Genetic diversity of wild and cultured Black Tiger Shrimp (Penaeus monodon) in the Philippines using microsatellites

Xu, Zhenkang; Primavera, Jurgenne H.; Peña, Leobert D. de la; Pettit, Priscilla; Belak, Jane; Alcivar–Warren, Acacia

doi:10.1016/s0044-8486(00)00535-4

Cited by 132 publications

(101 citation statements)

References 53 publications

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“…Microsatellite analyses have been used to assess genetic variation in several penaeid species, such as L. vannamei (Wolfus et al, 1997), Marsupenaeus japonicus (Moore et al, 1999), L. stylirostris (Bierne et al, 2000), Penaeus monodon (Tassanakajon et al, 1998;Brooker et al, 2000;Pongsomboon et al, 2000, Xu et al, 2001, Litopenaeus schmitti (Maggioni et al, 2003;Borrel et al, Genetics and Molecular Biology, 30, 4, 1156-1160(2007 Copyright by the Brazilian Society of Genetics. Printed in Brazil www.sbg.org.br 2004) and Litopenaeus setiferus (Ball and Chapman, 2003) but, however, little is known on the genetics of the Brazilian L. vannamei broodstocks Galetti Jr 2002, 2005;De Francisco and Galetti Jr, 2005;Gonçalves et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

2007

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The culture of the marine shrimp Litopenaeus vannamei has recently boosted the Brazilian shrimp industry. However, it is well known that selection methods based solely on phenotypic characteristics, a reduced number of breeders and the practice of inbreeding may promote a significant raise in the genetic similarity of the captive populations, leading to greater disease susceptibility and impairing both the growth and final size of the shrimps. We used four microsatellite loci to investigate genetic variation in three generations (F 5 , F 6 and F 7 ) of a closed and reared L. vannamei lineage. Although an accentuated heterozygosis deficit was detected, we also observed that the captive propagation of this lineage did not lead to a significant loss of genetic variability over the three generations studied. One possible reason for this is that the breeding conditions of this lineage were good enough to prevent any significant loss of genetic variability. However, three generations may have been insufficient to produce detectable changes in genetic frequencies in the loci studied. Alternatively, the microsatellite loci may have been non-neutral (biased) and related to the conditions in which the shrimps were kept, resulting in a similar allele pool in respect to these four microsatellites over the three generations studied. Any generalizations regarding microsatellite variation in closed shrimp lines may thus be incomplete and should be carefully analyzed.

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Section: Introductionmentioning

confidence: 99%

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

2007

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“…Population genetic data of Penaeus monodon based on several markers have been conducted, such as allozymes [4], mitochondrial DNA (mtDNA) fragment length polymorphism [5], randomly amplified polymorphic DNA (RAPD) [5,6], microsatellites [7,8,9], and sequence data [9,10,11]. However, it is essential to conduct wider genetic variation assessment using another molecular marker to provide holistic polymorphism database.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic variation in outbreed and inbreed giant tiger shrimp (Penaeus monodon Fabricius, 1798) using ISSR marker

Purnamaningrum¹,

Handayani²,

Trijoko³

et al. 2016

AIP Conference Proceedings

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Abstract. Giant tiger shrimp (Penaeus monodon Fabricius, 1798) is one of Indonesia's main aquaculture commodities. Four populations (G7, G8, G8iA and G8iJ) of tiger shrimp were obtained from broodstock center of Main Center for Brackishwater Aquaculture and Fisheries (MCBAF) Jepara, Central Java, Indonesia. G7 is the domesticated stock in MCBAF Jepara. Inbreed progeny was obtained from sibling mating of G7. Outbreed progeny was obtained from mating G7 with wild broodstock. Ten individuals of each population were evaluated for their genetic variation using ISSR marker. The following genetic variation parameters including Percentage of Polymorphic Bands for G7, G8, G8iA and G8iJ were 83.33%, 69.23%, 84.62%, and 72.00% respectively. Expected Heterozygosity values ranging from 0.235 (G8iJ) to 0.322 (G8iA). Shannon's Information Index ranged from 0.317 (G8) to 0.432 (G8iA). A number of Different Alleles was ranged from 1.536 (G8iJ) to 1.714 (G8iA). A number of Effective Alleles ranged from 1.405 (G8iJ) to 1.571 (G8iA). Outbreed population has higher genetic variation than inbreed population.

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“…As a result, molecular markers and associated tools, such as genetic maps, are currently available for some crustacean species, particularly for shrimps. Molecular markers have been applied, for example, for analyzing the genetic diversity of wild shrimp resources (Xu et al, 2001), for strain and species identification in penaeids (Calo-Mata et al, 2009), and for parentage analysis in the black tiger shrimp Penaeus monodon (Jerry et al, 2006). In addition, linkage maps have been generated for several penaeid shrimp species, in most cases by using amplified fragment length polymorphism (AFLP) (Li et al, 2003;Zhang et al, 2007;Staelens et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii

et al. 2011

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In this study, a female-specific DNA marker in the freshwater prawn Macrobrachium rosenbergii was identified through amplified fragment length polymorphism (AFLP). The AFLPderived sequence-characterized amplified region (SCAR) marker was tested in over 200 individuals, giving reproducible sex identification. Further molecular characterization of the sex-marker's genomic region (B3 kb long) revealed the presence of tandem and inverted repeats. The B3-kb sequence was identified both in male and female prawns, but with subtle differences: a deletion of 3 bp (present in female prawn but absent in male prawn) identified upstream of the SCAR marker sequence and two female-specific single-nucleotide polymorphisms, both indicating that male prawns are homozygous, whereas female prawns are heterozygous in this locus. Fluorescent in situ hybridization showed the B3-kb sequence to be unique: to the best of our knowledge, this is the first report of a unique sex-specific sequence observed in situ in crustaceans. The sex-specific marker identified in M. rosenbergii may have considerable applied merit for crustacean culture in that it will enable the determination of genetic sex at early developmental stages when phenotypic differences are not identifiable.

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Genetic diversity of wild and cultured Black Tiger Shrimp (Penaeus monodon) in the Philippines using microsatellites

Cited by 132 publications

References 53 publications

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

Assessment of genetic variation in outbreed and inbreed giant tiger shrimp (Penaeus monodon Fabricius, 1798) using ISSR marker

Isolation and characterization of a female-specific DNA marker in the giant freshwater prawn Macrobrachium rosenbergii

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