Genetic Interactions between Phytochrome A, Phytochrome B, and Cryptochrome 1 during Arabidopsis Development1

Neff, Michael M.; Chory, Joanne

doi:10.1104/pp.118.1.27

Cited by 477 publications

(500 citation statements)

References 36 publications

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“…S1). Reduced anthocyanin levels in CRY1-deficient Arabidopsis seedlings have been shown in continuous blue light (Ahmad et al, 1995;Lin et al, 1996) and in continuous white light (Neff and Chory, 1998). However, our results demonstrate that CRY1 and HY5 also play a key role in the high irradiance-induced accumulation of anthocyanin.…”

Section: The Cry1 and Hy5 Mutants Demonstrate A Defective Stress Respcontrasting

confidence: 53%

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Genome-Wide Gene Expression Analysis Reveals a Critical Role for CRYPTOCHROME1 in the Response of Arabidopsis to High Irradiance

et al. 2007

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Exposure to high irradiance results in dramatic changes in nuclear gene expression in plants. However, little is known about the mechanisms by which changes in irradiance are sensed and how the information is transduced to the nucleus to initiate the genetic response. To investigate whether the photoreceptors are involved in the response to high irradiance, we analyzed expression of EARLY LIGHT-INDUCIBLE PROTEIN1 (ELIP1), ELIP2, ASCORBATE PEROXIDASE2 (APX2), and LIGHT-HARVESTING CHLOROPHYLL A/B-BINDING PROTEIN2.4 (LHCB2.4) in the phytochrome A (phyA), phyB, cryptochrome1 (cry1), and cry2 photoreceptor mutants and long hypocotyl5 (hy5) and HY5 homolog (hyh) transcription factor mutants. Following exposure to high intensity white light for 3 h (1,000 mmol quanta m 22 s 21 ) expression of ELIP1/2 and APX2 was strongly induced and LHCB2.4 expression repressed in wild type. The cry1 and hy5 mutants showed specific misregulation of ELIP1/2, and we show that the induction of ELIP1/2 expression is mediated via CRY1 in a blue light intensity-dependent manner. Furthermore, using the Affymetrix Arabidopsis (Arabidopsis thaliana) 24 K Gene-Chip, we showed that 77 of the high lightresponsive genes are regulated via CRY1, and 26 of those genes were also HY5 dependent. As a consequence of the misregulation of these genes, the cry1 mutant displayed a high irradiance-sensitive phenotype with significant photoinactivation of photosystem II, indicated by reduced maximal fluorescence ratio. Thus, we describe a novel function of CRY1 in mediating plant responses to high irradiances that is essential to the induction of photoprotective mechanisms. This indicates that high irradiance can be sensed in a chloroplast-independent manner by a cytosolic/nucleic component.

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Section: The Cry1 and Hy5 Mutants Demonstrate A Defective Stress Respcontrasting

confidence: 53%

“…Relative anthocyanin levels were determined according to Neff and Chory (1998). In brief, 50 to 70 mg seedlings were incubated overnight in 450 mL methanol acidified with 1% HCl.…”

Section: Analysis Of Anthocyanin Contentmentioning

confidence: 99%

Genome-Wide Gene Expression Analysis Reveals a Critical Role for CRYPTOCHROME1 in the Response of Arabidopsis to High Irradiance

et al. 2007

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“…The ability of white light to inhibit hypocotyl growth is reduced in phytochrome A (phyA), phyB, and phyAphyB mutants (Neff and Chory, 1998). When these phy mutants were transformed with APY1:GUS and APY2: GUS, GUS expression was detected in the hypocotyls of light-grown seedlings (Fig.…”

Section: Light Down-regulates Apyrase Expression In Parallel With Gromentioning

confidence: 99%

Apyrases (Nucleoside Triphosphate-Diphosphohydrolases) Play a Key Role in Growth Control in Arabidopsis

et al. 2007

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Expression of two Arabidopsis (Arabidopsis thaliana) apyrase (nucleoside triphosphate-diphosphohydrolase) genes with high similarity, APY1 and APY2, was analyzed during seedling development and under different light treatments using b-glucuronidase fusion constructs with the promoters of both genes. As evaluated by b-glucuronidase staining and independently confirmed by other methods, the highest expression of both apyrases was in rapidly growing tissues and/or tissues that accumulate high auxin levels. Red-light treatment of etiolated seedlings suppressed the protein and message level of both apyrases at least as rapidly as it inhibited hypocotyl growth. Adult apy1 and apy2 single mutants had near-normal growth, but apy1apy2 doubleknockout plants were dwarf, due primarily to reduced cell elongation. Pollen tubes and etiolated hypocotyls overexpressing an apyrase had faster growth rates than wild-type plants. Growing pollen tubes released ATP into the growth medium and suppression of apyrase activity by antiapyrase antibodies or by inhibitors simultaneously increased medium ATP levels and inhibited pollen tube growth. These results imply that APY1 and APY2, like their homologs in animals, act to reduce the concentration of extracellular nucleotides, and that this function is important for the regulation of growth in Arabidopsis.

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“…The fact that overexpression of Arabidopsis CRY1 in transgenic tobacco also resulted in a short hypocotyl phenotype suggests that the cry1 signaling process is conserved in different plant species. Complex functional interactions of cry1 with phytochromes have been analyzed in a number of studies using Arabidopsis or tomato mutant lines impaired in both types of photoreceptors (Casal and Boccalandro, 1995;Ahmad and Cashmore, 1997;Ahmad et al, 1998b;Casal and Mazzella, 1998;Neff and Chory, 1998;Wang and Iino, 1998;Hennig et al, 1999aHennig et al, , 1999bFolta and Spalding, 2001a;Weller et al, 2001).…”

Section: Function Of Cryptochromes In De-etiolationmentioning

confidence: 99%

Blue Light Receptors and Signal Transduction

2002

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Genetic Interactions between Phytochrome A, Phytochrome B, and Cryptochrome 1 during Arabidopsis Development1

Cited by 477 publications

References 36 publications

Genome-Wide Gene Expression Analysis Reveals a Critical Role for CRYPTOCHROME1 in the Response of Arabidopsis to High Irradiance

Genome-Wide Gene Expression Analysis Reveals a Critical Role for CRYPTOCHROME1 in the Response of Arabidopsis to High Irradiance

Apyrases (Nucleoside Triphosphate-Diphosphohydrolases) Play a Key Role in Growth Control in Arabidopsis

Blue Light Receptors and Signal Transduction

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