Genome analysis of the kiwifruit canker pathogen Pseudomonas syringae pv. actinidiae biovar 5

Fujikawa, Takashi; Sawada, Hiroyuki

doi:10.1038/srep21399

Cited by 93 publications

(96 citation statements)

References 19 publications

(58 reference statements)

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“…A number of strategies for canker disease control have been presented (Reglinski et al, 2013); however, none have been shown to be effective in inhibiting Psa from invading into kiwifruit host tissues and cells, and this may be partly due to our limited understanding of its host-pathogen molecular interactions. Based on phylogenetic analysis, Psa strains can be grouped into five biovars (Biovars 1–5; Fujikawa and Sawada, 2016), in which biovar 3 has the high virulence and is the causative agent of the current outbreaks of kiwifruit canker disease. Previous research investigations have revealed that plant recognition receptors proteins (PRRs) genes, resistance (R) genes, and transcriptional factors (TFs) significantly affect plant immune responses to pathogen invasion (Chisholm et al, 2006; Pandey and Somssich, 2009; Kazan and Lyons, 2014).…”

Section: Introductionmentioning

confidence: 99%

Identification of Circular RNAs in Kiwifruit and Their Species-Specific Response to Bacterial Canker Pathogen Invasion

Wang¹,

Liu

et al. 2017

Front. Plant Sci.

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Research studies have recently focused on circle RNAs (circRNAs) in relation to their regulatory functions in animals. However, the systematic identification of circRNAs in plants, especially non-model plants, is limited. In addition, raw report on the prediction of the potential role of circRNAs in plant response to pathogen invasion is currently available. We conducted the systematic identification of circRNAs from four materials originating from three species belonging to genus Actinidia under different situations using ribosomal RNA (rRNA) depleted RNA-Seq data. A total of 3,582 circRNAs were identified in Actinidia, of which 64.01, 21.44, and 14.55% were intergenic circRNAs, exonic circRNAs, and intronic circRNAs, respectively. Tissue-specific expression of circRNAs was observed in kiwifruit, and a species-specific response was detected when infected with Pseudomonas syringae pv. actinidiae (Psa), which is the causative agent of kiwifruit bacterial canker disease. Furthermore, we found that both exonic and intronic circRNAs were significantly positively correlated to parent protein-coding genes, and intronic circRNAs are a class of highly remarkable regulators the parent genes comparing to that of exonic circRNAs. Expression and weighted gene co-expression network analysis (WGCNA) identified a set of circRNAs that were closely associated with plant defense response. The findings of the presents study suggest that circRNAs exhibit tissue- and species-specific expression, as well as play an important role in plant immune response.

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Section: Introductionmentioning

confidence: 99%

Identification of Circular RNAs in Kiwifruit and Their Species-Specific Response to Bacterial Canker Pathogen Invasion

Wang¹,

Liu

et al. 2017

Front. Plant Sci.

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“…Inhibitory effects of the fermentation broth of Actinomycete A217 on pathogenic bacteria were determined using the agar diffusion method (Peela et al ). A 100 ml portion of melted NA (Fujikawa and Sawada ) bacterial culture medium was cooled to 45°C, following which, 10–15 ml of NA was added to the logarithmic growth phase of the tested pathogenic bacterial liquid, which was fully shaken. Each dish (90 mm in diameter) was cultured with 15 ml of carrier medium.…”

Section: Methodsmentioning

confidence: 99%

“…juglandis and Kiwifruit canker caused by Pseudomonas syringae pv. actinidae also cause serious losses to the fruit industry (Lang and Evans ; Fujikawa and Sawada ).…”

Section: Introductionmentioning

confidence: 99%

Identification of antimicrobial metabolites produced by a potential biocontrol Actinomycete strain A217

