Genetic diversity of Ralstonia solanacearum, A phytopathogenic bacterium infecting horticultural plants in Java, Indonesia

Hemelda, Niarsi Merry; Safitri, Ramadhani; Suhandono, Sony

doi:10.13057/biodiv/d200209

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…All isolates of R. pseudosolanacearum used in this study had the same phylotype, apart from differences in plant host (tomato, eggplant, and tobacco) and region of origin (Central Java and West Java). The results are consistent with the study of R. solanacearum genetic diversity in horticultural crops on Java island (Hemelda et al 2019). A total of 20 out of 21 R. solanacearum isolates from Java Island were phylotype I.…”

Section: Phylotype and Sequevar Of Ralstonia Pseudosolanacearumsupporting

confidence: 90%

Physio-biochemical, molecular characterization, and phage susceptibility of Ralstonia pseudosolanacearum associated with tomato (Solanum lycopersicum) and eggplant (Solanum melongena)

2022

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Abstract. Nurdika AAH, Arwiyanto T, Sulandari S. 2022. Physio-biochemical, molecular characterization, and phage susceptibility of Ralstonia pseudosolanacearum associated with tomato (Solanum lycopersicum) and eggplant (Solanum melongena). Biodiversitas 23: 5149-5158. Ralstonia solanacearum is a plant pathogen that causes bacterial wilt diseases in various plant species. The high diversity of these bacteria strains is the basis for their grouping into the R. solanacearum species complex (RSSC). RSSC associated with tomato and eggplant in Indonesia are commonly known as R. solanacearum. It is necessary to characterize R. solanacearum which infects tomatoes and eggplant according to the latest classification. The high diversity of strains also affects their susceptibility to bacteriophages as host-specific biocontrol agents. This research was conducted by characterizing R. solanacearum isolates from different locations based on their physio-biochemical properties, biovar, virulence, phylotype, sequevar, and susceptibility to 12 bacteriophage isolates. The phylotype identification was carried out using multiplex polymerase chain reaction with several specific primers for R. solanacearum. Amplification and sequencing based on the egl gene region were carried out to determine the sequevar of R. solanacearum isolates. As a result, isolates RS18, RS19, RS23, and RS24 had morphological, physio-biochemical, and biovar characteristics according to Ralstonia pseudosolanacearum phylotype I, biovar 3. The two most virulent isolates, RS19 and RS24 were identified as sequevar 14. RS19 and RS24 isolates were susceptible to 7 of the 12 bacteriophage isolates used in this study. Variations of bacteriophage isolates resulted in different plaque morphology which could be attributed to R. pseudosolanacearum susceptibility and bacteriophage virulence.

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Section: Phylotype and Sequevar Of Ralstonia Pseudosolanacearumsupporting

confidence: 90%

Physio-biochemical, molecular characterization, and phage susceptibility of Ralstonia pseudosolanacearum associated with tomato (Solanum lycopersicum) and eggplant (Solanum melongena)

2022

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“…Bacterial wilt disease caused by Ralstonia solanacearum is a devastating soil-borne vascular disease that severely affects tomato (Solanum lycopersicum L.), leading to a 100% yield loss (Genova et al 2013;Hemelda et al 2019). R. solanacearum infected tomato plants by colonizing the plant rhizosphere, where the pathogen must compete with the other bacterial taxa (Hibbing et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

et al. 2020

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Abstract. Navitasari L, Joko T, Murti RH, Arwiyanto T. 2020. Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum. Biodiversitas 21: 4888-4895. Bacterial wilt disease caused by Ralstonia solanacearum is a devastating soil-borne vascular disease of tomato leading to a 100% yield loss. One of the alternatives to suppress the infestation of R. solanacearum infestation is the application of grafting techniques, which has been studied and successfully practiced by tomato growers. However, the infestation mode of R. solanacearum and the rhizobacterial community structure in grafted tomato plants are poorly reported. In this study, the rhizobacterial community structure in grafted tomato plants infected by R. solanacearum was investigated. The experiment was conducted on tomato germplasms with the implementation of tube grafting using resistant rootstocks (Amelia from Indonesia, H.7996 from Asian Vegetable Research Development Center/AVRDC) and susceptible scion (Servo from Indonesia). The rhizobacterial community structure was analyzed by metagenomic study under 16S rRNA genes sequencing with a distinct region (16SV3-V4) that was amplified using a specific primer (16SV4: 515F-806R) 5’-GTGCCAGCMGCCGCGGTAA and 5’GGACTACHVHHHTWTCTAAT. The results indicated that the grafted tomato plants and resistant rootstocks that were infected by R. solanacearum showed significantly lower intensity of bacterial wilt disease compared to the susceptible scion. The rhizobacterial community structure in the grafted tomato plants infected by R. solanacearum was indicated by predominant phyla of Proteobacteria, Firmicutes, and Actinobacteria with dominant genera of Pseudomonas and Bacillus. Besides, significant difference was also indicated by species of Geitlerinema sp. in the grafted tomatoes infected by R. solanacearum.

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QTL identification, fine mapping, and marker development for breeding peanut (Arachis hypogaea L.) resistant to bacterial wilt

Sun

Liu

et al. 2022

Theor Appl Genet

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Key message A major QTL, qBWA12, was fine mapped to a 216.68 kb physical region, and A12.4097252 was identified as a useful KASP marker for breeding peanut varieties resistant to bacterial wilt. Abstract Bacterial wilt, caused by Ralstonia solanacearum, is a major disease detrimental to peanut production in China. Breeding disease-resistant peanut varieties is the most economical and effective way to prevent the disease and yield loss. Fine mapping the QTLs for bacterial wilt resistance is critical for the marker-assisted breeding of disease-resistant varieties. A recombinant inbred population comprising 521 lines was used to construct a high-density genetic linkage map and to identify QTLs for bacterial wilt resistance following restriction-site-associated DNA sequencing. The genetic map, which included 5120 SNP markers, covered a length of 3179 cM with an average marker distance of 0.6 cM. Four QTLs for bacterial wilt resistance were mapped on four chromosomes. One major QTL, qBWA12, with LOD score of 32.8–66.0 and PVE of 31.2–44.8%, was stably detected in all four development stages investigated over the 3 trial years. Additionally, qBWA12 spanned a 2.7 cM region, corresponding to approximately 0.4 Mb and was fine mapped to a 216.7 kb region by applying KASP markers that were polymorphic between the two parents based on whole-genome resequencing data. In a large collection of breeding and germplasm lines, it was proved that KASP marker A12.4097252 can be applied for the marker-assisted breeding to develop peanut varieties resistant to bacterial wilt. Of the 19 candidate genes in the region covered by qBWA12, nine NBS-LRR genes should be further investigated regarding their potential contribution to the resistance of peanut against bacterial wilt.

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Genetic diversity of Ralstonia solanacearum, A phytopathogenic bacterium infecting horticultural plants in Java, Indonesia

Cited by 3 publications

References 33 publications

Physio-biochemical, molecular characterization, and phage susceptibility of Ralstonia pseudosolanacearum associated with tomato (Solanum lycopersicum) and eggplant (Solanum melongena)

Physio-biochemical, molecular characterization, and phage susceptibility of Ralstonia pseudosolanacearum associated with tomato (Solanum lycopersicum) and eggplant (Solanum melongena)

Rhizobacterial community structure in grafted tomato plants infected by Ralstonia solanacearum

QTL identification, fine mapping, and marker development for breeding peanut (Arachis hypogaea L.) resistant to bacterial wilt

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