Genetic diversity of French common wheat germplasm based on gliadin alleles

Metakovsky, E. V.; Branlard, Gérard

doi:10.1007/s001220050729

Cited by 85 publications

(56 citation statements)

References 25 publications

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“…The data on the high frequency of the allele Gli B1l (the 1BL/1RS translocation) in the Ukrainian Central Forest Steppe cultivars are in good agreement with the data obtained for French cultivars. Metakovsky and Branlard [12] revealed the high frequency of the allele Gli B1l among cultivars grown in the North of France, which are more resistant to cold.…”

Section: Variation At Storage Protein Loci In Winter Common Wheat Culmentioning

confidence: 99%

“…Electrophoresis of HMW GS was car ried out by the procedure of Laemmli [18] in 10 % resolving gel. Gliadin alleles were identified using the catalogue of Metakovsky [3] supplemented by alleles presented in further studies [12,14,19]. The Gli B5b allele was identified from the charac teristic pattern (two ω components), as presented in [14].…”

mentioning

confidence: 99%

“…The Gli B5b allele was identified from the charac teristic pattern (two ω components), as presented in [14]. Cultivars lacking these components carry the Gli B5a allele (null allele) [12]. Alleles of HMW GS were identified by the catalogue of Payne and Lawrence [6].…”

mentioning

confidence: 99%

“…Four gliadin alleles identified in the Ukrainian Forest Steppe cultivars (Gli A1w, Gli A1x, Gli A1y and Gli B1x) are absent in the basic catalogue of Metakovsky [3] and among alleles presented in further studies [12,14,19], as well as in the last version of the Catalogue of Gene Symbols [4]. Electrophoretic patterns of these alleles are given in Figure. The allele Gli A1w (a specific secalin block) is a marker of the wheat rye 1AL/1RS translocation that first appeared in the cultivar Amigo.…”

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confidence: 99%

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Variation at storage protein loci in winter common wheat cultivars of the central forest-steppe of Ukraine

et al. 2009

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Section: Variation At Storage Protein Loci In Winter Common Wheat Culmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Variation at storage protein loci in winter common wheat cultivars of the central forest-steppe of Ukraine

et al. 2009

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“…In addition, the variability found in Indian wheats (Triticum aestivum L.), H = 0.875 (Sewa et al 2005), and in durum wheat landraces in the northwest of Iran and Azerbaijan, H = 0.900 and H = 0.854, respectively (Zaefizadeh et al 2010) was lower than that revealed in the Algerian collection. This variation was also much higher than that of Spanish spelt wheat, H = 0.703 (Caballero et al 2004), Spanish common wheat, H = 0.844 (Metakovsky et al 2000), H = 0.804 (Ruiz et al 2002) and French common wheat, H = 0.714 (Metakovsky & Branlard 1998).…”

Section: Resultsmentioning

confidence: 69%

The genetic diversity of gliadins in Aegilops geniculata from Algeria

Medouri¹,

Bellil²,

Khelifi³

2015

CZECH J GENET PLANT

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Medouri A., Bellil I., Khelifi D. (2015): The genetic diversity of gliadins in Aegilops geniculata from Algeria. Czech J. Genet. Plant Breed., 51: 9-15.The gliadins of the wild wheat Aegilops geniculata represent a valuable gene pool in breeding for bread making quality. The genetic diversity of gliadins in A. geniculata was studied among 36 of its accessions, collected in the north of Algeria, using acid polyacrylamide-gel electrophoresis (Acid-PAGE). In total, sixty-one polymorphic bands and 35 gliadin patterns were identified. Twenty-eight different bands and 34 patterns were found in the ω-gliadin region, 13 polymorphic bands and 33 patterns for γ-gliadins, 12 bands and 34 different patterns for β-gliadins and eight bands in combination resulted in 25 different patterns in the α-gliadin zone. Thirty-five patterns were found for each of the Gli-1 (γ/ω region) and Gli-2 (α/β region) loci. The genetic diversity index (H) was higher for ω-gliadins (0.968), followed by γ-and β-gliadins (0.964 and 0.961, respectively), and the lowest value was detected in α-gliadin patterns (0.944). Cluster analysis based on Ward's method divided the analysed collection into five separated groups in which genetic diversity did not follow the geographical distribution. The polymorphism observed in the electrophoretic patterns highlights close correlations between bioclimatic features and some ω-gliadin proteins.

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Composition of proteins in wheat grain streams obtained by sieve classification

et al. 2007

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Sieve classification was used to prepare grain streams of distinct protein content and composition. Both reverse-phase high-performance liquid chromatography and two-dimensional electrophoresis revealed differences in protein composition between large (F1), medium (F2-F3), and small (F4) kernel fractions of three Polish cultivars: Sukces and Tonacja (winter wheats) and Nawra (spring wheat). Statistical analysis indicated that grain protein composition is affected by the cultivar and kernel size, as well as by interaction of these traits. The albumin/globulin and glutenin fractions showed a tendency to decrease with diminishing kernel size. The gliadin content was affected to a greater extent by the kernel size than by the wheat genotype. The highest content of gliadins was observed in the smallest and the largest kernel fractions.

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Genetic diversity of French common wheat germplasm based on gliadin alleles

Cited by 85 publications

References 25 publications

Variation at storage protein loci in winter common wheat cultivars of the central forest-steppe of Ukraine

Variation at storage protein loci in winter common wheat cultivars of the central forest-steppe of Ukraine

The genetic diversity of gliadins in Aegilops geniculata from Algeria

Composition of proteins in wheat grain streams obtained by sieve classification

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