Genetic homogeneity in the commercial pink shrimp Farfantepenaeus paulensis revealed by COI barcoding gene

Abstract

a b s t r a c tThe pink shrimp Farfantepenaeus paulensis is one of the most commercially exploited species in Brazil's South and Southeastern regions. Specific information about the status of its genetic variation is necessary to promote more effective management procedures. The genetic variation of the population of F. paulensis was investigated in five localities along southern and southeastern coast of Brazil. Sampling was performed with a commercial fishing boat. Total genomic DNA was extracted from abdominal muscle tissues and was used to DNA amplification by PCR. The COI gene was used as a DNA barcoding marker. The 570 bp COI gene sequences were obtained from all 45 individuals. The haplotype network showed no genetic variability among the population stocks, which was confirmed by Molecular Variance Analysis. The final alignment showed that inside species there is haplotype sharing among the sampled localities, since one haplotype is shared by 38 individuals belonging to all the five sampled regions, with no biogeographic pattern. This result is reasonable since there are no geographical barriers or habitat disjunction that might serve as a barrier to gene flow among the sampled localities. Possible reasons and consequences of the genetic homogeneity found are discussed. The results complement ecological studies concerning the offseason: since it is a single stock, the same protection strategy can be applied. However, the genetic homogeneity found in this study combined with the intensive fishery effort and the species biology can result in severe consequences for the F. paulensis.

Discordance in the identification of juvenile pink shrimp ( Farfantepenaeus brasiliensis and F. paulensis : Family Penaeidae): An integrative approach using morphology, morphometry and barcoding

“…Such result is reasonable since there are no geographical barriers or habitat disjunction that might serve as a barrier to gene flow among sampled localities. The possible consequences for this homogeneity were recently discussed in Teodoro et al (2015).…”

“…Tissue extraction, PCR amplification with specific primers, product cleanup, and sequencing were conducted following our laboratory protocols (Mantelatto et al, 2007(Mantelatto et al, , 2009, with specific modifications (Teodoro et al, 2015). Sequence editing was performed using the BioEdit program, version 7.0.9 (Hall, 1999).…”

Variabilidade populacional em manguezais: análises moleculares e morfológicas em caranguejos Brachyura (Crustacea: Decapoda)

“…Uma vez que esses efeitos podem influenciar a distribuição da variação genética entre e dentro da população, os efeitos genéticos da redução de populações em tamanho têm sido bastante estudados (Wright, 1931;Nei et al, 1975). Alguns estudos, inclusive, têm diagnosticado espécies exibindo elevada homogeneidade genética, caracterizando uma consequência do efeito gargalo populacional (e.g., Carvalho-Batista et al, 2014;Teodoro et al, 2015). Essa homogeneização genética combinada com uma distribuição restrita e limitada pode tornar as espécies mais sensíveis a mudanças consequentes de ações antrópicas (Teodoro et al, 2015).…”

“…Alguns estudos, inclusive, têm diagnosticado espécies exibindo elevada homogeneidade genética, caracterizando uma consequência do efeito gargalo populacional (e.g., Carvalho-Batista et al, 2014;Teodoro et al, 2015). Essa homogeneização genética combinada com uma distribuição restrita e limitada pode tornar as espécies mais sensíveis a mudanças consequentes de ações antrópicas (Teodoro et al, 2015).…”

“…Assim, a compreensão das mudanças genéticas e das respostas evolutivas em populações de espécies exploradas é determinante no estabelecimento de manejos e exploração sustentável dos recursos biológicos (Walsh et al, 2006;Teodoro et al, 2015). BIBLIOGRAFIA…”