in Henan province, China. Its draft genome (47.33 Mb) includes 26 contigs, with a G + C content of 56.37% (Li et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Resistance of peanut to web blotch caused by Phoma arachidicola is related to papillae formation and the hypersensitive response

Sun

Cheng

et al. 2022

Plant Pathology

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Peanut (Arachis hypogaea) is an important economic crop that is susceptible to various foliar diseases. Web blotch, which is caused by Phoma arachidicola, is one of the most serious peanut foliar diseases because it affects the leaves and results in substantial yield losses. In this study, the P. arachidicola infection process and the response of peanut plants to infection were revealed through a cytological study of resistant and susceptible peanut varieties. Conidial germination rates, appressorium formation and resistance responses, including papillae formation and the hypersensitive response (HR), were analysed at different infection stages. Notably, the basal infection response of susceptible varieties resulted in limited papillae formation and a single HR, which was followed by programmed cell death. The papillae formation frequency and HR frequency were significantly greater in the resistant varieties than in the susceptible varieties at 84 h postinoculation. The primary difference between the two resistant varieties was the superior HR of Zheng8903 compared with that of Yuhua15. The complex amphidiploid peanut genome may explain the complexity and diversity of the resistance responses. The interactions of functional orthologs should be clarified and perhaps modulated in future investigations. The results of this study revealed the differences in the resistance responses among peanut germplasm and may be relevant for the breeding of new peanut varieties highly resistant to P. arachidicola.

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Section: Methodsmentioning

confidence: 99%

Section: Plant and Fungal Materialsmentioning

confidence: 99%

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Resistance of peanut to web blotch caused by Phoma arachidicola is related to papillae formation and the hypersensitive response

Sun

Cheng

et al. 2022

Plant Pathology

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“…The scaffold N/L 50 of the D. glomerata Pj-2 genome is 11/1,359,674 bp, which is far superior than the other two genome assemblies of D. glomerata strains CBS 528.66 (177 scaffolds; N/L 50 = 7/635,919 bp) and M27-16 (946 scaffolds; N/L 50 = 26/469,072 bp). In addition, the Pj-2 genome assembly is either comparable or better than that of other sequenced Didymella species: D. keratinophila 9M1 (577 scaffolds; N/L 50 = 76/141,571 bp), D. heteroderae 28M1 (620 scaffolds; N/L 50 = 38/283,873 bp), D. pinodes WTN-11-157 (1,593 scaffolds; N/L 50 = 91/106,671 bp), D. lethalis GRM-16-623 (512 scaffolds; N/L50 = 50/196,696), D. arachidicola YY187 (25 scaffolds; N/L 50 = 8/2,070,310 bp) ( Li et al., 2021 ); D. exigua CBS 183.55 (176 scaffolds; N/L 50 = 64/145,421 bp), D. rabiei ArMe14 (34 scaffolds; N/L 50 = 9/1,812,190) ( Shah et al., 2020 ) and D. segeticola GZSQ-4 (23 scaffolds; N/L 50 = 6/2,254,797) ( Ren et al., 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Gapless reference genome assembly of Didymella glomerata, a new fungal pathogen of maize causing Didymella leaf blight

Yang²,

Ding³

et al. 2022

Front. Plant Sci.

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Didymella leaf blight (DLB) caused by Didymella glomerata is a new fungal disease of maize (Zea mays), first detected in 2021 in Panjin, Liaoning province of China. Here we report the reference genome assembly of D. glomerata to unravel how the fungal pathogen controls its virulence on maize at the molecular level. A maize-infecting strain Pj-2 of the pathogen was sequenced on the Illumina NovaSeq 6000 and PacBio Sequel II platforms at a 575-fold genomic coverage. The 33.17 Mb gapless genome assembly comprises 32 scaffolds with L/N50 of 11/1.36 Mb, four of which represent full-length chromosomes. The Pj-2 genome is predicted to contain 10,334 protein-coding genes, of which 211, 12 and 134 encode effector candidates, secondary metabolite backbone-forming enzymes and CAZymes, respectively. Some of these genes are potentially implicated in niche adaptation and expansion, such as colonizing new hosts like maize. Phylogenomic analysis of eight strains of six Didymella spp., including three sequenced strains of D. glomerata, reveals that the maize (Pj-2)- and Chrysanthemum (CBS 528.66)-infecting strains of D. glomerata are genetically similar (sharing 92.37% genome with 98.89% identity), whereas Pj-2 shows truncated collinearity with extensive chromosomal rearrangements with the Malus-infecting strain M27-16 of D. glomerata (sharing only 55.01% genome with 88.20% identity). Pj-2 and CBS 528.66 carry four major reciprocal translocations in their genomes, which may enable them to colonize the different hosts. Furthermore, germplasm screening against Pj-2 led to the identification of three sources of DLB resistance in maize, including a tropical inbred line CML496. DLB resistance in the line is attributed to the accumulation of ROS H2O2 in the apoplastic space of the infected cells, which likely restricts the fungal growth and proliferation.

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“…To date, only two draft genome sequences of D. arachidicola have been reported that referred to genome sizes of 34.11 and 47.30 Mb [ 36 , 37 ]. Notably, in one of the draft genome reports (our previous study), the causal fungus of PWB was misused as Peyronellaea arachidicola [ 37 ], which was reclassified as D. arachidicola in 2020 [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Intraspecific Comparative Analysis Reveals Genomic Variation of Didymella arachidicola and Pathogenicity Factors Potentially Related to Lesion Phenotype

Wang

Gao

et al. 2023

Biology

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Didymella arachidicola is one of the most important fungal pathogens, causing foliar disease and leading to severe yield losses of peanuts (Arachis hypogaea L.) in China. Two main lesion phenotypes of peanut web blotch have been identified as reticulation type (R type) and blotch type (B type). As no satisfactory reference genome is available, the genomic variations and pathogenicity factors of D. arachidicola remain to be revealed. In the present study, we collected 41 D. arachidicola isolates from 26 geographic locations across China (33 for R type and 8 for B type). The chromosome-scale genome of the most virulent isolate (YY187) was assembled as a reference using PacBio and Hi-C technologies. In addition, we re-sequenced 40 isolates from different sampling sites. Genome-wide alignments showed high similarity among the genomic sequences from the 40 isolates, with an average mapping rate of 97.38%. An average of 3242 SNPs and 315 InDels were identified in the genomic variation analysis, which revealed an intraspecific polymorphism in D. arachidicola. The comparative analysis of the most and least virulent isolates generated an integrated gene set containing 512 differential genes. Moreover, 225 genes individually or simultaneously harbored hits in CAZy-base, PHI-base, DFVF, etc. Compared with the R type reference, the differential gene sets from all B type isolates identified 13 shared genes potentially related to lesion phenotype. Our results reveal the intraspecific genomic variation of D. arachidicola isolates and pathogenicity factors potentially related to different lesion phenotypes. This work sets a genomic foundation for understanding the mechanisms behind genomic diversity driving different pathogenic phenotypes of D. arachidicola.

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Genome Resource for Peanut Web Blotch Causal AgentPeyronellaea arachidicolaStrain YY187

Cited by 9 publications

References 17 publications

Resistance of peanut to web blotch caused by Phoma arachidicola is related to papillae formation and the hypersensitive response

Resistance of peanut to web blotch caused by Phoma arachidicola is related to papillae formation and the hypersensitive response

Gapless reference genome assembly of Didymella glomerata, a new fungal pathogen of maize causing Didymella leaf blight

Intraspecific Comparative Analysis Reveals Genomic Variation of Didymella arachidicola and Pathogenicity Factors Potentially Related to Lesion Phenotype

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