Genetic rearrangements in the rfb regions of Vibrio cholerae O1 and O139.

Stroeher, Uwe H.; Jedani, Kathy E; Dredge, B Kate; Morona, Renato; Brown, Melissa H.; Karageorgos, Litsa; Albert, M. John; Manning, Paul A.

doi:10.1073/pnas.92.22.10374

Cited by 85 publications

(88 citation statements)

References 28 publications

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“…A deletion in O1 El Tor C31 compared to other O1 V. cholerae in a region now identified as the MGC array has been reported in earlier studies (Barker & Clark, 1994;van Dongen & DeGraaf, 1986). Earlier studies have concluded that, unlike O139 Bengal strains, Arg3 is not derived from a V. cholerae O1 parent and has evolved independently (Stroeher et al, 1995(Stroeher et al, , 1997. This is supported by the recA phylogenetic analysis (Stine et al, 2000;Fig.…”

Section: Validation Of the Gene Cassette Size Class Profiling Techniquesupporting

confidence: 69%

Use of chromosomal integron arrays as a phylogenetic typing system for Vibrio cholerae pandemic strains

et al. 2007

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Approximately 200 serogroups of Vibrio cholerae exist, with only two, O1 and O139, responsible for epidemic and pandemic cholera. Strains from these serogroups have evolved from a common progenitor, with lateral gene transfer largely driving their emergence. These strains are so closely related that separation using single-or multi-locus phylogeny has proven difficult. V. cholerae strains contain a genetic system called the integron that is located in the chromosome and that can integrate and excise DNA elements called mobile gene cassettes (MGCs) by site-specific recombination. Large arrays of MGCs are found in V. cholerae strains. For instance, the O1 El Tor strain N16961 contains 179 MGCs. Since integron arrays are dynamic through recombination and excision of MGCs, it was hypothesized that the MGC composition in a given V. cholerae pandemic strain would be useful as a phylogenetic typing system. To address this, a PCR-based method was used to rapidly characterize the MGC composition of V. cholerae arrays. The results showed that the MGC composition of pandemic V. cholerae cassette arrays is relatively conserved, providing further evidence that these strains have evolved from a common progenitor. Comparison of MGC composition between the V. cholerae pandemic strains was also able to resolve the evolution of O139 from a subgroup of O1 El Tor. This level of differentiation of closely related V. cholerae isolates was more sensitive than conventional single-gene phylogeny or multi-locus sequence analysis. Using this method, novel MGCs from an O1 classical strain and an Argentinian O139 isolate were also identified, and a major deletion in the MGC array in all pandemic O139 strains and a subset of O1 El Tor strains was identified. Analysis of sequenced V. cholerae integron arrays showed that their evolution can proceed by rearrangements and deletions/insertions of large portions of MGCs in addition to the insertion or excision of single MGCs.

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Section: Validation Of the Gene Cassette Size Class Profiling Techniquesupporting

confidence: 69%

Use of chromosomal integron arrays as a phylogenetic typing system for Vibrio cholerae pandemic strains

et al. 2007

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“…This finding is based first on the emergence of serogroup O139 (Bengal) in Bangladesh (CT positive) and Argentina (CT negative), each of which clearly evolved independently 33 . Second, the isolates from the sixth and seventh pandemic, and U.S. Gulf Coast belong to three different clones, each independently, evolved from environmental non-O1 V. cholerae isolates 16 .…”

Section: Discussionmentioning

confidence: 99%

Distribution of virulence markers in clinical and environmental Vibrio cholerae non-O1/non-O139 strains isolated in Brazil from 1991 to 2000

Theophilo

Rodrigues

Leal

et al. 2006

Rev. Inst. Med. trop. S. Paulo

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SUMMARYOne hundred seventy nine Vibrio cholerae non-O1/non-O139 strains from clinical and different environmental sources isolated in Brazil from 1991 to 2000 were serogrouped and screened for the presence of four different virulence factors. The Random Amplification of Polymorphic DNA (RAPD) technique was used to evaluate the genetic relatedness among strains. Fifty-four different serogroups were identified and V. cholerae O26 was the most common (7.8%). PCR analysis for three genes (ctxA, zot, ace) located of the CTX genetic element and one gene (tcpA) located on the VPI pathogenicity island showed that 27 strains harbored one or more of these genes. Eight (4.5%) strains possessed the complete set of CTX element genes and all but one of these belonged to the O26 serogroup suggesting that V. cholerae O26 has the potential to be an epidemic strain. The RAPD profiles revealed a wide variability among strains and no genetic correlation was observed.

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“…et al., 1991, 1995aStroeher et al, 1992Stroeher et al, , 1995a) and a defective insertion-sequence-like element, IS1358d1, has been identified (Stroeher et al, 1995a). Interestingly, this element was also found linked to the rfb operon of the recently emerged V. cholerae serogroup O139 (Stroeher et al, 1995a).…”

Section: Introductionmentioning

confidence: 98%

“…Interestingly, this element was also found linked to the rfb operon of the recently emerged V. cholerae serogroup O139 (Stroeher et al, 1995a). IS1358 contains a putative transposase (tnpA) and has all the features of a transposable element (Stroeher et al, 1995a).…”

Section: Introductionmentioning

confidence: 99%

Distribution of IS1358 and linkage to rfb-related genes in Vibrio anguillarum The GenBank accession numbers for the IS1358 sequences are U93587–U93597.

2000

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The insertion sequence IS1358 is linked to the rfb regions of both Vibrio cholerae O1 and O139, and its location was suggestive of a role in generating new combinations of rfb genes. This provoked an examination of the distribution and localization of IS1358 in Vibrio anguillarum. IS1358 was widely distributed in a number of V. anguillarum serogroups. In particular, when cosmid clones of V. anguillarum O1 were screened with IS1358 and subsequently subcloned and sequenced, it was found that rfb-like genes were linked to this region. Furthermore, when the previously identified genes virA and virB from V. anguillarum O1, now known to be involved in LPS biosynthesis, were used as probes, it was discovered that they too are present on the same large EcoRI fragment as IS1358. This clearly indicated that IS1358 was linked to the rfb region of V. anguillarum O1. Further analysis of the location of IS1358 in other serotypes indicated that V. anguillarum O2 also has IS1358 associated with rfb-like genes. In V. anguillarum O2 there is more than one copy of IS1358, suggesting that this element is a site for recombination, gene duplication or that it may be capable of transposition. Following this latter premise, IS1358 elements from a variety of V. anguillarum strains have been cloned and sequenced. Only those strains with multiple copies of IS1358 produce a full-length putative transposase, as shown by protein overexpression, further strengthening the argument that the element is transposing within these strains.

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Genetic rearrangements in the rfb regions of Vibrio cholerae O1 and O139.

Cited by 85 publications

References 28 publications

Use of chromosomal integron arrays as a phylogenetic typing system for Vibrio cholerae pandemic strains

Use of chromosomal integron arrays as a phylogenetic typing system for Vibrio cholerae pandemic strains

Distribution of virulence markers in clinical and environmental Vibrio cholerae non-O1/non-O139 strains isolated in Brazil from 1991 to 2000

Distribution of IS1358 and linkage to rfb-related genes in Vibrio anguillarum The GenBank accession numbers for the IS1358 sequences are U93587–U93597.

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