Genetic effect of Myf5 gene in rabbit meat quality traits

Abstract

The objective of this study was to investigate the effect of the polymorphism in the Myf5 gene on meat quality traits in the Ira and Tianfu Black rabbit breeds using polymerase chain reaction and DNA sequencing. A total of six SNPs and four haplotypes were found in Ira rabbits and only two SNPs were found in Tianfu Black rabbits. The two rabbit breeds had intermediate levels of genetic diversity according to their polymorphic information content values. The SNP association analysis in Ira indicated that SNP1-6 had a significant association with redness, yellowness and intramuscular fat values in the biceps femoris muscle, and also a significantly effect on redness in the longissimus dorsi muscle. The haplotype association analysis indicated that some haplotypes could be selected to get higher or lower meat redness, yellowness and intramuscular fat content in longissimus dorsi in Ira rabbits. Several SNPs and haplotypes of Myf5 identified here could be considered as molecular markers to improve the meat quality of Ira and Tianfu Black rabbits.

Expression patterns and genetic variation of the ovine skeletal muscle transcriptome of sheep from five Spanish meat breeds

“…those mapping to the MYF 5, FABP4 , PGAM2 and PRKAG3 genes might have functional consequences (Supplementary Table S5 ). The variability of the MYF5 gene has been associated with meat quality traits in pigs 34 , cattle 35 and rabbits 36 , and a missense substitution in the porcine PRKAG3 gene is a causal factor determining muscle glycolytic potential 37 . Allelic variation in the PGAM2 gene has been associated with drip loss percentage in pigs 38 and the polymorphism of FABP4 shows associations with meat quality traits in multiple species 39 .…”

On-Farm and Processing Factors Affecting Rabbit Carcass and Meat Quality Attributes

“…Accordingly, Wang et al (2017) proposed myogenic factor 5 (Myf5) single nucleotide polymorphism (SNPs) could be considered as a potential genetic marker for good meat quality selection in breeding programmes of Ira and Tianfu Black breed since it plays significant roles in muscle fiber formation and transcription of muscle-specific genes (Ujan et al, 2011). Apart from Myf5, calpastatin and myopalladin (MYPN) could also be considered as candidate genes for the genetic improvement of rabbit meat traits (Li et al, 2018;Wang et al, 2017). Further, Wang et al (2017) summarized specific gene sequences in Ira rabbits responsible for different meat qualities.…”

“…Apart from Myf5, calpastatin and myopalladin (MYPN) could also be considered as candidate genes for the genetic improvement of rabbit meat traits (Li et al, 2018;Wang et al, 2017). Further, Wang et al (2017) summarized specific gene sequences in Ira rabbits responsible for different meat qualities. Accordingly, genotypes of GG-AA-AA and AA-AG-AG could be used as genetic markers for increasing intramuscular fat and enhancing meat a*-and b*-values in BF muscles, respectively.…”

Novel Single Nucleotide Polymorphisms and Haplotype of MYF5 Gene Are Associated with Body Measurements and Ultrasound Traits in Grassland Short-Tailed Sheep

“…While in cattle, variation in MYF5 affects growth and meat quality traits including birth, fat, carcass weight, and body length [ 11 , 12 , 13 ]. In addition, variants of MYF5 have been reported to be associated with growth and reproductive traits in chicken [ 14 ], and showed differences in carcass and meat quality traits in pigeon [ 15 ] and rabbit [ 16 ].…”