Genetic identification of two mudskipper species (Pisces: Gobiidae) from Bogowonto Lagoon (Yogyakarta, Indonesia) using COI mitochondrial gene as a DNA barcoding marker

Arisuryanti, Tuty; Hasan, Rika Lathif; Koentjana, Johan Putra

doi:10.1063/1.5050164

Cited by 11 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dahruddin et al (2017) identified three species of members of the genus Periophthalmus namely P. novemradiatus, P.kalolo, and P. argentilineatus in Java (Pandeglang, Cilacap, Tulung Agung) and Bali (Jembrana). Furthermore, Arisuryanti et al (2018) successfully identified two species namely P. argentilineatus and P. kalolo in Bogowonto Lagoon (Yogyakarta). This study also revealed that P. argentilineatus from Bogowonto Lagoon has a close genetic relationship with P. argentilineatus from Pandeglang.…”

Section: Introductionmentioning

confidence: 99%

DNA Barcode of Barred Mudskipper (Periophthalmus argentilineatus Valenciennes, 1837) from Tekolok Estuary (West Nusa Tenggara, Indonesia) and Their Phylogenetic Relationship with Other Indonesian Barred Mudskippers

Rha’ifa

Audrea

Hakim

et al. 2021

J.Tropical Biodiversity Biotechnology

Self Cite

View full text Add to dashboard Cite

Barred mudskipper (Periophthalmus argentilineatus) has a potency to be developed as protein for human consumption and ornamental fish. The fish also has an important role in mangrove ecosystems. Nevertheless, many barred mudskippers have been considered a cryptic species. Therefore, accurate identification is needed to clarify species identification of the barred mudskipper using DNA barcoding. This research aimed to identify barred mudskippers from Tekolok Estuary (East Lombok, West Nusa Tenggara, Indonesia) using COI mitochondrial gene as a DNA barcode and analyze genetic relationship with other barred mudskippers from several regions of Indonesia recorded in GenBank. This study used a PCR method with universal primers FishF2 and FishR2. The data was then analysed using DNASTAR, BLAST, Mesquite, MEGA, DnaSP, BEAST, GenAlEx, and NETWORK. The results revealed that barred mudskipper from Tekolok Estuary has been verified as Periophthalmus argentilineatus. The results also exhibited that P. argentilineatus from Tekolok Estuary has a close genetic relationship to P.argentilineatus from Tukad Bilukpoh (Jembrana, Bali). In addition, phylogenetic analysis showed that P.argentilineatus from Indonesia consisted of two clades with a genetic distance of approximately 6.64%. This analysis revealed evidence of the cryptic diversity of P.argentilineatus from Indonesia. Further detailed studies are needed to clarify whether Indonesian P.argentilineatus should be categorized into more than one species or single species with several subspecies.

show abstract

Section: Introductionmentioning

confidence: 99%

DNA Barcode of Barred Mudskipper (Periophthalmus argentilineatus Valenciennes, 1837) from Tekolok Estuary (West Nusa Tenggara, Indonesia) and Their Phylogenetic Relationship with Other Indonesian Barred Mudskippers

Rha’ifa

Audrea

Hakim

et al. 2021

J.Tropical Biodiversity Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Keith et al (2005) also reported that the Sicyopterus lagocephalus from different distant archipelagos has minimal genetic distances and suggests they as the same species. The study by Arisuryanti et al (2018) showed that Periophthalmus argentilineatus in Bogowonto Lagoon (Middle Java) and population in Pandeglang (West Java) clustered together with low sequence divergences. H. compressa, S. lagocephalus, and P. argentilineatus are the species from other Gobies.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of transparent gobies in three Sumatran lakes, inferred from mitochondrial Cytochrome Oxidase I (COI) gene

2019

View full text Add to dashboard Cite

Abstract. Roesma DI, Tjong DH, Aidil DR. 2020. Phylogenetic analysis of transparent gobies in three Sumatran lakes, inferred from mitochondrial Cytochrome Oxidase I (COI) gene. Biodiversitas 21: 43-48. The transparent gobies fish found in three lakes in Sumatra island is known as Rinuak fish (in Maninjau Lake and Singkarak Lake, West Sumatra, Indonesia) or Badar fish (in Siais Lake, North Sumatra, Indonesia), and are morphologically very similar to the Gobiopterus brachypterus. The phylogenetic study was carried out by analyzing 619 base pairs of the mitochondrial DNA cytochrome oxidase subunit I (COI) gene in 12 fish individuals from the three lakes. Rinuak and Badar fish in three populations have four haplotypes. The sequence divergences in and between populations are very low (0.0-0.5%). This value indicates that Rinuak and Badar fish are the same species with low genetic diversity. The phylogenetic tree illustrates that this fish belongs to the group of Gobiidae and a sister taxon from G. brachypterus.

