Genetic Variation in Putative Salt Taste Receptors and Salt Taste Perception in Humans

Dias, Adriano Basso; Rousseau, Dérick; Duizer, Lisa; Cockburn, Moira; Chiu, Winnie; Nielsen, Daiva E.; El-Sohemy, Ahmed

doi:10.1093/chemse/bjs090

Cited by 78 publications

(80 citation statements)

References 24 publications

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“…Genetic variations in taste receptors have been reported to be associated with modifying bitter, sweet, and salty taste. People with the T allele of TRPV1 rs8065080 are significantly more sensitive to salt solution than those with the C allele [20]. However, the present study showed that subjects with minor alleles of TRPV1 rs161364 and rs8065080 exhibited a preference for sweet tastes but not for other tastes.…”

Section: Discussioncontrasting

confidence: 73%

“…TRPV1 is not a taste receptor but a pain receptor. However, ‘spicy hot' is sensed by TRPV1, and is reported to be a similar taste sensation to that of salt [20]. Perceptions of salty and sour appear to be related to ligand-gated transmembrane channels, whereas other taste perceptions including spicy are associated with the family of G protein-coupled receptors [35].…”

Section: Discussionmentioning

confidence: 99%

“…TRPV1 agonists such as resiniferatoxin and capsaicin enhance the response to Na + , K + , and NH 4 + salts, whereas TRPV1 antagonists such as SB-366791, iodoresiniferatoxin, and capsazepine suppress the response [17,18,19]. Furthermore, the TT+TC genotype of TRPV1 rs8065080 (C>T, Val585Ile) is significantly more sensitive to salt solutions than the CC genotype (98.3 ± 3.8 vs. 74.1 ± 8.3 on general labeled magnitude scales, p = 0.008) [20]. Thus, the TRPV1 genotypes can affect the sensation of taste, especially salts, and may interact with taste preference and food intake.…”

Section: Introductionmentioning

confidence: 99%

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TRPV1 Gene Polymorphisms Are Associated with Type 2 Diabetes by Their Interaction with Fat Consumption in the Korean Genome Epidemiology Study

Park

Zhang²,

Lee³

et al. 2016

Lifestyle Genomics

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Background: Different transient receptor potential vanilloid 1 (TRPV1) variants may be differently activated by noxious stimuli. Aim: We investigated how TRPV1 variants modulated the prevalence of type 2 diabetes and specific gene-nutrient interactions. Methods: Among 8,842 adults aged 40-69 years in the Korean Genome Epidemiology Study, the associations between TRPV1 genotypes and the prevalence of type 2 diabetes as well as their gene-nutrient interactions were investigated after adjusting for the covariates of age, gender, residence area, body mass index, daily energy intake, and total activity. Results: The TRPV1 rs161364 and rs8065080 minor alleles lowered HOMA-IR and the risk of type 2 diabetes after adjusting for covariates. There were gene-nutrient interactions between TRPV1 variants rs161364 and rs8065080 and preference for oily taste, intake of oily foods, and fat intake after adjusting for covariates. Among subjects with the minor alleles of TRPV1 rs161364 and rs8065080, the group with a high preference for oily foods had a lower odds ratio for type 2 diabetes. Consistent with the preference for taste, among subjects with the minor alleles, the group with high fat intake from oily foods also exhibited a lower risk of type 2 diabetes than subjects with the major alleles. Conclusions: People with the minor alleles of the TRPV1 single nucleotide polymorphisms rs161364 and rs8065080 have a lower risk of diabetes with a high-fat diet, but people with the major alleles are at a higher risk of type 2 diabetes when consuming high-fat diets. The majority of people should be careful about a high fat intake.

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Section: Discussioncontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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TRPV1 Gene Polymorphisms Are Associated with Type 2 Diabetes by Their Interaction with Fat Consumption in the Korean Genome Epidemiology Study

Park

Zhang²,

Lee³

et al. 2016

Lifestyle Genomics

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“…Detection thresholds for sucrose were assessed using a three-alternative forced choice up down method [18]. Participants were asked to rinse their mouth with distilled water prior to the start of the test and between trials.…”

Section: Methodsmentioning

confidence: 99%

Variation in the TAS1R2 Gene, Sweet Taste Perception and Intake of Sugars

Dias

Eny²,

Cockburn³

et al. 2015

Lifestyle Genomics

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Background/Aims: To determine whether variation in the TAS1R2 gene affects sucrose taste perception and sugar intake. Methods: Participants were men (n = 238) and women (n = 458) aged 20-29 years. A subset (n = 95) with body mass index (BMI) data available completed a sensory analysis study. A food frequency questionnaire assessed dietary intake, and eight polymorphisms were genotyped (rs12033832, rs12137730, rs35874116, rs3935570, rs4920564, rs4920566, rs7513755 and rs9701796). Sucrose taste thresholds were determined by staircase procedure (solutions: 9 × 10^-6 to 0.5 mol/l). Suprathreshold sensitivity to 0.01-1.0 mol/l sucrose solutions was assessed using general Labeled Magnitude Scales. Results: A significant genotype-BMI interaction was observed for rs12033832 (G>A) for suprathreshold sensitivity (p = 0.01) and sugar intake (p = 0.003). Among participants with a BMI ≥25, G allele carriers had lower sensitivity ratings (mean incremental area under the taste sensitivity curve ± SE; GG/GA 54.4 ± 4.1 vs. AA 178.5 ± 66.6; p = 0.006), higher thresholds (GG/GA 9.3 ± 1.1 vs. AA 4.4 ± 4.3 mmol/l; p = 0.004) and consumed more sugars (GG/GA 130 ± 4 vs. AA 94 ± 13 g/day; p = 0.009). G allele carriers with a BMI <25 had lower thresholds (GG/GA 8.6 ± 0.5 vs. AA 16.7 ± 5.7 mmol/l; p = 0.02) and consumed less sugars (GG/GA 122 ± 2 vs. AA 145 ± 8 g/day; p = 0.004). Conclusion: The rs12033832 single nucleotide polymorphism in TAS1R2 is associated with sucrose taste and sugar intake, but the effect differs depending on BMI.

