Genetic variations in the UTR of genes related to molting in Eriocheir sinensis

Abstract

ABSTRACT.Crab grows by periodic molting, which is controlled by moltinhibiting hormone (MIH) and ecdysteroids. Untranslated regions (UTRs) play crucial roles in the posttranscriptional regulation of gene expression. In this study, using crab collected from Changjiang (Yangtze), Huanghe (Yellow), Liaohe, and Yalujiang rivers, 33 haplotypes of the 3ꞌ-UTR of ecdysteroidregulated protein (ERP) gene were identified, of which 14 haplotypes were observed in more than one individual. One hundred and forty clones of haplotype h2 (41.5%) were observed in samples from all the 4 rivers. Three haplotypes were observed to be insertions. For the whole crab sample, we found a positive Tajima's D value and a negative Fu's Fs test (Tajima's D value = 0.98726; Fu's Fs test = -27.382), although the P values were not significant (P > 0.10). The network profile of these 33 haplotypes presented a single core pattern with h2 as the core. In this study, we found that the UTR of ERP gene had a considerably high genetic polymorphism among crab from regions south to north of China. Furthermore, we observed a relatively high genetic divergence among different haplotypes, which would suggest a high diversity of the crab gene pool.

Genetic variation of LEI0258 locus at major histocompatibility complex (MHC) region in Ethiopian indigenous village chicken

“…It has been reported to be associated with chicken performance and disease tolerance (Nikbakht and Esmailnejad, 2015), including allelic variation in antibody responses to vaccination against Newcastle Disease Virus (NDV) (Nikbakht et al, 2013;Baelmans et al, 2005), Marek's disease (Wang et al, 2014;Fulton et al, 2016a), corona virus (Hateren et al, 2013) and coccidiosis, as well as its association with body weight, survival, embryonic mortality, fertilization rate, hatchability, egg production and resistance to worms (Owen et al, 2008). Given its high level of polymorphism and linkage disequilibrium with the MHC-B locus, LEI0258 genotypes have been suggested as an indicator of MHC-B haplotypes, and it has become an important genetic marker used in chicken breed improvement programs (Hoque et al, 2011;Weigend et al, 2001;Banat et ak., 2013;Gao et al, 2015). This has been confirmed by Chazara et al (2013) who has ascertained that the LEI0258 marker genotypes an excellent predictor of the heterozygosity at MHC locus.…”