Genetic impacts induced by BaP and Pb in Mytilus coruscus: Can RAPD be a validated tool in genotoxicity evaluation both in vivo and in vitro?

Qu, Moyuan; Ding, Jiawei; Wang, Yi; Chen, Siyu; Zhang, Yifei; Di, Yanan

doi:10.1016/j.ecoenv.2018.11.066

Cited by 12 publications

(4 citation statements)

References 54 publications

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“…RAPD is a technique for detecting polymorphisms at the molecular level of DNA, which was often used to detect the effects of metal elements, chemicals, and drugs on the genetic material of animals and plants [13][14][15]. It was also used to determine the genotoxicity of environmental pollutants and some carcinogens in aquatic animals [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis

Yin

Feng

et al. 2022

BioMed Research International

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Objective. To investigate the genotoxicity of metformin on planarian with different concentrations and exposure times. Methods. The planarians were treated, respectively, with 10 mmol/L and 50 mmol/L metformin for 1, 3, and 5 days, and then, the comet assay and random amplified polymorphic DNA (RAPD) analysis were performed. 13 random primers were used for PCR amplification with the genomic DNAs as templates. Planarians cultured in clear water were used as the control. Genomic template stability (GTS) was calculated by comparing and analyzing the RAPD patterns of the control group and the treatment groups. Results. In the comet assay, DNA damage of planarians treated with 10 mmol/L metformin for 1, 3, and 5 days was 10.2%, 25.4%, and 36.8%, respectively, and that of planarians treated with 50 mmol/L metformin was 40.6%, 62.8%, and 65.4%, respectively. GTS values of planarians exposed to 10 mmol/L metformin for 1, 3, and 5 days were 64.1%, 62.8%, and 52.6%, respectively, and those of planarians exposed to 50 mmol/L metformin for 1, 3, and 5 days were 52.6%, 51.3%, and 50%, respectively. DNA damage increased and GTS values decreased with the increasing metformin exposure concentration and exposure time. Conclusion. Metformin has certain genotoxicity on planarian in a dose- and time-related manner. The comet assay and RAPD analysis are highly sensitive methods for detecting genotoxicity with drugs.

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Section: Introductionmentioning

confidence: 99%

Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis

Yin

Feng

et al. 2022

BioMed Research International

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“…Due to their persistence, semivolatility, easy accumulation and potential biomagnification in organisms, pollution from PAHs is a cause Nanomaterials 2021, 11, 1309 2 of 18 of great concern [2,3]. Among them, benzo(a)pyrene, (B(a)P) a highly lipophilic compound, is considered to be representative of the class of PAHs in organic pollutants [4] such as petroleum and crude oils. B(a)P is commonly detected in the marine environment where it comes in contact with biota, because of either acute or chronic exposure, exerting toxicity at different biological levels, resulting in consequences such as reproductive dysfunction, immune system destruction, neuro and endocrine toxicity, mutagenic response, tumor induction [3,5,6].…”

Section: Introductionmentioning

confidence: 99%

Suitability of Nanoparticles to Face Benzo(a)pyrene-Induced Genetic and Chromosomal Damage in M. galloprovincialis. An In Vitro Approach

et al. 2021

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Benzo(a)pyrene (B(a)P) is a well-known genotoxic agent, the removal of which from environmental matrices is mandatory, necessitating the application of cleaning strategies that are harmless to human and environmental health. The potential application of nanoparticles (NPs) in the remediation of polluted environments is of increasing interest. Here, specifically designed NPs were selected as being non-genotoxic and able to interact with B(a)P, in order to address the genetic and chromosomal damage it produces. A newly formulated pure anatase nano-titanium (nano-TiO2), a commercial mixture of rutile and anatase, and carbon black-derived hydrophilic NPs (HNP) were applied. Once it had been ascertained that the NPs selected for the work did not induce genotoxicity, marine mussel gill biopsies were exposed in vitro to B(a)P (2 μg/mL), alone and in combination with the selected NPs (50 µg/mL nano-TiO2, 10 µg/mL HNP). DNA primary reversible damage was evaluated by means of the Comet assay. Chromosomal persistent damage was assessed on the basis of micronuclei frequency and nuclear abnormalities by means of the Micronucleus-Cytome assay. Transmission Electron Microscopy (TEM) was performed to investigate the mechanism of action exerted by NPs. Pure Anatase n-TiO2 was found to be the most suitable for our purpose, as it is cyto- and genotoxicity free and able to reduce the genetic and chromosomal damage associated with exposure to B(a)P.

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“…Gill cells were selected for this study since they were easy to obtain, and in nature, they come into contact with relatively large volumes of seawater compared to the rest of the animal (average filtering rate for an adult mussel is approximately 2 l/h at 20 o C), thus conferring them with the potential for being a suitable target tissue for mutagen exposure (Dixon & Clarke, 1982;Venier & Canova, 1996;Pearson et al, 2018;Qu et al, 2019). The gills are initial sites of uptake TBT by M. edulis (Laughlin et al, 1986), and accumulation of TBT in gills and viscera is greater than in other parts of the bivalve mussels (Huang & Wang, 1995).…”

Section: Cell Preparationmentioning

confidence: 99%

DNA damage and shell malformation in Blue Mussel, <i>Mytilus edulis</i>

Yaqin

2022

Akuatikisle: J. Aqua. Coast. & Isle

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Pollutants in waters that cause DNA damage, such as tributyltin, have been known to cause malformations in the mussel shell, which need attention to be studied. Shell malformations or deformities of blue mussels were observed in Norsminde Fjord harbour. The mean shell thickness index of blue mussels from the polluted site was greater than it from expected clean waters. DNA damages detected using the versatile comet assay which expressed as tail length and olive tail moment were evaluated in blue mussels gill cells both in situ and in vitro. The results showed that the average of DNA damages in treatments, namely the polluted site, tributyltin chloride (TBTC) (10 g/l), and hydrogen peroxide (H2O2) (10 M) were different from the expected unpolluted site. Although comet assay is considered as an unspecific assay, the results proposed the similarity of DNA damage character of blue mussel gill cells when exposed to tributyltin chloride and taken directly from the polluted site. The data obtained are important for assessing the environmental risks created by genotoxic agents, e.g. tributyltin, used as antifouling agents in marine paints.

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Genetic impacts induced by BaP and Pb in Mytilus coruscus: Can RAPD be a validated tool in genotoxicity evaluation both in vivo and in vitro?

Cited by 12 publications

References 54 publications

Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis

Genotoxicity Evaluation of Metformin in Freshwater Planarian Dugesia japonica by the Comet Assay and RAPD Analysis

Suitability of Nanoparticles to Face Benzo(a)pyrene-Induced Genetic and Chromosomal Damage in M. galloprovincialis. An In Vitro Approach

DNA damage and shell malformation in Blue Mussel, <i>Mytilus edulis</i>

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