Genetic Improvement of Grain Quality Promoted by High and New Technology in Rice

Peng, Bo; Li, Jun; Kong, Dongyan; He, Lulu; Li, Mengge; Nassirou, Tondi-Yacouba; Yu, Peng; Su, Xiaohua; Peng, Juan; Jiang, Yue; Sun, Yan; Rui, Pang; Xin, Qingqing; Liu, Yuchen; Guo, Gui-Ying; Li, Jin-Tiao; Wang, Quanxiu; Duan, Bin; Yuan, Hongyu

doi:10.5539/jas.v11n1p81

Cited by 9 publications

(8 citation statements)

References 44 publications

(53 reference statements)

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“…DNA targeted endonuclease technology can repair damaged DNA by homologous recombination or non homologous terminal connection by breaking double strands of DNA at the target site of genome, so as to achieve targeted knockout, knockin and gene modification of target site (Peng et al, 2019). The immune memory of CRISPR/Cas system is stored in the form of foreign genome interval sequence inserted into CRISPR array (Koonin et al, 2017).…”

Section: Crispr/cas9 Technologymentioning

confidence: 99%

“…It is urgent to further improve the yield of rice to ensure global food security (He, 2014). In recent decades, with the continuous improvement of people's living standards, the demand for high-quality rice is also increasing (Peng et al, 2019). Therefore, increasing rice yield and improving rice quality are the focus of attention of rice breeders and botanists.…”

Section: Introductionmentioning

confidence: 99%

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Application of CRISPR/Cas9 Technology in the Genetic Improvement of Rice Yield and Quality Characters

Peng¹,

Ma²,

Yang³

et al. 2020

JBLS

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Rice (Oryza sativa L.) is one of the most important food crops in the world. With the continuous increase of global population and the continuous improvement of people's living standards, it has become the focus of attention of rice breeders and botanists to improve the yield and quality of rice. In the new genome editing technology, the CRISPR/Cas system can accurately and specifically edit the target genome region. In recent years, CRISPR/Cas9 technology has made important progress in studying the function of new genes, increasing rice yield and improving rice quality. Therefore, this paper mainly reviewed the application of CRISPR/Cas9 technology in the genetic improvement of rice yield and rice quality in recent years, and looked forward to the application prospect of CRISPR/Cas9 technology in rice genetic breeding, which will provide important reference for the cultivation of new high-quality and high-yield rice varieties.

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Section: Crispr/cas9 Technologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of CRISPR/Cas9 Technology in the Genetic Improvement of Rice Yield and Quality Characters

Peng¹,

Ma²,

Yang³

et al. 2020

JBLS

Self Cite

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“…The cultivated species are Oryza sativa (Asian rice), grown worldwide, and the second species is Oryza glaberrima (African rice), cultivated on a limited scale in the west. Rice comes in second after wheat in its nutritional content, as it is rich in high-energy sources such as carbohydrates and proteins [1,2]. The rice crop has different morphological and physiological IOP Publishing doi:10.1088/1755-1315/1262/5/052039 2 characteristics from other grains, where the stomata are large, the skin of its leaves is very thin, and it is relatively resistant to water loss, its carbon metabolism rate is lower, and the water use efficiency is lower.…”

Section: Introductionmentioning

confidence: 99%

Induction of Callus of Two Cultivars from Rice Seed Embryos by the Effect of Abiotic Factors for Drought Tolerance ex Vivo

Najm,

Hamza

2023

IOP Conf. Ser.: Earth Environ. Sci.

