2019
DOI: 10.1101/757773
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Abstract: 10Stress response is mediated by transcription of stress responsive genes. F-box motif protein 11 Saf1 involves in SCF-E3 ligase mediated degradation of the adenine deaminase, Aah1 upon 12 nutrient stress. Four transcription regulators, BUR6, MED6, SPT10, SUA7, have been 13 reported for SAF1 gene in genome database of Saccharomyces cerevisiae. Here in this study 14 an in-silco analysis of gene expression and transcription factor databases was carried out to 15 understand the regulation of SAF1 gene expre… Show more

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Cited by 2 publications
(10 citation statements)
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“…The list of strains used for the study is provided in Table 1. Deletion of ORFs was conducted as per protocols [25,26]. For ORF deletion, a selectable marker cassette with 40 bp homology to either end of the target ORF was a polymerase chain reaction generated from plasmids (Table 2), using Kapa Taq Polymerase (Sigma‐Aldrich).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The list of strains used for the study is provided in Table 1. Deletion of ORFs was conducted as per protocols [25,26]. For ORF deletion, a selectable marker cassette with 40 bp homology to either end of the target ORF was a polymerase chain reaction generated from plasmids (Table 2), using Kapa Taq Polymerase (Sigma‐Aldrich).…”
Section: Methodsmentioning
confidence: 99%
“…Semiquantitative spot assay [26] was performed for cellular growth response of each strain in the presence of alternate carbon source and stress agents on the YPD solid media. Yeast cells were grown in YPD liquid medium at 30°C, with 200‐rpm shaking until log phase was attained.…”
Section: Methodsmentioning
confidence: 99%
“…Comparative growth assessment of WT and mutant strains on YPD solid and liquid media was carried as mentioned in the cited reports. [35,36] Single colony of each strain was streaked on a YPD agar plate followed by incubation at 30°C for 2-3 days. For comparative growth assessment in liquid broth, each strain with starting optical density (OD) 600 nm as 0.05 was grown in triplicates, for 14 hrs and OD was measured after every 2 hrs intervals at 600 nm using UV-800 spectrophotometer.…”
Section: Growth Assaymentioning
confidence: 99%
“…For a comparative assessment of the morphological features between WT and mutant strains, the method mentioned in the literature was adopted. [34,36] Each strain was grown to log phase in YPD medium at 30°C, harvested by centrifugation, and imaged using phase filters under Leica DM3000 microscope under ×100 objective lens. [36] For estimation of cell size of WT and mutants, the method mentioned [37] was adopted.…”
Section: Microscopic Assay and Cell Size Determinationmentioning
confidence: 99%
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