Genetic Traceability of Cinta Senese Pig (<i>Sus scrofa domesticus</i> L.): A Study of the Meat and Processed Products by Microsatellite Markers

Abstract

How to cite this paper: Scali, M., Vignani, R., Quintetti, M., Bigliazzi, J., Paolucci, E. and Cresti, M. (2015) AbstractTraceability based on DNA analysis is attracting increasing interest due to the crisis of confidence that consumers show towards the products of animal origin. The present work discusses a genetic traceability system of meat and processed products from an historical Tuscan native pig breed, the Cinta Senese. The study is based on a panel of 8 ISAG (International Society for Animal Genetics) DNA microsatellite markers usage done both on pigs and derived products. The SSRs panel allowed us to obtain a unique fingerprint of the individuals to be used as a tracer "downstream" in the processed products. The molecular method used proved that the hams, analyzed just before commercialization, were obtained from Cinta Senese pigs and that the analyzed meat products derived from the Cinta Senese were produced at least with 95% of Cinta Senese meat. In perspective, the molecular testing could be introduced as a voluntarily adopted method for proving intrinsic quality of many regional food products.

Islands as Time Capsules for Genetic Diversity Conservation: The Case of the Giglio Island Mouflon

“…To evaluate the population diversity and structure, samples were genotyped at 14 Simple Sequence Repeats (SSR) loci retrieved from literature [14]. The amplification products were obtained according to Scali and colleagues [19] and subjected to fine sizing by capillary electrophoresis using an ABI 3130 DNA analyser (Applied Biosystems). The single sample from Elba Island was not subjected to population-aimed analyses.…”