Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis

Sant’Anna, Juliane Rocha de; Miyamoto, Cláudia Tiemi; Rosada, Lúcia J; Franco, Claudinéia C. S.; Kaneshima, Edílson Nobuyoshi; Castro-Prado, Marialba Avezum Alves de

doi:10.4067/s0716-97602010000100007

Cited by 7 publications

(10 citation statements)

References 18 publications

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“…Genetic variability among nine isolates of C. truncatum in soybean was verified through RAPD profiles, where formation of the three groups was observed and correlated with phenotypic variability related to nitrate utilization by the isolates (Sant'anna et al . ). Other studies showed relation between the genetic variation in C. truncatum and the host.…”

Section: Discussionmentioning

confidence: 97%

Phylogeny and variability ofColletotrichum truncatumassociated with soybean anthracnose in Brazil

et al. 2017

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Section: Discussionmentioning

confidence: 97%

Phylogeny and variability ofColletotrichum truncatumassociated with soybean anthracnose in Brazil

et al. 2017

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“…Soybean anthracnose is an economically important disease that is distributed in soybean-growing areas worldwide, causing considerable losses in soybean production, especially in warm, humid weather (Sant'Anna et al, 2010). The most common pathogen associated with soybean anthracnose is Colletotrichum truncatum (Schwein.)…”

Section: Introductionmentioning

confidence: 99%

CtPMK1, a mitogen‐activated‐protein kinase gene, is required for conidiation, appressorium formation, and pathogenicity of Colletotrichum truncatum on soybean

Xiong

Zhao

et al. 2015

Annals of Applied Biology

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Colletotrichum truncatum, the causal agent of soybean anthracnose, invades host plants by forming a specialised infection structure called an appressorium. Mitogen-activated protein kinase (MAPK) genes have been shown to play vital roles in several phytopathogenic fungi in regulating various infection processes, including spore germination, melanised appressorium formation, appressorial penetration and subsequent invasive growth in host plants. In this study, we identified and characterised the first Fus3/Kss1-related MAPK gene, CtPMK1, in Colletotrichum truncatum, which is related to PMK1 in Magnaporthe oryzae. Disruption of CtPMK1 in C. truncatum resulted in a mutant with slightly reduced mycelial growth (-30%) and melanisation that is deficient in sporulation (-99%), as observed in the CMK1 mutant of Colletotrichum lagenarium (a synonym of Colletotrichum orbiculare, which is now the accepted name for this taxon). In contrast to CMK1 of C. lagenarium, conidia from the Ctpmk1 mutant germinated normally on glass slides and onion epidermal surfaces. Our findings suggest that there are differences in the types of in vitro functions controlled by PMK1, even between closely related species. Furthermore, the Ctpmk1 mutant failed to form appressoria or hyphopodia, subsequently resulting in the complete loss of pathogenicity on host plants. Overall, the results indicate that the Fus3/Kss1-related MAPK gene has a conserved role in infection structure formation and pathogenicity in phytopathogenic fungi.

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“…Similar results were previously observed in the phytopathogens Colletotrichum truncatum and C. acutatum . Whereas in C. truncatum the isolates B and E, obtained respectively from the Brazilian states of Paraná and Minas Gerais, were allocated in distinct VCGs (Sant'Anna et al ., ), in C. acutatum, isolates from different locations and hosts (strawberry and Tahiti lime) were assigned to the same VCG (Franco et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Heterokaryosis and diploid formation among Brazilian isolates of Macrophomina phaseolina

et al. 2018

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Heterokaryosis is the association of genetically distinct nuclei in a common hyphal cytoplasm, and is a process involved in the generation of fungal variation. The fusion of the distinct nuclei within a heterokaryotic hypha produces a heterozygous diploid nucleus and is one of the stages of the parasexual cycle. A heterokaryon's viability depends on the isolates’ genetic constitution with regard to loci called het (heterokaryon incompatibility) or vic (vegetative incompatibility). The current study evaluates for the first time the diversity of vegetative compatibility reactions in isolates of Macrophomina phaseolina from different hosts. Complementary nit (nitrate non‐utilizing) mutants of each isolate were obtained and paired in all possible combinations. Isolates were classified in vegetative compatibility groups (VCG) according to their ability to form viable heterokaryons. Ten VCGs were identified, two of them containing two isolates, and the remainder containing a single isolate. When grown on basal medium, heterokaryons produced both (i) auxotrophic segregants exhibiting the same phenotype as the paired mutants; and (ii) a fast‐growing sector, characterized as a heterozygous diploid sector, named D653, which showed a nit+ phenotype with a growth rate similar to the original wild isolate. When grown in the presence of benomyl, a haploidizing agent, D653 produced auxotrophic haploid segregants exhibiting the nit phenotypes of the crossed mutants. The results demonstrate for the first time the ability of M. phaseolina isolates to form viable heterokaryons and heterozygous diploid nuclei, suggesting that the parasexual cycle may be an alternative source of genetic variability in this species.

show abstract

Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis

Cited by 7 publications

References 18 publications

Phylogeny and variability ofColletotrichum truncatumassociated with soybean anthracnose in Brazil

Phylogeny and variability ofColletotrichum truncatumassociated with soybean anthracnose in Brazil

CtPMK1, a mitogen‐activated‐protein kinase gene, is required for conidiation, appressorium formation, and pathogenicity of Colletotrichum truncatum on soybean

Heterokaryosis and diploid formation among Brazilian isolates of Macrophomina phaseolina

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