Genetic diversity of Hungarian indigenous chicken breeds based on microsatellite markers

Abstract

Six local chicken breeds are registered in Hungary and are regarded as Hungarian national treasures: Hungarian White, Yellow and Speckled, and Transylvanian Naked Neck White, Black and Speckled. Three Hungarian academic institutes have maintained these genetic resources for more than 30 years. The Hungarian Yellow, the Hungarian Speckled and the Transylvanian Naked Neck Speckled breeds were kept as duplicates in two separate subpopulations since time of formation of conservation flocks at different institutes. In this study, we investigated genetic diversity of these nine Hungarian chicken populations using 29 microsatellite markers. We assessed degree of polymorphism and relationships within and between Hungarian breeds on the basis of molecular markers, and compared the Hungarian chicken populations with commercial lines and European local breeds. In total, 168 alleles were observed in the nine Hungarian populations. The F(ST) estimate indicated that about 22% of the total variation originated from variation between the Hungarian breeds. Clustering using structure software showed clear separation between the Hungarian populations. The most frequent solutions were found at K = 5 and K = 6, respectively, classifying the Transylvanian Naked Neck breeds as a separate group of populations. To identify genetic resources unique to Hungary, marker estimated kinships were estimated and a safe set analysis was performed. We show that the contribution of all Hungarian breeds together to the total diversity of a given set of populations was lower when added to the commercial lines than when added to the European set of breeds.

Assessing the genetic diversity of five Tanzanian chicken ecotypes using molecular tools

“…From molecular genetic marker analyses, the expected and observed heterozygosity estimates were higher in Tanzanian indigenous chickens compared with commercial breeds reported earlier (Granevitze et al, 2007;Muchadeyi et al, 2007;Bodzar et al, 2009;Fosta et al, 2011). Furthermore, the differentiation between Tanzanian chicken ecotypes (F ST 0.048) was found to be smaller than between commercial chicken lines.…”

Evaluation of the genetic diversity and population structure of five indigenous and one introduced Chinese goose breeds using microsatellite markers

“…Due to this reason, the clustering algorithm implemented in STRUCTURE (Pritchard et al 2000) was designed to overcome the limitations inherent in phylogenetic tree models and has been applied to infer the genetic structure in several species (Á lvarez et al 2004;Bodzsar et al 2009;Collins et al 2011). STRUCTURE analysis defined the degree of admixture for each individual animal.…”

Preliminary Studies on Genetic Diversity of Selected Polish Local Chicken Varieties

“…The genetic variability of Polish local chicken varieties, reflected by the expected heterozygosity index, was comparable with European local chickens. The H E values of indigenous chickens from France, Hungary and Italy ranged from 0.43 to 0.63 (Berthouly, et al, 2008), from 0.44 to 0.56 (Bodzsár, et al, 2009) and from 0.243 to 0.559 (Zanetti, et al, 2010), respectively. Our results of genetic diversity estimations of Greenleg Partridge were approximately equivalent to the results of the Aviandiv project (H O = 0.362; H E = 0.442; F IS = 0.175; Hillel, et al, 2003).…”