Genetic engineering of novel flower colour by suppression of anthocyanin modification genes in gentian

Nakatsuka, Takashi; Mishiba, Kei‐ichiro; Kubota, Akiko; Abe, Yoshiko; Yamamura, Saburo; Nakamura, Noriko; Tanaka, Yoshikazu; Nishihara, Masahiro

doi:10.1016/j.jplph.2009.08.007

Cited by 77 publications

(34 citation statements)

References 39 publications

(59 reference statements)

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“…Many researches are being performed to improve the flowers through gene silencing (Table 5). Seitz et al (2007) and Nakatsuka et al (2010) suggested that reduced accumulation of polyacylated anthocyanins by RNAi could cause modulations of flower colour. In 2005, Nishihara et al had applied RNAi-mediated silencing of chalcone isomerase (CHI) in tobacco and reported reduced pigmentation and change of flavonoid components in flower petals.…”

Section: Altered Colour/scent Of Flowersmentioning

confidence: 99%

RNA interference: concept to reality in crop improvement

Saurabh

Vidyarthi

Prasad

2014

Planta

194

100

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Section: Altered Colour/scent Of Flowersmentioning

confidence: 99%

RNA interference: concept to reality in crop improvement

Saurabh

Vidyarthi

Prasad

2014

Planta

194

100

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“…Therefore, we chose GtMYB3 as the first target gene for CRES-T. Previously, we found that the CaMV 35S promoter could not induce transgene expression in gentian because of consistent transcriptional gene silencing (Mishiba et al 2005(Mishiba et al , 2010. Instead of the CaMV 35S promoter, the Arabidopsis actin2 promoter was selected to control GtMYB3-SRDX (AtACT2pro; An et al 1996) for gentian transformation, because AtACT2pro successfully induces transgene expression in floral organs and leaves of gentian (data not shown).…”

Section: Production Of Gtmyb3-srdx-transgenic Gentian Plantsmentioning

confidence: 99%

“…Subsequently, RNAi suppression of anthocyanin biosynthetic genes, including CHS, flavonoid 3Ј,5Ј-hydroxylase (F3Ј5ЈH) and anthocyanidin synthase (ANS), was achieved and resulted in various flower colors, such as white, pink and pale blue, in transgenic gentian plants (Nakatsuka et al 2008c). More recently, suppression of F3Ј5ЈH and anthocyanin 5,3Ј-alomatic acyltransferase (5/3ЈAT), involved in the modification of anthocyanin gentiodelphin, resulted in novel lilac to pale blue colored flowers (Nakatsuka et al 2010). Thus, the silencing of flavonoid biosynthetic genes in gentian flowers was demonstrated to increase the diversity of flower colors by changing the composition and quantity of anthocyanin pigments in the petals (Nakatsuka et al 2008c(Nakatsuka et al , 2010Nishihara et al 2006).…”

mentioning

confidence: 99%

Production of picotee-type flowers in Japanese gentian by CRES-T

Nakatsuka¹,

Saito²,

Yamada³

et al. 2011

Plant Biotechnology

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Chimeric repressor gene-silencing technology (CRES-T) is an efficient gene suppression system in a wide variety of dicots and monocots. In this study, we demonstrated that the CRES-T system functions in Japanese gentian. A chimeric repressor of the anthocyanin biosynthetic regulator gene GtMYB3, under the control of the Arabidopsis actin2 promoter, was introduced into blue-flowered gentian. Of 12 transgenic lines, 2 exhibited a picotee flower phenotype with a lack of pigmentation in the lower part of the petal. HPLC analysis showed that the petals of these lines contained less anthocyanin and more flavone than the wild-type, suggesting competitive accumulation of these two types of compounds. The expressions of 'late' flavonoid biosynthetic genes, including F3H, F3Ј5ЈH, DFR and ANS, were strongly suppressed in petals of these transgenic plants. In contrast, the 'early' flavonoid biosynthetic genes, such as CHS and FNSII, were not affected. Since FNSII is expressed more strongly in the lower part of petals than in the upper part, the absence of pigmentation in the lower parts might be induced by flavone synthesis. These results demonstrated that the suppression of anthocyanin biosynthetic genes by CRES-T was successfully applied to Japanese gentian to change petal color; therefore, this system could be useful for generating novel flower colors and patterns. Transgenic plants produced in this study might be utilized as elite materials in the breeding of Japanese gentian in the near future.

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“…However, modern biotechnological techniques combined with in vitro cultures offer attractive alternative approaches to traditional cultivation techniques resulting both in the rapid micropropagation of valuable gentian species (Momčilović et al 1997;Hosokawa et al 1998), and providing an efficient source of secondary metabolites (Tiwari et al 2007;Cai et al 2009;Hayta et al 2011). Moreover, this technique enables modification of gentian genome for introduction of some desirable characteristics (Mishiba et al 2006;Nakatsuka et al 2010) and the conservation of gentian biodiversity (Tanaka et al 2004;Suzuki et al 2005;Mikuła et al 2008). An important prerequisite for the aforementioned use of in vitro cultures is the development of an efficient plant regeneration system from a suitable explant type that expresses a high degree of morphogenic potential.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the morphogenic potential of five Gentiana species in leaf mesophyll protoplast culture and ploidy stability of regenerated calli and plants

Tomiczak

Śliwińska

Rybczyński

2016

Plant Cell Tiss Organ Cult

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The morphogenic potential of five Gentiana species of medicinal and ornamental value, i.e.G. cruciata, G. kurroo, G. lutea, G. septemfida, and G. tibetica, was compared using agarose-bead leaf mesophyll protoplast culture. Modified Murashige and Skoog medium containing 2.0 mg l -1 1-naphthaleneacetic acid and 0.1 mg l -1 thidiazuron ensured the highest cell division frequency during both protoplast and protoplast-derived cell culture or callus formation. Indirect plant regeneration mainly via the somatic embryogenesis pathway was achieved for G. kurroo and G. tibetica with the greatest efficiency occurring with MS medium supplemented with 1.0 mg l -1 kinetin, 0.5 mg l -1 gibberellic acid, and 80 mg l -1 adenine sulfate. A considerable percentage of autopolyploid and aneuploid cells was detected in callus lines obtained from protoplasts using flow cytometry. The presence of polyploid cells in calli resulted in the regeneration of 85 % polyploid plants of G. kurroo and 14 % polyploids of G. tibetica. Chromosome counting and stomatal characteristic confirmed the ploidy variation among regenerants.

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Genetic engineering of novel flower colour by suppression of anthocyanin modification genes in gentian

Cited by 77 publications

References 39 publications

RNA interference: concept to reality in crop improvement

RNA interference: concept to reality in crop improvement

Production of picotee-type flowers in Japanese gentian by CRES-T

Comparison of the morphogenic potential of five Gentiana species in leaf mesophyll protoplast culture and ploidy stability of regenerated calli and plants

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