Genome Characterization and Probiotic Potential of Corynebacterium amycolatum Human Vaginal Isolates

Abstract

The vaginal microbiome of healthy women contains nondiphtheria corynebacteria. The role and functions of nondiphtheria corynebacteria in the vaginal biotope are still under study. We sequenced and analysed the genomes of three vaginal C. amycolatum strains isolated from healthy women. Previous studies have shown that these strains produced metabolites that significantly increased the antagonistic activity of peroxide-producing lactic acid bacteria against pathogenic and opportunistic microorganisms and had strong antimicrobial activity against opportunistic pathogens. Analysis of the C. amycolatum genomes revealed the genes responsible for adaptation and survival in the vaginal environment, including acid and oxidative stress resistance genes. The genes responsible for the production of H2O2 and the synthesis of secondary metabolites, essential amino acids and vitamins were identified. A cluster of genes encoding the synthesis of bacteriocin was revealed in one of the annotated genomes. The obtained results allow us to consider the studied strains as potential probiotics that are capable of preventing the growth of pathogenic microorganisms and supporting colonisation resistance in the vaginal biotope.

The Urethral Microbiota of Men with and without Idiopathic Urethritis

“… Corynebacterium spp. are considered major constituents of the cutaneous penile microbiota and are also frequently recovered from male urine and urethral samples ( 4 , 9 , 10 , 12 , 19 – 21 ), as well as from vaginal samples ( 19 , 22 ). There are limited data exploring what constitutes optimal genital microbiota in men; however, Corynebacterium are generally considered commensals of the male genital microbiota ( 23 ) and have been associated with positive health outcomes; Corynebacterium pyruviciproducens was negatively associated with urethritis among MSW in one study ( 4 ), and both the presence and abundance of penile Corynebacterium has been associated with optimal vaginal microbiota in female sexual partners ( 20 ).…”

Molecular identification and safety assessment of the potential probiotic strain Bacillus paralicheniformis HMPM220325 isolated from artisanal fruit dairy products

“…These proteins effectively counteract the misfolding and aggregation of proteins that typically occur at elevated temperatures, thereby preserving their structural integrity. 67 The prevention of cold shock in B. paralicheniformis HMPM220325 is facilitated by the presence of cold shock proteins (CspB and CspC) and a DEAD-DEAH box helicase, enabling the strain to sustain its activity at low temperatures. 68 The strain's capacity to withstand temperatures ranging from 20–50 °C was demonstrated through in vitro temperature adaptation experiments.…”

Multi‐omics analysis identifies potential microbial and metabolite diagnostic biomarkers of bacterial vaginosis

“…[38][39][40] The role of non-diphtheriae Corynebacterium in vaginal health, on the other hand, is still in its infancy and was first described by Gladysheva and colleagues. [41][42][43] Cupriavidus spp. have also been reported in vaginal environments infrequently.…”