Genetic diversity of porcine enteric caliciviruses in pigs raised in Rio de Janeiro State, Brazil

Cunha, Juliana Bragazzi; Mendonça, Marcos César Lima de; Miagostovich, Marize Pereira; Leite, José Paulo Gagliardi

doi:10.1007/s00705-010-0695-z

Cited by 25 publications

(28 citation statements)

References 29 publications

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“…Even though different ages of pigs were tested, NoVs were detected only in finisher pigs (7). A Canadian study reported 25% prevalence (41), and many other studies found lower prevalence rates: 2% to 4.6% in Europe (5, 42), 9% in New Zealand (43), 8% in Brazil (44), and Ͻ1% to 15% in Asia (8,15,22). These differences could be due to different geographical locations, different ages of pigs sampled, or the use of different detection methods and primers with various specificities.…”

Section: Discussionmentioning

confidence: 96%

Prevalence of Porcine Noroviruses, Molecular Characterization of Emerging Porcine Sapoviruses from Finisher Swine in the United States, and Unified Classification Scheme for Sapoviruses

et al. 2013

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Noroviruses (NoVs) and sapoviruses (SaVs) are important human pathogens. Although the involvement of porcine NoVs in disease in pigs is unclear, they are genetically and antigenically closely related to human NoVs. Human NoV-like strains have been detected in pigs, raising public health concerns of potential interspecies transmission. Porcine SaVs are highly diverse and emerging in swine populations. Recently, at least three new genogroups of porcine SaVs have been proposed. In this study, we tested 413 pooled fecal samples collected from apparently healthy finisher pigs in North Carolina swine farms during 2009. Reverse transcription (RT)-PCR coupled hybridization assays were performed to detect known porcine NoVs. The overall prevalence of porcine NoVs determined was 18.9% based on this method. Samples were then tested by RT-PCR targeting the 5= end of the capsid region for genogroup II (GII) NoVs, a group which includes human NoVs, followed by sequence analysis. All NoVs identified belonged to typical porcine NoV genotypes, and no human NoV-like strains were detected in specimens from these pigs. Porcine NoV-negative samples (n ‫؍‬ 335) were subsequently screened using universal calicivirus primers, and 17 SaV strains were confirmed by sequencing. Based on the partial RNA-dependent RNA polymerase (RdRp) region, they clustered with GIII, GVII, and GVIII and with currently unclassified SaVs. According to analysis of the complete capsid sequences, 7 representative strains clustered with GVII, GVIII, and GIX? SaVs. We tentatively classified SaVs into 14 genogroups based on the complete capsid protein VP1. In summary, porcine NoVs and highly divergent SaVs were present in North Carolina finisher pigs.

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Section: Discussionmentioning

confidence: 96%

Prevalence of Porcine Noroviruses, Molecular Characterization of Emerging Porcine Sapoviruses from Finisher Swine in the United States, and Unified Classification Scheme for Sapoviruses

et al. 2013

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“…Molecular diagnostics performed in different countries have established a worldwide distribution of NoV in pigproduction systems (Wolf et al 2009;Keum et al 2009;L'Homme et al 2009;Mijovski et al 2010;Cunha et al 2010a). In general, the reported rates of PoNoV detection were low, ranging from 0 to 2 % (Martínez et al 2006;Keum et al 2009;Mijovski et al 2010) to 20 % (Wang et al 2006b).…”

Section: Resultsmentioning

confidence: 93%

“…Cunha et al (2010a) reported 8 % (20/261) of PoNoV detection rate in finisher pigs in Rio de Janeiro state, Brazil. A possible explanation for this significant difference between the frequencies of NoV in pigs in the same country is the RT-PCR sensitivity, which can be higher when using specific primers for PoNoV detection.…”

Section: Resultsmentioning

confidence: 96%

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High frequency of porcine norovirus infection in finisher units of Brazilian pig-production systems