Hao

Zhou

et al. 2020

J Appl Microbiol

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Aims To extract and identify the metabolites of strain A217 as well as its antifungal spectrum and control effect on various plant pathogens. Methods and Results Strain A217 was identified as a Streptomyces sp. which was most similar to Streptomyces lienomycini. An antimicrobial spectrum test indicated that strain A217 inhibited several plant pathogenic fungi and strong antibacterial effect such as Phytophthora capsici, Botrytis cinerea, Sclerotinia sclerotiorum, Fusarium oxysporum, Pseudomonas syringae and Xanthomonas campestris. An in vivo tissue test demonstrated that the fermentation broth of strain A217 exerted therapeutic and protective effects of 49·47 and 61·60% respectively, on S. sclerotiorum. Additionally, the fermentation broth of A217 exerted control effects on walnut black spot disease in walnut leaves and branches amounting to 79·33 and 81·52% respectively. In a pot experiment, the fermentation broth exhibited a stronger protective and control effect (68·29%), as well as better bacteriostatic and disease control effects on Phytophthora blight of pepper, compared with Metalaxyl. Compounds possessing antifungal and antibacterial activities were obtained from the fermentation broth of strain A217, using column chromatography and HPLC. Chemical and structural analyses conducted using MS and nuclear magnetic resonance confirmed that these compounds were 1H‐pyrrole‐2‐carboxylic acid and 1H‐pyrrole‐2‐carboxamide. The EC50 values of compound 1H‐pyrrole‐2‐carboxylic acid1 for S. sclerotiorum and P. capsici were 20·13 and 50·36 μg ml−1 respectively. Compound 1H‐pyrrole‐2‐carboxamide2 showed significant antibacterial activity against different plant pathogenic bacteria. The MIC values of P. syringae, X. campestris and X. campestris pv. jugiandis were 7·5, 30 and 15·0 μg ml−1 respectively. Conclusions Actinomyces A217 fermentation products have a broad spectrum of bacteriostasis, and have good bacteriostasis activity to many plant pathogenic fungi and bacteria. Significance and Impact of the Study The present study revealed a new antimicrobial producing strain of Streptomyces and its potential application as a biological control agent for plant diseases.

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“…actinidiae biovars and related pathovars. Gene organization of the regions (Genka et al ., ; Sawada et al ., ; Fujikawa & Sawada, , ) is schematically shown. The genes in the homologous relationship are connected by grey boxes.…”

Section: Psa Biovars Found In Japan and Their Characteristicsmentioning

confidence: 99%

Genetic diversity ofPseudomonas syringaepv.actinidiae, pathogen of kiwifruit bacterial canker

Sawada

Fujikawa

2019

Plant Pathology

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Bacterial canker disease of kiwifruit currently occurs in at least 15 countries, causing serious damage. The causative agent of the disease is Pseudomonas syringae pv. actinidiae (Psa), which is genetically diverse and is currently classified into five biovars, namely, biovars 1, 2, 3, 5 and 6. In Japan, four biovars except biovar 2 have been found so far. These biovars have been confirmed to have differences in the virulence and composition of pathogenicity‐related genes, such as toxin biosynthesis and type III effector genes. Biovars 1 and 6 possess the tox island, a genomic island of approximately 38 kb, which contains phaseolotoxin biosynthesis genes (argK‐tox cluster) and is confirmed to have been acquired from other bacteria through horizontal transfers. Also, on the megaplasmid possessed by biovar 6, there exist coronatine biosynthesis genes, and biovar 6 has the ability to produce two phytotoxins, phaseolotoxin and coronatine. In 2014, biovar 3, considered to be of foreign origin, was confirmed for the first time in Japan. Biovar 5, whose virulence is relatively weak, is distributed only in a limited area. In addition to the tox island and various plasmids, a large number of mobile genetic elements are confirmed to be present on the Psa genomes, which might have played a major role in helping Psa to acquire new features. In order to understand how Psa acquired the ability to infect kiwifruit systemically, it is important to make polyphasic comparisons with related pathovars, such as P. syringae pv. theae and pv. actinidifoliorum.

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Genome analysis of the kiwifruit canker pathogen Pseudomonas syringae pv. actinidiae biovar 5

Cited by 93 publications

References 19 publications

Identification of Circular RNAs in Kiwifruit and Their Species-Specific Response to Bacterial Canker Pathogen Invasion

Identification of Circular RNAs in Kiwifruit and Their Species-Specific Response to Bacterial Canker Pathogen Invasion

Identification of antimicrobial metabolites produced by a potential biocontrol Actinomycete strain A217

Genetic diversity ofPseudomonas syringaepv.actinidiae, pathogen of kiwifruit bacterial canker

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