Genetic Variations at Four Tetrameric Tandem Repeat Loci in Korean Population

Abstract

Allele and genotype frequencies for four tetrameric short tandem repeat (STR) loci, HumFES/FPS, HumFOLP23, HumGABRB15, and HumCYAR04, have been determined by polymerase chain reaction (PCR) amplification and subsequent polyacrylamide gel electrophoresis from approximately 200 genetically unrelated Koreans. This method allows a single base pair resolution and rapid typing with silver staining. The allele and genotype distributions satisfy Hardy-Weinberg expectation. Also, these STR loci have proven to be useful for forensic analyses and paternity tests in which the variable number of tandem repeat (VNTR) loci have some limitations.

Quadruplex amplification of polymorphic STR loci in a Korean population

“…Allele frequency (%) is shown on the Y axis. The numbers under the bars represent core sequence repeats of each loci from expected values, which is consistent with the data of Park et al (1997), even though only 100 samples were tested. This phenomenon was probably due to the historical and genetical homogeneity of the Korean population.…”

“…DNA preparation and amplification were performed using the method described by Park et al (1997). The primer concentrations for each locus were 0.066 µM (D20S470), 0.132 µM (D13S325), 0.132 µM (HumFOLP23) and 0.264 µM (D10S2325).…”

Frequency data on four tetrameric STR loci D18S1270, D14S608, D16S3253 and D21S1437 in a Korean population

“…DNA preparation and amplification were performed as described previously (Park et al 1997;Lee et al 1998). The primer concentrations for each locus were 2.15 µM for D18S1270, 0.3 µM for D14S608, 0.3 µM for D16S3235, and 0.25 µM for D21S1437 ( Table 1).…”

“…The primer concentrations for each locus were 2.15 µM for D18S1270, 0.3 µM for D14S608, 0.3 µM for D16S3235, and 0.25 µM for D21S1437 ( Table 1). The construction of allelic ladders, separation of amplified products and statistical calculations were performed as described previously (Park et al 1997;Lee et al 1998). …”