Silva

Alfieri

Barry

et al. 2014

Trop Anim Health Prod

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Norovirus (NoV) is a member of the Caliciviridae family and is considered an emerging human enteric pathogen. NoVs are detected in farm animals such as cattle, sheep and pigs. Porcine NoV (PoNoV) is widespread worldwide, but frequency of infection is often low. This study aimed to investigate the natural PoNoV infection from adult animals of an important Brazilian pig-production region. Faecal samples (n = 112) of asymptomatic pigs aged 9 to 24 weeks old were collected from 16 grower-to-finish herds located in Paraná state, Brazilian Southern region, and evaluated for PoNoV presence. A reverse transcription-polymerase chain reaction (RT-PCR) assay was performed using specific primers that target a conserved region of the virus capsid gene (VP1). PoNoV was detected in 58 (51.8%) of the 112 faecal samples and in 14 (87.5%) of the 16 herds evaluated. Six of the obtained amplicons were submitted to phylogenetic genotyping analysis. The higher nucleotide (86.5-97.4%) and amino acid (100%) similarities of the sequences in this study were with the representative strains of the porcine NoV genogroup II genotype 11 (PoNoV GII-11). These results reveal that PoNoV infection is endemic in one of the most important pork production areas of Brazil and that the PoNoV GII-11 is prevalent in this region.

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“…Emerging viral infections of pigs have been recently reported in Brazil; these include infections due to rotaviruses B and C (Médici et al 2011), caliciviruses (Barry et al 2008, Cunha et al 2010, kobuvirus , and hepatitis E virus (Gardinali et al 2012). The associations of these emerging viruses and of TTSuV with disease in pigs have not been elucidated (Meng 2012).…”

Section: Discussionmentioning

confidence: 99%

Torque teno sus virus (TTSuV) infection at different stages of pig production cycle

Leme

Alfieri

2013

Pesq. Vet. Bras.

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Pesq. Vet. Bras. 33 (7) It has been demonstrated that TTSuV might increase the severity of other important viral diseases with economic and public health impacts. At present, there is no information on the age distribution of pigs infected with TTSuV in Brazilian herds. This study evaluated the frequency of TTSuV infection in pigs at different stages of production. Fecal samples (n=190) from pigs at 1 to 24 weeks of age and from breeders at 6 farrow-to-weaning (up to 8 weeks of age) and 9 grower-to-finish (9 weeks of age onwards) farms in the western region of Paraná state, Brazil, were evaluated by PCR. Fragments of the 5' UTRs of TTSuV1 and/or TTSuVk2 DNAs were identified in 126 (66.3%) of the fecal samples. Significant differences were found with the percentages of positive samples for TTSuV1, TTSuVk2, and mixed infections by both genera between and within the different pig production stages. Fecal samples from the grower-to-finish farms had TTSuV detection rates (90.1%; 64/71) that were significantly (p<0.05) higher than those from the farrow-to-weaning farms (52.1%; 62/119). TTSuV detection was significantly (p<0.05) more frequent in finisher pigs than in the animals from the other stages. The UTR nucleotide sequences in this study presented higher similarities to strains from Norway (96%, TTSuV1), and Argentina and China (97.1%, TTSuVk2). These results suggest that TTSuV infection has spread to pigs of all production stages and that the viral infection rate increases with the age of the animals. In the western region of Paraná state, Brazil, TTSuV1 and TTSuVk2-induced infections were more frequently observed in suckling piglets and finisher pigs, respectively. Phylogenetic analysis pointed out the possibility of different strains of TTSuV1 and TTSuVk2 circulating in pig herds of Brazil. há informações sobre a distribuição da infecção pelo TTSuV, de acordo com a faixa etária, em rebanhos suinícolas brasileiros. Este estudo avaliou a frequência da infecção pelo TTSuV nas diferentes categorias de produção de suí-nos. Amostras fecais (n=190) de suínos com 1 a 24 semanas de idade e de reprodutores provenientes 6 unidades produtoras de leitão (até 8 semanas de idade) e 9 unidades de terminação (9 semanas de idade em diante) da região oeste do Paraná, Brasil, foram avaliadas pela técnica de PCR. Fragmentos da região 5' UTR do DNA do TTSuV1 e/ ou TTSuVk2 foram identificados em 126 (66,3%) amostras fecais. Diferenças significativas foram encontradas em relação às porcentagens de amostras positivas para o TTSuV1, Pesq. Vet. Bras. 33 (7)

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Genetic diversity of porcine enteric caliciviruses in pigs raised in Rio de Janeiro State, Brazil

Cited by 25 publications

References 29 publications

Prevalence of Porcine Noroviruses, Molecular Characterization of Emerging Porcine Sapoviruses from Finisher Swine in the United States, and Unified Classification Scheme for Sapoviruses

Prevalence of Porcine Noroviruses, Molecular Characterization of Emerging Porcine Sapoviruses from Finisher Swine in the United States, and Unified Classification Scheme for Sapoviruses

High frequency of porcine norovirus infection in finisher units of Brazilian pig-production systems

Torque teno sus virus (TTSuV) infection at different stages of pig production cycle

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