Genistein as a dietary supplement; formulation, analysis and pharmacokinetics study

Mamagkaki, Alexandra; Bouris, Ioannis; Parsonidis, Panagiotis; Vlachou, Ioanna; Gougousi, Maria; Papasotiriou, Ioannis

doi:10.1371/journal.pone.0250599

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…The data revealed low incidences of breast and prostate cancer in the Asian population. This isoflavonoid has been reported to modulate cell cycle and apoptosis, reduced dimethyl-benzanthracene-induced mammary adenocarcinoma and increased cell differentiation 16 . At some higher or lower concentration it has its anticancer effect on ovarian, colon, prostate cancer types as well 17,18 .…”

Section: Discussionmentioning

confidence: 99%

Effect of Genistein on E-Cadherin as Biomarkers in Diagnosis and Treatment of Lung Cancer

Li¹,

Yu²,

Chen³

et al. 2021

International J. of Pharmacology

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Background and Objective: The prevalence of lung cancer is significant enough in recent times and the research is not able to provide availability of treatment for a late-stage cancer patient to increase the survival time and rate. Among many established markers, E-cadherin (involved in cell-cell interactions) has a crucial role to play in subsiding tumour growth and its malignancy. Therefore, the study aims to investigate the effects of Genistein on cell viability on lung cancer cell lines and its expression patterns on E-cadherin and EGFR. Materials and Methods: Lung cancer cell lines were grown and assayed for cell viability for Genistein compared with Cisplatin. The protein samples were further extracted with radio-immunoprecipitation assay and carried forward for western and northern blotting. Furthermore, the effect of Genistein as apoptotic cell death-inducing activity was analyzed using a flow cytometer and estimated with Caspase 3 expression. Results: In the presence of Genistein, cell lines A549, NCI-H820, NCI-H838, NCI-H1155, HCC4006 and H322 showed cell viability less than 40%, at varying concentrations. Genistein at concentration 0.1 µM showed cell viability less than 20% in cell lines NCI-H820, NCI-H838, NCI-H1155, HCC4006 and H322. Further western blot showed the expression of E-cadherin in all cell lines signifying them as biomarkers in NSCLC treatment. Results from flow cytometry also supported the significant effects of Genistein on NSCLC cell lines. Conclusion:The study proposes E-cadherin could be a biomarker for diagnosis of Non-small Cell Lung Cancer (NSCLC) and Genistein remediated up-regulation of E-cadherin could prove to be a novel outcome for the treatment of cancer and prevent malignancy.

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Section: Discussionmentioning

confidence: 99%

Effect of Genistein on E-Cadherin as Biomarkers in Diagnosis and Treatment of Lung Cancer

Li¹,

Yu²,

Chen³

et al. 2021

International J. of Pharmacology

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“…Genistein (4′,5,7-trihydroxyisoflavone) is an isoflavone that is found in soy-based products, being widely used as a supplemental nutrient for years ( Williamson-Hughes et al, 2006 ; Mamagkaki et al, 2021 ). Genistein is also a dual regulator of ROS and calcium ( Uddin and Kabir, 2019 ).…”

Section: Natural Flavones Exhibit Potential In Treating Hf Via Dual Regulation Of Calcium and Rosmentioning

confidence: 99%

Navigating Calcium and Reactive Oxygen Species by Natural Flavones for the Treatment of Heart Failure

Huang

et al. 2021

Front. Pharmacol.

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Heart failure (HF), the leading cause of death among men and women world-wide, causes great health and economic burdens. HF can be triggered by many factors, such as coronary artery disease, heart attack, cardiomyopathy, hypertension, obesity, etc., all of which have close relations with calcium signal and the level of reactive oxygen species (ROS). Calcium is an essential second messenger in signaling pathways, playing a pivotal role in regulating the life and death of cardiomyocytes via the calcium-apoptosis link mediated by the cellular level of calcium. Meanwhile, calcium can also control the rate of energy production in mitochondria that are the major resources of ROS whose overproduction can lead to cell death. More importantly, there are bidirectional interactions between calcium and ROS, and such interactions may have therapeutic implications in treating HF through finely tuning the balance between these two by certain drugs. Many naturally derived products, e.g., flavones and isoflavones, have been shown to possess activities in regulating calcium and ROS simultaneously, thereby leading to a balanced microenvironment in heart tissues to exert therapeutic efficacies in HF. In this mini review, we aimed to provide an updated knowledge of the interplay between calcium and ROS in the development of HF. In addition, we summarized the recent studies (in vitro, in vivo and in clinical trials) using natural isolated flavones and isoflavones in treating HF. Critical challenges are also discussed. The information collected may help to evoke multidisciplinary efforts in developing novel agents for the potential prevention and treatment of HF.

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“…In Pueraria species, the predominant isoflavone is puerarin [ 1 , 4 ]. Isoflavone-rich preparations from soy and red clover and from genistein itself are available as dietary supplements and used against menopause symptoms or to reduce cholesterol levels [ 5 , 6 , 7 ]. Preparations from different Pueraria species are used in Western countries (“Kudzu”) and in traditional medicines in China (“Gegen”) and other Eastern Asian regions for the treatment of cardiovascular diseases, diabetes, liver injury and symptoms of diarrhea and fever, as well as to improve circulation and blood flow [ 8 , 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of α1-Adrenergic, Non-Adrenergic and Neurogenic Human Prostate Smooth Muscle Contraction and of Stromal Cell Growth by the Isoflavones Genistein and Daidzein

Huang

Liu

et al. 2022

Nutrients

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Isoflavone-rich legumes, including soy, are used for food production, as dietary supplements and in traditional medicine. Soy consumption correlates negatively with benign prostatic hyperplasia (BPH) and voiding symptoms. However, isoflavone effects on the prostate are hardly known. Here, we examined the effects on human prostate smooth muscle contractions and stromal cell growth, which are driving factors of voiding symptoms in BPH. Smooth muscle contractions were induced in prostate tissues from radical prostatectomy. Growth-related functions were studied in cultured stromal cells (WPMY-1). Neurogenic, α1-adrenergic and non-adrenergic contractions were strongly inhibited with 50 µM and by around 50% with 10 µM genistein. Daidzein inhibited neurogenic contractions using 10 and 100 µM. Agonist-induced contractions were inhibited by 100 µM but not 10 µM daidzein. A combination of 6 µM genistein with 5 µM daidzein still inhibited neurogenic and agonist-induced contractions. Proliferation of WPMY-1 cells was inhibited by genistein (>50%) and daidzein (<50%). Genistein induced apoptosis and cell death (by seven-fold relative to controls), while daidzein induced cell death (6.4-fold) without apoptosis. Viability was reduced by genistein (maximum: 87%) and daidzein (62%). In conclusion, soy isoflavones exert sustained effects on prostate smooth muscle contractions and stromal cell growth, which may explain the inverse relationships between soy-rich nutrition, BPH and voiding symptoms.

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Genistein as a dietary supplement; formulation, analysis and pharmacokinetics study

Cited by 5 publications

References 29 publications

Effect of Genistein on E-Cadherin as Biomarkers in Diagnosis and Treatment of Lung Cancer

Effect of Genistein on E-Cadherin as Biomarkers in Diagnosis and Treatment of Lung Cancer

Navigating Calcium and Reactive Oxygen Species by Natural Flavones for the Treatment of Heart Failure

Inhibition of α1-Adrenergic, Non-Adrenergic and Neurogenic Human Prostate Smooth Muscle Contraction and of Stromal Cell Growth by the Isoflavones Genistein and Daidzein

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