Genetic Variation of a Lentil (Lens culinaris) Landrace during Three Generations of Breeding

Gleridou, Anthoula; Tokatlidis, Ioannis S.; Polidoros, Alexios N.

doi:10.3390/app12010450

Cited by 4 publications

(2 citation statements)

References 54 publications

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“…This variation helps assess the diversity of a marker/gene/DNA segment in a population which will help in understanding the evolutionary pressure and mutations on the locus over a while. The number of alleles per primer varied from 2 to 5 with an average of 3.60 alleles per primer similar to the results of Gleridou et al, (2022). The sizes of scoring bands ranged from 179 to 244 bp.…”

Section: Molecular Diversitysupporting

confidence: 72%

SSR Marker-based Molecular Characterization of Lentil (Lens culinaris Medik.) Genotypes

Tomar¹,

Sharma²,

Tripathi³

et al. 2023

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Background: Lentil is an important legume crop that plays a vital role in sustainable agriculture and human health. However, the restricted genetic foundation or parentage of contemporary cultivars has arisen as a serious challenge for lentil development. Therefore, determining the genetic diversity and yield-related characteristics is crucial for the breeder to broaden the genetic base and aid in selecting the desirable parents for hybrid development programmes. Methods: In the current study, microsatellite markers were used for diversity analysis among 37 lentil genotypes. Morphological and molecular systems both were able to differentiate lentil genotypes. Among applied SSR primers 10 were able to produce successful amplifications with template DNA of all the studied genotypes of lentil. Result: A sum of 357 scoreable bands were produced, 148 of which accounted for 41.45% polymorphism. The UPGMA dendrogram grouped 37 lentil genotypes into 2 groups. PIC values ranged from 0.37 to 0.77. For this experiment, SSR 80, SSR 130, SSR 34-2 and SSR 33 were highly informative polymorphic markers, demonstrating the efficacy and higher resolution in exposing molecular genetic diversity among lentil genotypes. The highest numbers of alleles (5) were produced by SSR 80 primer which was selected as extremely polymorphic. This study reveals the variation across lentil genotypes, which might be employed further in breeding efforts for lentils that result in strong heterosis in the segregating generation. The SSR markers found as polymorphic may also be utilized for further polymorphism analysis among different set of lentil genotypes.

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Section: Molecular Diversitysupporting

confidence: 72%

SSR Marker-based Molecular Characterization of Lentil (Lens culinaris Medik.) Genotypes

Tomar¹,

Sharma²,

Tripathi³

et al. 2023

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“…Lentil's transparent and thin seed coat is controlled by a recessive gene (tan) which moderates the tannin precursors that are responsible for seed darkening during cooking and the preservation period [9]. It is worth noting that both findings of our research group [10][11][12][13] and those of other research teams (for a review see Polidoros et al [1]) indicate that the genetic diversity present within and among lentil populations is rich enough to provide material for genetic studies and manipulation of this trait. Phenolic acids, stilbenes and flavonoids such as flavanones, flavones, dihydroflavonols, flavonols, flavan-3-ols, anthocyanidins and proanthocyanidins, also known as condensed tannins, are some of the derivatives of polyphenolic compounds that play an important role in the properties of the seed coat [14].…”

Section: Introductionmentioning

confidence: 69%

The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development

Koura,

Pistikoudi,

Tsifintaris

et al. 2023

Applied Sciences

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Seed coat hardness and water permeability, which are determined by the accumulation of tannins through the phenylpropanoid pathway in the seed, are important lentil quality characteristics. The impact of seeds’ developmental stage and phosphorus (P) fertilization levels on tannin accumulation is still under research. Through RNA sequencing, this study explored the effect of three P treatments (P0, 6 mg kg−1; P1, 15 mg kg−1; and P2, 21 mg kg−1) and three seed maturity stages (S1, immature 2 mm seed in a flat pod; S2, fully developed seed within the pod; and S3, mature seed at the beginning of the pod’s discoloration) on lentil gene expression. The key findings highlighted a significant influence of the seed maturity stage on phenylpropanoid genes, with S1 displaying the highest expression levels, and on phosphorus-related Gene Ontology (GO) terms that presented the highest number of downregulated genes in the S3 to S1 comparison. P exhibited a targeted effect on the flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) genes and specific gene clusters, as shown by the differential gene expression analysis. This study investigates the molecular mechanisms related to phosphorus fertilization and seed maturity stages that influence tannin accumulation, offering valuable information for the enhancement of lentil product quality through breeding programs.

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Landrace legislation in the world: status and perspectives with emphasis in EU system

Thanopoulos,

Negri,

Pinheiro de Carvalho

et al. 2024

Genet Resour Crop Evol

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Crop landraces are genetically variable populations of agricultural plant species that through natural evolution and farmers’ selection and continuous cultivation have been adapted to the environment of their origin or cultivation. To be used and officially traded, there are more lax or strict registration schemes throughout the world concerning the application of distinctiveness, uniformity, stability (DUS) system. This review discusses the legislative framework of various countries worldwide and evaluates its application efficiency with a detailed focus on European Union (EU) experience. Especially in EU, landraces must be registered as conservation varieties in the European Catalogue of Varieties. A total of 313 agricultural and 173 vegetable conservation varieties were registered in the European Catalogues from 2013 to 2021. However, it is not clear how many of these registries are landraces because obsolete varieties are also included under the term conservation varieties. Moreover, our review reports the importance of landraces for the FAO (Food and Agriculture Organization of the United Nations) and EU strategies, namely ‘Farm to Fork’ and ‘Biodiversity’. Additionally, the DUS criteria were evaluated for their use when a crop landrace is registered taking into consideration the genetic structure of a landrace. Furthermore, the connection of landraces with Farmers’ Rights, their appropriateness for organic agriculture, and trade issues are discussed. Finally, the new proposal of European Commission on Plant Reproductive Material concerning landraces is critically reviewed and improvements are suggested.

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Genetic Variation of a Lentil (Lens culinaris) Landrace during Three Generations of Breeding

Cited by 4 publications

References 54 publications

SSR Marker-based Molecular Characterization of Lentil (Lens culinaris Medik.) Genotypes

SSR Marker-based Molecular Characterization of Lentil (Lens culinaris Medik.) Genotypes

The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development

Landrace legislation in the world: status and perspectives with emphasis in EU system

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