Genetic Variation in Safflower (Carthamus tinctorious L.) for Seed Quality-Related Traits and Inter-Simple Sequence Repeat (ISSR) Markers

Golkar, Pooran; Arzani, Ahmad; Rezaei, A M

doi:10.3390/ijms12042664

Cited by 70 publications

(45 citation statements)

References 25 publications

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“…Similar results were obtained in the study by Arzani and Rezaei (2011). Similarities in genotypes grouped in the same cluster could also appear because of participating a common lineage, convergent evolution and selection of superior genotypes by farmers.…”

Section: Resultssupporting

confidence: 85%

Genetic characterization of Iranian safflower (Carthamus tinctorius) using inter simple sequence repeats (ISSR) markers

Panahi

Neghab

2013

Physiol Mol Biol Plants

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Safflower (Carthamus tinctorious L.) is valued as a source of high quality vegetable oil. 20 ISSR primers were used to assess the genetic diversity of 18 accessions of safflower collected from different geographical regions of Iran. The ISSR primers combinations revealed 57.6 % polymorphism, among 338 genetic loci amplified from the accessions. The sum of effective number of alleles and observed number of alleles were 29.76 and 36.77, respectively. To understand genetic relationships among these cultivars, Jacquards' similarity coefficient and UPGMA clustering algorithm were applied to the ISSR marker data set. ISSR markers grouped accessions into two main clusters and four sub clusters. Also, the principal coordinate analysis (PCoA) supported the cluster analysis results. The results showed these genotypes have high genetic diversity, and can be used for alternative safflower breeding program.

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Section: Resultssupporting

confidence: 85%

Genetic characterization of Iranian safflower (Carthamus tinctorius) using inter simple sequence repeats (ISSR) markers

Panahi

Neghab

2013

Physiol Mol Biol Plants

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“…In our research, the most polymorphic bands was associated to (CA)n, (AC)n, and (AG)n primers. This is in agreement with the findings of a previous study on safflower having also indicated that (AG)n and (CA)n ISSR primers created the highest polymorphic bands (Golkar et al, 2011). These motifs might have a significant contribution in the safflower genome.…”

Section: Discussionsupporting

confidence: 83%

Genetic Diversity Analysis of Safflower (Carthamus tinctorius) Accessions from Different Geographic Origins using ISSR Markers

Houmanat¹,

Charafi²,

Mazouz³

et al. 2016

IJAB

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Safflower (Carthamus tinctorius) is an oilseed crop with high quality vegetable seed oil. A set of 55 accessions from different geographical origins were introduced to constitute a breeding program germplasm. These materials were characterized by inter-simple sequence repeat (ISSR) as a simple method to reveal polymorphism among them. ISSR analysis was carried out with 13 primers. After agarose gels analysis, ISSR banding patterns were converted into binary data of presence-non presence and matrices were processed. Genetic distances between all pairwise combinations of the accessions were calculated using similarity of simple coefficient matching (SCM). Dendrogram of studied accessions was constructed using the UPGMA (unweighted pair group method arithmetic average) method. A high number of reproducible ISSR bands exhibited a high percentage of polymorphism (69.64%), indicating a high genetic diversity among the material studied. The lowest similarity coefficient was 0.462, observed between accessions 109 and 135, whilst the highest one was 0.962, observed between accessions 68 and 126, with an average of 0.726. Dendrogram similarity relationships revealed some clusters according to different genetic pools. The ISSR markers were very informative and exhibited a high level of polymorphism. The findings of this study would be useful for safflower breeding program in Morocco as well as in other countries of the world.

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“…Of 268 fragments produced, 264 polymorphic bands (98.5%) were used to evaluate genetic relationships between the three varieties of L. pumila Benth (alata, pumila, lanceolata) and L. paucifolia (Table 2). The percentage of polymorphism in this study was higher than that reported by previous studies using RAPD (63.4%) (Rafii et al, 2012) and ISSR (0.73%) analysis (Golkar et al, 2011). The number of alleles varied from 5 (I3) to 25 (I2) fragments per primer and ranged from 160 to 2218 bp in size.…”

Section: Resultscontrasting

confidence: 53%

Genetic relationship among Labisia pumila (Myrsinaceae) species based on ISSR-PCR

Karimi¹,

Jaafar²,

Aziz³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The genus Labisia (Myrsinaceae) is a popular medicinal plant in Malaysia. We examined the genetic relationship among three varieties of L. pumila var. pumila, L. pumila var. alata, L. pumila var. lanceolata and Labisia paucifolia using an ISSR assay. Fifty-eight primers were tested, among which 18 gave reliable polymorphic banding patterns; these yielded 264 polymorphic markers. A similarity matrix was used to construct a dendrogram, and a principal component plot was developed to examine genetic relationships among varieties. Jaccard's similarity coefficient among species ranged from 0.09 to 0.14. At a similarity of 0.117%, species were divided into two main clusters. The mean value of the observed number of alleles, the effective number of alleles, mean Nei's gene diversity, and Shannon's information index were 1.98, 1.64, 0.38, and 0.57, respectively.

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Genetic Variation in Safflower (Carthamus tinctorious L.) for Seed Quality-Related Traits and Inter-Simple Sequence Repeat (ISSR) Markers

Cited by 70 publications

References 25 publications

Genetic characterization of Iranian safflower (Carthamus tinctorius) using inter simple sequence repeats (ISSR) markers

Genetic characterization of Iranian safflower (Carthamus tinctorius) using inter simple sequence repeats (ISSR) markers

Genetic Diversity Analysis of Safflower (Carthamus tinctorius) Accessions from Different Geographic Origins using ISSR Markers

Genetic relationship among Labisia pumila (Myrsinaceae) species based on ISSR-PCR

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