Genetic Transformation of Carnation (Dianthus caryophylus L.)

Nontaswatsri, Chalermsri; Fukai, Seiichi

doi:10.1007/978-1-60327-114-1_9

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…The major hurdle most of the ornamental plants encounter is sexual hybridization because of their higher heterozygosity, sterility, higher chromosome number and longer life spans. Important ornamentals such as carnations are self-fertile and unable to generate seeds, the huge genome sizes in chrysanthemums and lilies make genome mining harder, and the life cycle of anthuriums and some orchids is about 3 years, which means a longer time period is required to develop a cultivar [46][47][48]. Cross-breeding has been beneficial in developing various novel cultivars with morphological variations.…”

Section: Importance Of Improving Ornamental Attributesmentioning

confidence: 99%

Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update

Mekapogu,

Song,

Lim

et al. 2023

Plants

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The ornamental horticulture industry is a highly dynamic and rapidly changing market. Constant development of novel cultivars with elite traits is essential to sustain competitiveness. Conventional breeding has been used to develop cultivars, which is often laborious. Biotechnological strategies such as genetic engineering have been crucial in manipulating and improving various beneficial traits that are technically not possible through cross-breeding. One such trait is the highly desired blue-colored flower in roses and chrysanthemums, which can be achieved through transgenic technology. Advances in genome sequencing platforms have enhanced the opportunities to access the whole genome sequence in various ornamentals, facilitating the dissection of the molecular genetics and regulatory controls of different traits. The recent advent of genome editing tools, including CRISPR/Cas9, has revolutionized plant breeding. CRISPR/Cas9-based gene editing offers efficient and highly precise trait modification, contributing to various beneficial advancements. Although genome editing in ornamentals is currently in its infancy, the recent increase in the availability of ornamental genome sequences provides a platform to extend the frontiers of future genome editing in ornamentals. Hence, this review depicts the implication of various commercially valuable ornamental attributes, and details the research attempts and achievements in enhancing floral attributes using genetic engineering and genome editing in ornamental plants.

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Section: Importance Of Improving Ornamental Attributesmentioning

confidence: 99%

Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update

Mekapogu,

Song,

Lim

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Therefore, developing new cultivar may require long time span of about 8–10 years (Azadi et al, 2016 ). Similarly, most of the carnation lines are self-fertile and unable to produce seeds (Nontaswatsri and Fukai, 2010 ). Huge genome size in ornamental plants e.g., lilly is a hurdle in mining genomic information (Du et al, 2015 ).…”

Section: Why Floral Traits Modifications Are Of Special Concern?mentioning

confidence: 99%

Biotechnological Advancements for Improving Floral Attributes in Ornamental Plants

et al. 2017

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Developing new ornamental cultivars with improved floral attributes is a major goal in floriculture. Biotechnological approach together with classical breeding methods has been used to modify floral color, appearance as well as for increasing disease resistance. Transgenic strategies possess immense potential to produce novel flower phenotypes that are not found in nature. Adoption of Genetic engineering has supported the idea of floral trait modification. Ornamental plant attributes like floral color, fragrance, disease resistance, and vase life can be improved by means of genetic manipulation. Therefore, we witness transgenic plant varieties of high aesthetic and commercial value. This review focuses on biotechnological advancements in manipulating key floral traits that contribute in development of diverse ornamental plant lines. Data clearly reveals that regulation of biosynthetic pathways related to characteristics like pigment production, flower morphology and fragrance is both possible and predictable. In spite of their great significance, small number of genetically engineered varieties of ornamental plants has been field tested. Today, novel flower colors production is regarded as chief commercial benefit obtained from transgenic plants. But certain other floral traits are much more important and have high commercial potential. Other than achievements such as novel architecture, modified flower color, etc., very few reports are available regarding successful transformation of other valuable horticultural characteristics. Our review also summarized biotechnological efforts related to enhancement of fragrance and induction of early flowering along with changes in floral anatomy and morphology.

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The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis

Zhou

Xiao-mi

2022

BMC Plant Biol

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Background Deciphering the mechanisms of meiosis has important implications for potential applications in plant breeding programmes and species evolution. However, the process of meiosis is poorly understood in carnation, which is famous for its cut flowers. Results We report that Dianthus caryophyllus parallel spindle 1 (DcPS1) regulates omission of second division like a (OSDLa) during pollen development and 2n gamete production in carnation meiosis. In DcPS1 and OSDLa RNAi lines, an absence of the second meiotic division and the abnormal orientation of spindles at meiosis II might be the main reason for dyad/triad formation, resulting in unreduced gametes. We also found that carnation OSDLa interacted with DcPS1 and DcRAD51D. In the DcPS1 RNAi lines, a decrease in OSDLa and DcRAD51D expression was observed. In the OSDLa RNAi lines, a decrease in DcPS1 and DcRAD51D expression was also observed. We propose that DcPS1 regulates OSDLa expression, allowing entry into meiosis II and the proper orientation of the metaphase II spindle in meiosis II. We also propose that OSDLa regulates DcRAD51D expression, allowing for homologous recombination. Conclusions These results suggest a critical role for DcPS1 and OSDLa in diplogamete production during meiosis and open a new pathway for meiosis-related studies.

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Genetic Transformation of Carnation (Dianthus caryophylus L.)

Cited by 3 publications

References 15 publications

Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update

Genetic Engineering and Genome Editing Advances to Enhance Floral Attributes in Ornamental Plants: An Update

Biotechnological Advancements for Improving Floral Attributes in Ornamental Plants

The DcPS1 cooperates with OSDLa during pollen development and 2n gamete production in carnation meiosis

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