Genetic variability among Anopheles species belonging to the Nyssorhynchus and Anopheles subgenera in the Amazon region

Moroni, Raquel Borges; Maia, Juracy de Freitas; Tadei, Wanderli Pedro; Santos, Joselita Maria Mendes dos

doi:10.1590/s0037-86822010000400015

Cited by 4 publications

(3 citation statements)

References 31 publications

(42 reference statements)

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“…Genetic variations among An. darlingi populations also have been demonstrated by analysis of polytene chromosomes, isozymes, and nuclear and mitochondrial DNA markers (Tadei et al 1982, Malafronte et al 1999, Manguin et al 1999, Mirabello et al 2008, Pedro and Sallum 2009, Moroni et al 2010, Moreno et al 2010. Despite the observed differences among examined populations, An.…”

Section: Discussionmentioning

confidence: 92%

Multiple blood meals inAnopheles darlingi(Diptera: Culicidae)

Oliveira¹,

Tadei²,

Abdalla³

et al. 2012

Journal of Vector Ecology

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Anopheles darlingi is an important vector of human malaria in the Amazon. Adult females of this mosquito species require a blood meal to develop eggs, preferring humans to other blood sources. Although gonotrophic concordance has been described as the norm for An. darlingi, here we report An. darlingi female mosquitoes taking two or more blood meals within their first gonotrophic cycle. Only half of field-captured adult females fed one blood meal developed follicles to Christophers' stage V. This outcome is dependent on larval nutrition, as 88% of laboratory-raised well-nourished females completed the first gonotrophic cycle with only one blood meal, while less nourished females needed additional blood meals. Half of the fieldcaptured blood-seeking An. darlingi females had follicles in intermediate (IIIa and IIIb) and final (V) stages of the gonotrophic cycle, supporting the conclusion that An. darlingi blood feed more than once during a gonotrophic cycle. Additionally, we observed females attempting to blood feed a second time during the same day. Additional studies of An. darlingi biting behavior are necessary to accurately estimate Plasmodium sp. entomologic inoculation rates throughout the An. darlingi vast geographical distribution. Journal of Vector Ecology 37 (2): 351-358. 2012.

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Section: Discussionmentioning

confidence: 92%

Multiple blood meals inAnopheles darlingi(Diptera: Culicidae)

Oliveira¹,

Tadei²,

Abdalla³

et al. 2012

Journal of Vector Ecology

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“…In Brazil, there are about 60 species of Anopheles [ 8 ], of which An. darlingi is considered to be the main vector of human malaria [ 9 ], mostly in the Amazon region [ 10 ]. However, other species belonging to the subgenus Nyssorhynchus are also involved in the transmission of malaria in the Amazon, including An.…”

Section: Introductionmentioning

confidence: 99%

Outbreak of autochthonous cases of malaria in coastal regions of Northeast Brazil: the diversity and spatial distribution of species of Anopheles

et al. 2020

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Background Brazil has the fourth highest prevalence of malaria of all countries in the Americas, with an estimated 42 million people at risk of contracting this disease. Although most cases occur in the Amazon region, cases of an autochthonous nature have also been registered in the extra-Amazonian region where Anopheles aquasalis and An. albitarsis are the mosquito species of greatest epidemiological interest. In 2019, the municipality of Conde (state of Paraíba) experienced an epidemic of autochthonous cases of malaria. Here we present preliminary results of an entomological and case epidemiology investigation, in an attempt to correlate the diversity and spatial distribution of species of Anopheles with the autochthonous cases of this outbreak of malaria. Methods Case data were collected using case report forms made available by the Conde Municipal Health Department. The entomological survey was carried out from July to November 2019. The various methods of capture included the use of battery-powered aspirators, mouth aspirators, Shannon traps, BG-Sentinel traps (with and without dry ice) and CDC light traps. Captured mosquitoes were separated, packaged and sent to the laboratory for sexing and molecular identification of the various species of anophelines. The data were tabulated and analyzed using Microsoft Excel. Spatial analysis of the data was performed using ArcGis 10 software. Results In 2019, 20 autochthonous cases and one imported case of malaria caused by Plasmodium vivax were diagnosed, with three cases of relapses. A total of 3713 mosquitoes were collected, of which 3390 were culicines and 323 were anophelines. Nine species of genus Anopheles were identified, with the most abundant being An. aquasalis (38.9%), followed by An. minor (18.2%) and An. albitarsis (9.0%). Spatial analysis of the data showed that the area could be considered to be at risk of malaria cases and that there was a high prevalence of Anopheles. Conclusions The results presented indicate that this extra-Amazonian region has an environment conducive to maintenance of the malaria transmission cycle owing to the wide diversity of Anopheles species. This environment in combination with the high influx of people from endemic areas to the study area provides a perfect setting for the occurrence and maintenance of malaria.

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“…The Nyssorhynchus subgenus is currently comprised of 39 formally recognized species, which includes the main malaria vector in Brazil An. darlingi (Harbach, 2014;Moroni et al, 2010;Martins-Campos et al). The Nyssorhynchus subgenus is further divided into 3 sections: the Albimanus Section (Faran, 1980), Argyritarsis Section (Linthicum, 1988) and the Myzorhynchella Section (Galvão, 1941).…”

Section: Family Culicidaementioning

confidence: 99%

Análise multigênica e distribuição especial de espécies do Subgrupo Strodei de Anopheles (Nyssorhynchus) (Diptera: Culicidae)

Greni¹

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Introduction Anopheles strodei sensu lato is an understudied subgroup of potential epidemiological importance, having been found naturally infected in Brazil with Plasmodium falciparum, Plasmodium vivax and Plasmodium malariae. An. strodei s.l. is currently composed on 8 species: An. albertoi Unti, An. CP Form, An. rondoni (Neiva & Pinto), An. strodei Root, An. arthuri Unti and three other unnamed species that have been proposed by Bourke et al. (2013): An. arthuri B, An. arthuri C and An. arthuri D. Objectives As delineating species accurately is an essential goal of public health entomology, the objectives of this study were to: 1) Determine the phylogenetic relationships within the Strodei Subgroup and reaffirm or reject the hypothesis of the 3 new species (An. arthuri B, An. arthuri C and An. arthuri D) 2) Address the potential spatial distribution of species of the An. strodei subgroup to provide support for the candidate species in the Strodei Subgroup Methods Bayesian inference, which included DNA sequences of one mitochondrial and three nuclear protein coding genes: CO1, white, CAD and CAT, was used to determine the phylogenetic relationship within the group. To propose a species distribution, collection localities, along with climatic and geographic data were input into MAXENT. Results When analyzing the four molecular markers employed, support was found for allopatry in the Strodei Subgroup. The paraphyletic clade of An. arthuri was supported. Conclusion Potential species distributions of the Strodei Subgroup were addressed for the first time. Fifty-five unique CAT sequences and 46 unique CAD sequences were newly characterized.

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Genetic variability among Anopheles species belonging to the Nyssorhynchus and Anopheles subgenera in the Amazon region

Cited by 4 publications

References 31 publications

Multiple blood meals inAnopheles darlingi(Diptera: Culicidae)

Multiple blood meals inAnopheles darlingi(Diptera: Culicidae)

Outbreak of autochthonous cases of malaria in coastal regions of Northeast Brazil: the diversity and spatial distribution of species of Anopheles

Análise multigênica e distribuição especial de espécies do Subgrupo Strodei de Anopheles (Nyssorhynchus) (Diptera: Culicidae)

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