2018
DOI: 10.1645/18-9
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Abstract: The genotyping of 25 isolates of Toxoplasma gondii from free-range chickens in the state of Bahia, Brazil, was performed by PCR-restriction fragment length polymorphism using 11 genetic markers: SAG1, 5'+3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3. The analysis revealed 8 genotypes, 3 of which had not been previously reported. Four genotypes were represented by single isolates, whereas the other genotypes were represented by 2 or more isolates. Five isolates showed mixed infecti… Show more

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Cited by 13 publications
(10 citation statements)
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“…Bioassay results suggest a low degree of virulence for isolates obtained here, since none of the mice infected during the isolation process presented acute symptoms or died of toxoplasmosis. Our CS3 typing results disagree with those of previous studies carried out with Brazilian and Chinese isolates of different host origins that report high mortality rates (normally above 80%) associated with type I or II alleles for the CS3 gene and low (3.7–9.3%) or null rates with type III alleles [ 34 , 57 60 ]. Contradictory results were already exposed within avirulent Brazilian isolates presenting type I [ 61 ] or type II [ 62 ] alleles for the CS3 locus.…”
Section: Discussioncontrasting
confidence: 99%
“…Bioassay results suggest a low degree of virulence for isolates obtained here, since none of the mice infected during the isolation process presented acute symptoms or died of toxoplasmosis. Our CS3 typing results disagree with those of previous studies carried out with Brazilian and Chinese isolates of different host origins that report high mortality rates (normally above 80%) associated with type I or II alleles for the CS3 gene and low (3.7–9.3%) or null rates with type III alleles [ 34 , 57 60 ]. Contradictory results were already exposed within avirulent Brazilian isolates presenting type I [ 61 ] or type II [ 62 ] alleles for the CS3 locus.…”
Section: Discussioncontrasting
confidence: 99%
“…The CS3 locus has been described previously as a highly predictive marker of mortality in mice inoculated with T. gondii isolates (16). Our CS3 typing results disagree with previous Brazilian and Chinese studies reporting high mortality rates associated with the type I or II alleles of the CS3 gene and low or null rates associated with the type III alleles (16,39,40). However, opposite results have been reported in studies from Brazil (41,42) and Spain (14).…”
Section: Discussioncontrasting
confidence: 99%
“…gondii virulence as analyzing it is a potentially faster and more practical alternative to performing the biological assay in mice [ 13 , 24 – 26 ]. The results of the cluster analysis corroborated the findings of other studies in Brazil that demonstrated that there were few atypical or recombinant isolates associated with genotype II [ 12 15 , 21 , 22 , 25 ].…”
Section: Resultssupporting
confidence: 86%