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“…TRPV1 expression has been observed immunohistochemically in primary afferents and also in oral epithelial cells in the rat tongue (18,21) and in human taste cell lines from lingual epithelium by RT-PCR (16,17). A recent study identified TRPV1 rs8065080 (C Ͼ T) singlenucleotide polymorphism as a likely modifier of an individual's perception of salt at suprathreshold levels (12).…”

mentioning

confidence: 99%

TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response

Ren

Rhyu

Phan

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

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Ren Z, Rhyu M, Phan TT, Mummalaneni S, Murthy KS, Grider JR, DeSimone JA, Lyall V. TRPM5-dependent amilorideand benzamil-insensitive NaCl chorda tympani taste nerve response. Am J Physiol Gastrointest Liver Physiol 305: G106 -G117, 2013. First published May 2, 2013; doi:10.1152/ajpgi.00053.2013.-Transient receptor potential (TRP) subfamily M member 5 (TRPM5) cation channel is involved in sensing sweet, bitter, umami, and fat taste stimuli, complex-tasting divalent salts, and temperature-induced changes in sweet taste. To investigate if the amiloride-and benzamil (Bz)-insensitive NaCl chorda tympani (CT) taste nerve response is also regulated in part by TRPM5, CT responses to 100 mM NaCl ϩ 5 M Bz (NaCl ϩ Bz) were monitored in Sprague-Dawley rats, wild-type (WT) mice, and TRP vanilloid subfamily member 1 (TRPV1) and TRPM5 knockout (KO) mice in the presence of resiniferatoxin (RTX), a TRPV1 agonist. In rats, NaCl ϩ Bz ϩ RTX CT responses were also monitored in the presence of triphenylphosphine oxide, a specific TRPM5 blocker, and capsazepine and N-(3-methoxyphenyl)-4-chlorocinnamid (SB-366791), specific TRPV1 blockers. In rats and WT mice, RTX produced biphasic effects on the NaCl ϩ Bz CT response, enhancing the response at 0.5-1 M and inhibiting it at Ͼ1 M. The NaCl ϩ Bz ϩ SB-366791 CT response in rats and WT mice and the NaCl ϩ Bz CT response in TRPV1 KO mice were inhibited to baseline level and were RTX-insensitive. In rats, blocking TRPV1 by capsazepine or TRPM5 by triphenylphosphine oxide inhibited the tonic NaCl ϩ Bz CT response and shifted the relationship between RTX concentration and the magnitude of the tonic CT response to higher RTX concentrations. TRPM5 KO mice elicited no constitutive NaCl ϩ Bz tonic CT response. The relationship between RTX concentration and the magnitude of the tonic NaCl ϩ Bz CT response was significantly attenuated and shifted to higher RTX concentrations. The results suggest that pharmacological or genetic alteration of TRPM5 activity modulates the Bz-insensitive NaCl CT response and its modulation by TRPV1 agonists. triphenylphosphine oxide; resiniferatoxin; capsazepine; SB-366791; TRPV1 APPETITIVE AND AVERSIVE NEURAL and behavioral responses to NaCl are most likely transduced by a variety of mechanisms (35). Several studies suggest that in the anterior tongue Na ϩ from a Na ϩ salt taste stimulus enters a subset of taste bud cells by at least two types of cation channels located in taste cell apical membranes. One channel type is the Na ϩ -specific epithelial Na ϩ channel (ENaC), in which Na ϩ transport is inhibited by amiloride and benzamil (Bz) (1, 3, 8). The second involves Na ϩ transport through a putative transient receptor potential (TRP) vanilloid subfamily member 1 (TRPV1t)-nonspecific cation channel (26,27). The TRPV1t channel is insensitive to amiloride and Bz, permeable to Na ϩ , K ϩ , NH 4 ϩ , and Ca 2ϩ , and contributes to cell depolarization (31). These conclusions are supported by the observations that the phasic (transient) and tonic (steady-state) components of ...

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Genetic Variation in Putative Salt Taste Receptors and Salt Taste Perception in Humans

Cited by 78 publications

References 24 publications

<b><i>TRPV1</i></b> Gene Polymorphisms Are Associated with Type 2 Diabetes by Their Interaction with Fat Consumption in the Korean Genome Epidemiology Study

<b><i>TRPV1</i></b> Gene Polymorphisms Are Associated with Type 2 Diabetes by Their Interaction with Fat Consumption in the Korean Genome Epidemiology Study

Variation in the <b><i>TAS1R2</i></b> Gene, Sweet Taste Perception and Intake of Sugars

TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response

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