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The experiment was conducted at the Plant Tissue Culture Laboratory, College of Agricultural Engineering Sciences, University of Baghdad, during the period 2021-2023, with the aim of inducing Callus from embryos of two rice cultivars: black rice and Amber 33, to enhance drought tolerance in vitro. The Completely Randomized Design (CRD) was applied for experimental arrangements. Different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) (0, 1.5, 3, 4.5 mg L-1) were used to stimulate Callus, along with UV radiation of type UV-C with a wavelength (<280 nanometers), It does not affect the environment because it is absorbed by the atmosphere’s oxygen and ozone, for various durations of 15, 30, 45, and 60 minutes. A control treatment was also used for comparison. The experimental stress conditions were induced using different concentrations (0, 1.5, 3, 4.5, and 6%) of polyethylene glycol (PEG). The fresh and dry weights of Callus were measured for all treatments, in addition to assessing the drought tolerance index. The results showed significant differences for most of the studied traits. The highest fresh and dry weight of Callus was achieved at a concentration of 3 mg L-1 of 2,4-D, at 258.9 mg and 26.06 mg, respectively. The control treatment with UV-C radiation outperformed with the highest fresh and dry weight of Callus, reaching 356.8 mg and 37.81 mg, respectively, compared to other irradiation doses. The control treatment with other PEG concentrations yielded the highest fresh and dry weight of Callus for the cultivars treated with UV-C radiation, reaching 356.4 mg and 38.81 mg, respectively. The drought tolerance index in terms of dry weight, the treatment with 1.5% PEG yielded the highest rate at 0.73 mg, while the treatment with 6% PEG resulted in the lowest rate at 0.21 mg. additionally; the black rice cultivar treated with UV-C radiation outperformed the Amber 33 rice cultivar in terms of both fresh and dry weights of Callus.

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“…Studies have shown that through CRISPR/Cas9-mediated genome editing, the probability of obtaining homozygous or biallelic genes is as high as 90% in T 0 generation [ 24 ]. Previous studies suggest that mutations in the GS3 gene results in increased GL and GWT, and the overall yield improvement [ 25 ]. Zhao et al (2018) [ 16 ] used CRISPR/Cas9 technology to generate mutations in the first exon of GS9 , which resulted in a frameshift mutation, disrupted GS9 normal expression, and increased GL.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9 Guided Mutagenesis of Grain Size 3 Confers Increased Rice (Oryza sativa L.) Grain Length by Regulating Cysteine Proteinase Inhibitor and Ubiquitin-Related Proteins

Usman

Zhao

Nawaz

et al. 2021

IJMS

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Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas9)-mediated genome editing has become an important way for molecular breeding in crop plants. To promote rice breeding, we edited the Grain Size 3 (GS3) gene for obtaining valuable and stable long-grain rice mutants. Furthermore, isobaric tags for the relative and absolute quantitation (iTRAQ)-based proteomic method were applied to determine the proteome-wide changes in the GS3 mutants compared with wild type (WT). Two target sites were designed to construct the vector, and the Agrobacterium-mediated method was used for rice transformation. Specific mutations were successfully introduced, and the grain length (GL) and 1000-grain weight (GWT) of the mutants were increased by 31.39% and 27.15%, respectively, compared with WT. The iTRAQ-based proteomic analysis revealed that a total of 31 proteins were differentially expressed in the GS3 mutants, including 20 up-regulated and 11 down-regulated proteins. Results showed that differentially expressed proteins (DEPs) were mainly related to cysteine synthase, cysteine proteinase inhibitor, vacuolar protein sorting-associated, ubiquitin, and DNA ligase. Furthermore, functional analysis revealed that DEPs were mostly enriched in cellular process, metabolic process, binding, transmembrane, structural, and catalytic activities. Pathway enrichment analysis revealed that DEPs were mainly involved in lipid metabolism and oxylipin biosynthesis. The protein-to-protein interaction (PPI) network found that proteins related to DNA damage-binding, ubiquitin-40S ribosomal, and cysteine proteinase inhibitor showed a higher degree of interaction. The homozygous mutant lines featured by stable inheritance and long-grain phenotype were obtained by using the CRISPR/Cas9 system. This study provides a convenient and effective way of improving grain yield, which could significantly accelerate the breeding process of long-grain japonica parents and promote the development of high yielding rice.

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Genetic Improvement of Grain Quality Promoted by High and New Technology in Rice

Cited by 9 publications

References 44 publications

Application of CRISPR/Cas9 Technology in the Genetic Improvement of Rice Yield and Quality Characters

Application of CRISPR/Cas9 Technology in the Genetic Improvement of Rice Yield and Quality Characters

Induction of Callus of Two Cultivars from Rice Seed Embryos by the Effect of Abiotic Factors for Drought Tolerance ex Vivo

CRISPR/Cas9 Guided Mutagenesis of Grain Size 3 Confers Increased Rice (Oryza sativa L.) Grain Length by Regulating Cysteine Proteinase Inhibitor and Ubiquitin-Related Proteins

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