Genetic engineering of polyamine and carbohydrate metabolism for osmotic stress tolerance in higher plants

Rajam, Manchikatla Venkat; Dagar, Saloni; Waie, B.; Yadav, J. S.; Kumar, Pankaj; Shoeb, F.; Kumria, R.

doi:10.1007/bf02936141

Cited by 31 publications

(14 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plant transformation is an essential tool, both for the experimental investigation of gene function and for the improvement of crops either by enhancing existing traits or introducing new genes, and the genetic engineering of biosynthetic pathways associated with stress responses has emerged as a promising approach to improve tolerance in crops. Adverse environmental conditions stimulate plants to employ various mechanisms, such as shifts in physiology and the increased expression of stress-associated genes leading to the formation of a wide variety of low-molecular-weight metabolites, notably osmolytes and proteins, to cope with the stress condition (Rajam et al 1998;Zhu 2001;Prabhavathi et al 2002). These osmolytes include fructans, trehalose, mannitol, myo-inositol, proline, glycine-betaine and polyamines, which accumulate in significant amounts and may be helpful in maintaining osmotic potential, ionic balance, membrane integrity and oxygen free radical level and in protecting chromatin under these conditions (Rajam et al 1998;Bohnert and Shen 1999;Prabhavathi et al 2002).…”

Section: Introductionmentioning

confidence: 96%

Transgenic tomato cv. Pusa Uphar expressing a bacterial mannitol-1-phosphate dehydrogenase gene confers abiotic stress tolerance

Khare

Goyary

Singh

et al. 2010

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

A bacterial mannitol-1-phosphate dehydrogenase (mtlD) gene driven by the constitutive cauliflower mosaic virus (CaMV) 35S promoter was transferred into tomato plants using an Agrobacterium tumefaciens-mediated transformation protocol in an attempt to improve abiotic stress tolerance in the transformed plants. Transgene integration was confirmed by PCR analysis and Southern blot analysis, and transgene expression was confirmed by reverse transcription (RT)-PCR and direct mtlD (EC 1.1.1.17) activity. Upon exposure to low temperature stress (4°C) in a cold chamber, transgenic plants survived up to 48 h, while nontransformed plants were unable to survive and gradually died. Transgenic plants subjected to the chilling stress showed a significant decrease in electrolyte leakage and increased lipid peroxidation, as assessed by measuring malondialdehyde (MDA) content. Under the cold condition, transgenic plants also showed a significant increase in the activities of antioxidant enzymes (superoxide dismutase and catalase) and in relative water content (RWC) in comparison to non-transformed plants. Drought (polyethylene glycol in medium) and salinity (sodium chloride in medium) tolerance tests revealed that transgenic lines exhibited a higher tolerance for abiotic stresses than non-transformed plants. These findings indicate that the introduction of a bacterial mtlD gene into tomato conferred tolerance to abiotic stresses to the transformed tomato plants.

show abstract

Section: Introductionmentioning

confidence: 96%

Transgenic tomato cv. Pusa Uphar expressing a bacterial mannitol-1-phosphate dehydrogenase gene confers abiotic stress tolerance

Khare

Goyary

Singh

et al. 2010

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…

for stress responses (reviewed by Rajam et al 1998;Kumar et al 2006). It has been reported that the overexpression of PA biosynthetic genes like adc (Roy and Wu 2001;Capell et al 1998;2004) Abstract Polyamines (putrescine, spermidine and spermine) have been shown to be important in stress tolerance.

…”

mentioning

confidence: 99%

“…Plants have evolved various mechanisms to cope with stress conditions, and these include the shifts in the physiology of the plant as well as the expression of stress-associated genes leading to the formation of a wide variety of low molecular weight metabolites like mannitol, proline, glycine betaine and polyamines (Rajam et al 1998;Kumar et al 2006). Polyamines (PAs), putrescine (Put), spermidine (Spd) and spermine (Spm) play a pivotal role in plant response (or defense) to both abiotic and biotic stresses.…”

mentioning

confidence: 99%

Polyamine accumulation in transgenic eggplant enhances tolerance to multiple abiotic stresses and fungal resistance

