Genetic markers in blue crabs (Callinectes sapidus) I: Isolation and characterization of microsatellite markers

Steven, Colin; Hill, Jessica M.; Masters, Brian S.; Place, Allen R.

doi:10.1016/j.jembe.2004.04.020

Cited by 14 publications

(12 citation statements)

References 23 publications

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“…Heterozygosity and allelic diversity was also lower than those found by Steven et al . [ 12 ], as well as those reported for Callinectes danae at the same region studied in the present work [ 45 ]. Deviations from HWE observed in several groups indicate significant inbreeding.…”

Section: Discussionsupporting

confidence: 90%

“…However, this diversity was lower than the one reported by Steven et al . [ 12 ] for blue crabs from two locations of Chesapeake Bay, in the Western North Atlantic: 263 alleles in 134 individuals over the same seven loci. Heterozygosity and allelic diversity was also lower than those found by Steven et al .…”

Section: Discussionmentioning

confidence: 99%

“…Due to their high polymorphism, these markers are very useful in differentiating populations and inferring dispersal patterns [ 8 – 10 ]. For instance, microsatellites have been employed to better understand populations of animals with highly dispersive larvae such as the prawn Penaeus monodon [ 10 ] and the crab Carcinus maenas [ 11 ], as well the blue crab Callinectes sapidus [ 12 ]. This information can be applied to management strategies, since genetically structured populations should be considered separate management units for maintenance of genetic diversity [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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High Connectivity among Blue Crab (Callinectes sapidus) Populations in the Western South Atlantic

et al. 2016

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Population connectivity in the blue crab Callinectes sapidus was evaluated along 740 km of the Western South Atlantic coast. Blue crabs are the most exploited portunid in Brazil. Despite their economic importance, few studies report their ecology or population structure. Here we sampled four estuarine areas in southern Brazil during winter 2013 and summer 2014 in order to evaluate diversity, gene flow and structure of these populations. Nine microsatellite markers were evaluated for 213 adult crabs, with identification of seven polymorphic loci and 183 alleles. Pairwise FST values indicated low population structure ranging from -0.00023 to 0.01755. A Mantel test revealed that the geographic distance does not influence genetic (r = -0.48), and structure/migration rates confirmed this, showing that even the populations located at the opposite extremities of our covered region presented low FST and exchanged migrants. These findings show that there is a significant amount of gene flow between blue crab populations in South Brazil, likely influenced by local current dynamics that allow the transport of a high number of larvae between estuaries. Considering the elevated gene flow, the populations can be considered a single genetic stock. However, further information on population size and dynamics, as well as fishery demands and impacts at different regions, are necessary for harvest management purposes.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High Connectivity among Blue Crab (Callinectes sapidus) Populations in the Western South Atlantic

et al. 2016

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“…For all polymorphic genomic SSR loci, 489 different alleles were amplified and detected, with an average of 7.0 alleles/locus. The locus CFZB113 had the most alleles (16), while the least number of alleles (3) was detected at six loci ( Table 2). Lower variability was detected for the EST SSRs.…”

Section: Characterization Of Microsatellite Markersmentioning

confidence: 99%

Characterization of 95 novel microsatellite markers for Zhikong scallop Chlamys farreri using FIASCO-colony hybridization and EST database mining

Zhan

Bao

et al. 2008

Fisheries Sci

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In order to construct a simple sequence repeat (SSR)-based genetic linkage map and to promote molecular marker-assisted selection (MAS) in scallop breeding, the methods of Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO)-colony hybridization and expressed sequence tag (EST) database mining were modified and used to develop 95 novel microsatellite markers for Zhikong scallop. The SSR-enriched library constructed by the FIASCO method consisted of 830 clones, and 295 (35.5%) positive clones were identified after colony hybridization. One hundred and fifty clones were randomly sequenced and the results showed all clones contained at least one microsatellite. Of 91 primer pairs designed, 72 were amplified scorable polymerase chain reaction (PCR) products and 70 were polymorphic with the allele number range of 3-16 alleles/locus (average 7.0 alleles/locus). When EST database mining was performed, 66 microsatellites containing ESTs were identified from 3467 sequences deposited in GenBank. Based on cluster analysis of length and GC content of the flanking regions, 47 primer pairs were designed and 23 scorable EST SSRs were obtained. Compared with genomic SSRs developed in this study, EST SSRs showed lower genetic variability with an average of 4.2 alleles/locus. The results in the present study demonstrate that modified FIASCO-colony hybridization is an efficient and low-cost method for the isolation of large numbers of microsatellite markers for scallop species.

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“…Several ecological and life history patterns and their relevance to our program are addressed in more detail elsewhere in this volume Eggleston et al, 2008;Hines et al, 2008;Lipcius et al, 2008;Seitz et al, 2008;Young et al, 2008) and other peer-reviewed literature Steven et al, 2003Steven et al, , 2005. With respect to the economic feasibility of the program, it is very clear that understanding blue crab biology is essential to reducing the production cost of cultured juveniles.…”

Section: Basic Studies Of Blue Crab Biologymentioning

confidence: 99%

The Chesapeake Bay Blue Crab (Callinectes sapidus): A Multidisciplinary Approach to Responsible Stock Replenishment

Zohar

Hines

Zmora

et al. 2008

Reviews in Fisheries Science

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Genetic markers in blue crabs (Callinectes sapidus) I: Isolation and characterization of microsatellite markers

Cited by 14 publications

References 23 publications

High Connectivity among Blue Crab (Callinectes sapidus) Populations in the Western South Atlantic

High Connectivity among Blue Crab (Callinectes sapidus) Populations in the Western South Atlantic

Characterization of 95 novel microsatellite markers for Zhikong scallop Chlamys farreri using FIASCO-colony hybridization and EST database mining

The Chesapeake Bay Blue Crab (Callinectes sapidus): A Multidisciplinary Approach to Responsible Stock Replenishment

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