show abstract

“…Previous studies showed that cryptic species has occurred in genus Elacatinus, Tigrigobius, Trimma, Eviota using COI mitochondrial gene (Victor 2010;Victor 2014;Winterbottom et al 2014;Tornabene et al 2015), genus Ponticola using Cyt-b mitochondrial gene (Vasil'eva et al 2016) and genus Mugilogobius using three mitochondrial markers (ND5, Cytb, and D-loop) (Huang et al 2016). Moreover, cryptic species are also observed in mudskippers Boleophthalmus pectinirostris from the western Pacific coast of East Asia and the Strait of Malacca in Malaysia using Mitochondrial ND5 gene and nuclear RAG-1 gene (Chen et al 2014), Periophthalmus argentilineatus from East-African and Indo-Malaya using two mtDNA markers (D-loop and 16S rDNA) and one nDNA region (RAG-1) (Polgar et al 2014), and Periophthalmus argentilineatus from Bogowonto Lagoon, Yogyakarta, Indonesia using COI mitochondrial gene as a DNA barcoding marker (Arisuryanti et al 2018). Since cryptic species are morphologically similar, distinguishing them solely on morphological characteristics is nearly impossible.…”

Section: Introductionmentioning

confidence: 91%

Molecular Identification of Mudskipper Fish (Periophthalmus spp.) from Baros Beach, Bantul, Yogyakarta

Aji

Arisuryanti

2021

J.Tropical Biodiversity Biotechnology

Self Cite

View full text Add to dashboard Cite

Mudskipper fish is amphibious fish belonging to the family Gobiidae. Coastal communities widely consume mudskipper to meet their animal protein needs. Mudskipper is primarily cryptic species that are morphologically difficult to identify and distinguish from other mudskipper fish species. Consequently, it can be confused with the naming of mudskipper fish species and can affect the conservation efforts of the fish in their habitat. One of the molecular approaches that can be used to identify the fish species quickly and accurately is DNA barcoding using the COI mitochondrial gene. However, the research on the identification of mudskipper fish in Indonesia is still very limited. Therefore, this study aimed to identify 26 mudskipper fish from Baros Beach, Bantul, Yogyakarta, using COI mitochondrial gene as a molecular marker for DNA barcoding. The method used in this study was a PCR method with universal primers, FishF2 and FishR2. The data obtained were then analyzed using GeneStudio, DNASTAR, BLAST, Identification Engine, Mesquite, MEGAX, and BEAST. The analysis was conducted to obtain similarity, genetic distance and reconstruct a phylogenetic tree. The result revealed that all 26 samples of mudskippers collected from Baros Beach were identified in one genus, namely Periophthalmus, and consisted of 3 species, namely P. kalolo (16 samples), P. argentilineatus (9 samples), and P. novemradiatus (1 sample). Furthermore, this study also discovered a suspected cryptic species in P. argentilineatus with a genetic distance of 5.46-5.96% between clade E, F compared with clade G. Further morphological studies are needed to confirm the species status of these three clades before solidly proclaim that they are cryptic species.

show abstract

Genetic identification of two mudskipper species (Pisces: Gobiidae) from Bogowonto Lagoon (Yogyakarta, Indonesia) using COI mitochondrial gene as a DNA barcoding marker

Cited by 11 publications

References 17 publications

DNA Barcode of Barred Mudskipper (<i>Periophthalmus argentilineatus</i> Valenciennes, 1837) from Tekolok Estuary (West Nusa Tenggara, Indonesia) and Their Phylogenetic Relationship with Other Indonesian Barred Mudskippers

DNA Barcode of Barred Mudskipper (<i>Periophthalmus argentilineatus</i> Valenciennes, 1837) from Tekolok Estuary (West Nusa Tenggara, Indonesia) and Their Phylogenetic Relationship with Other Indonesian Barred Mudskippers

Phylogenetic analysis of transparent gobies in three Sumatran lakes, inferred from mitochondrial Cytochrome Oxidase I (COI) gene

Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta

Contact Info

Product

Resources

About