Prabhavathi

Rajam

2007

Plant Biotechnology

111

View full text Add to dashboard Cite

for stress responses (reviewed by Rajam et al. 1998;Kumar et al. 2006). It has been reported that the overexpression of PA biosynthetic genes like adc (Roy and Wu 2001;Capell et al. 1998;2004) Abstract Polyamines (putrescine, spermidine and spermine) have been shown to be important in stress tolerance. Thus, the present study was undertaken with the aim to enhance stress tolerance in eggplant by introduction of a key polyamine biosynthetic gene arginine decarboxylase, adc under the control of a constitutive promoter of cauliflower mosaic virus, CaMV35S through Agrobacterium-mediated transformation. Several putative transgenic plants were generated and the transgene integration and expression was confirmed by PCR and Southern blot analyses, and RT-PCR analysis, respectively. These transgenic plants have shown an enhanced level of polyamines due to the increase in ADC enzyme activity. The diamine oxidase (an enzyme involved in putrescine and spermidine degradation) activity was also increased in these transgenic plants. Polyamine-accumulating transgenic plants exhibited an increased tolerance levels to multiple abiotic stresses such as salinity, drought, low and high temperature, and heavy-metal and resistance against fungal wilt disease caused by Fusarium oxysporium.Key words: Agrobacterium, arginine decarboxylase, eggplant, plant transformation, polyamines, stress tolerance.Plant Biotechnology 24, 273-282 (2007) Original PaperAbbreviations: ADC, arginine decarboxylase; DAO, diamine oxidase; MS, Murashige and Skoog; ODC, ornithine decarboxylase; PAs, polyamines; Put, putrescine; Spd, spermidine; Spm, spermine. This article can be found at http://www,jspcmb.jp/ Kasukabe et al. 2004; in plants like rice, tobacco, Arabidopsis and sweet potato, has resulted in increased tolerance to abiotic stresses. There is only one report on fungal resistance in transgenic tobacco plants overexpressing samdc gene (Waie and Rajam 2003).Therefore, the present work was aimed to generate transgenic eggplants with oat arginine decarboxylase (ADC, EC 4.1.1.19) gene, a key gene involved in PA biosynthesis, and evaluate such transgenic plants for PA levels and tolerance against both abiotic and biotic stresses under in vitro and in vivo growth conditions. Indeed, these transgenic eggplants have shown elevated PA content that could be correlated with increased tolerance to multiple abiotic stresses and fungal resistance. Materials and methods Plant material and plasmidThe seeds of eggplant (Solanum melongena L., cv. Pusa Purple Long) were obtained from the National Seeds Corporation, Indian Agricultural Research Institute, New Delhi. The Agrobacterium tumefaciens strain LBA4404 containing binary plasmid pMVR3ADC with oat adc gene under the control of cauliflower mosaic virus CaMV35S promoter and nopaline synthase terminator, and hygromycin phosphotransferase (hpt) as plant selection marker was used for eggplant transformation ( Figure 1A). Eggplant transformation and regenerationLeaf and cotyledon explants, collected from about thirty ...

show abstract

“…Koppenaal et al, 1991;McManus et al, 2000;Rajam et al, 1998;Sacher et al, 1985;Wingler, 2002). Previous studies quantifying soluble carbohydrates in Eucalyptus species in the field are rare, probably because the diversity of carbohydrates and polyols makes their comprehensive ("metabolomic") analysis difficult.…”

Section: Discussionmentioning

confidence: 99%

Variations saisonnières des hydrates de carbone, des cyclitols et des relations hydriques chez 3 espèces d’Eucalyptus de taxonomie contrastée, en plein champ et poussant sur un site commun

et al. 2010

View full text Add to dashboard Cite

Abstract• Alterations in plant chemistry underpin a suite of physiological adaptations to arid conditions. Qualitative and quantitative differences in leaf chemistry are found in the genus Eucalyptus correlating with physiological adaptation to aridity.• Here we investigate seasonal water relations of three field grown eucalypt species grown at a common site known to differ in their ability to accumulate the cyclic sugar alcohol, quercitol.• We show that quercitol contributes significantly to osmotic relations in field grown trees of Eucalyptus melliodora but is present only in trace amounts in E. rubida and E. obliqua.• Measured concentrations of quercitol account for the difference in osmotic potentials between species and can be interpreted as a mechanism for adaptation to low water availability. Mots-clés :Eucalyptus / potentiel hydrique / pression volume / ajustement osmotique Résumé -Variations saisonnières des hydrates de carbone, des cyclitols et des relations hydriques chez 3 espèces d'Eucalyptus de taxonomie contrastée, en plein champ et poussant sur un site commun.• Des altérations dans la chimie des plants soutiennent une série d'adaptations physiologiques à l'aridité. Des différences qualitatives et quantitatives dans la chimie des feuilles sont trouvées chez le genre Eucalyptus en corrélation avec les adaptations physiologiques à l'aridité.• Ici, nous étudions les relations hydriques saisonnières de 3 espèces d'Eucalyptus de plein champ, poussant sur un site commun, connues pour être différentes au plan de leur capacité à accumuler le quercitol.• Nous montrons que le quercitol contribue de manière significative aux relations osmotiques chez Eucalyptus melliodora mais est seulement présent à l'état de trace chez Eucalyptus rubida et Eucalyptus obliqua.• Les concentrations de quercitol qui ont été mesurées rendent compte des différences de potentiel osmotique observées entre les espèces et peuvent être interprétées comme un mécanisme d'adaptation à la faible disponibilité en eau.

show abstract

Genetic engineering of polyamine and carbohydrate metabolism for osmotic stress tolerance in higher plants

Cited by 31 publications

References 71 publications

Transgenic tomato cv. Pusa Uphar expressing a bacterial mannitol-1-phosphate dehydrogenase gene confers abiotic stress tolerance

Transgenic tomato cv. Pusa Uphar expressing a bacterial mannitol-1-phosphate dehydrogenase gene confers abiotic stress tolerance

Polyamine accumulation in transgenic eggplant enhances tolerance to multiple abiotic stresses and fungal resistance

Variations saisonnières des hydrates de carbone, des cyclitols et des relations hydriques chez 3 espèces d’Eucalyptus de taxonomie contrastée, en plein champ et poussant sur un site commun

Contact Info

Product

Resources

About