Genetic Typing of Feline Rabies Virus Isolated in Greater Bangkok, Thailand

Kasempimolporn, Songsri; Saengseesom, Wachiraporn; Tirawatnapong, Thaweesak; Puempumpanich, S; Sitprija, Visith

doi:10.1111/j.1348-0421.2004.tb03511.x

Cited by 6 publications

(2 citation statements)

References 13 publications

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“…Analysis of Glycoprotein gene is important to describe antigenic diversity among rabies virus species (Kasempimolporn et al 2004;Kuzmin et al 2004). Glycoprotein is directly related to virus pathogenicity and has the main role in immunogenicity against virus (Cox, Dietzschold, & Schneider, 1977;Morimoto, et al 2001).…”

Section: Research Questionsmentioning

confidence: 99%

Phylogeny Study Of Some Vaccinal Strains Of Rabies Virus And Comparing Their Antigenic Glycoprotein Specificity With The Wild Type

Ajorloo¹,

Moradi²,

Zarif³

et al. 2017

European Proceedings of Social and Behavioural Sciences

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Rabies is a viral disease causing acute encephalitis in humans or animals. Rabies virus belongs to the genus Lyssavirus, family Rabdoviridea. The virus has a single, negative-stranded RNA genome encoding five structural proteins, including nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and RNA dependent RNA polymerase gene (L). All of the provinces of Iran are infected with rabies. Although there is no cure for rabies, it is totally preventable by proper vaccination. Effective vaccine can be used for pre-and post-exposure prophylaxis. Glycoprotein has a main role in pathogenicity and immunogenicity thus variation in genetic sequence of this gene leads to variation in antigenic and pathogenic properties of rabies virus. Therefore, in this study, we analyzed the antigenic sites of the glycoprotein from rabies virus strains used in vaccine manufacture and compared their epitope sequences to wild type strain. We have used RT-PCR technique to determine the genetic sequence of these strains. Phylogenetic analysis showed that the wild type street virus isolate found in Iran were related to genotype 1 (classical rabies virus) and shared a high homology with the vaccine strains. Furthermore comparison of amino acid sequences of major and minor antigenic sites between the wild type and several vaccinal strains showed that the virus had a higher homology with the vaccinal strain PV that is used to manufacture vaccines in the country.

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Section: Research Questionsmentioning

confidence: 99%

Phylogeny Study Of Some Vaccinal Strains Of Rabies Virus And Comparing Their Antigenic Glycoprotein Specificity With The Wild Type

Ajorloo¹,

Moradi²,

Zarif³

et al. 2017

European Proceedings of Social and Behavioural Sciences

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“…Cases of rabies in domestic animals other than dogs have been traceable to, or believed to have been at the origin of exposure to, rabid dogs. 11 In a sampling survey conducted by the Bureau of National Statistics and the Bangkok Metropolitan Administration (BMA) in 1999, it was estimated that 633,814 dogs inhabit Bangkok. Additionally, an estimation of the total pet dog population was reported to be 823,504 in 2006.…”

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confidence: 99%

Evaluation of a rapid immunochromatographic test strip for detection of Rabies virus in dog saliva samples

et al. 2011

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Abstract. An immunochromatographic test strip for Rabies virus was evaluated with dog saliva samples. The test was initially validated against 237 dogs of known infection status, and then evaluated in the field with 1,290 live dogs. By validation of paired saliva-brain specimens obtained from dogs at necropsy, the saliva strip test was 94.4% specific and 93.0% sensitive when compared to the gold standard fluorescent antibody test (FAT) on brain smears. The sensitivity and specificity of a nested polymerase chain reaction (nPCR) assay using saliva were 100% compared to the FAT results. The performance of strip test with field saliva samples from street dogs had a specificity of 98.7% in comparison to nPCR as the reference method. As the strip test kit can potentially be used outside the laboratory and be applicable as an on-site testing assay, it represents a powerful screening tool for epidemiological surveys and disease control. The test could be useful for the surveillance of rabies in dogs and, in particular, be used to monitor the success of rabies control programs.

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Molecular Epidemiology

Nadin-Davis

2013

Rabies

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Genetic Typing of Feline Rabies Virus Isolated in Greater Bangkok, Thailand

Cited by 6 publications

References 13 publications

Phylogeny Study Of Some Vaccinal Strains Of Rabies Virus And Comparing Their Antigenic Glycoprotein Specificity With The Wild Type

Phylogeny Study Of Some Vaccinal Strains Of Rabies Virus And Comparing Their Antigenic Glycoprotein Specificity With The Wild Type

Evaluation of a rapid immunochromatographic test strip for detection of Rabies virus in dog saliva samples

Molecular Epidemiology

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