2014
DOI: 10.1590/s1984-29612014055
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Abstract: The aim of this study was to characterize Ehrlichia canis strains from naturally infected dogs in Rio de Janeiro, Brazil. In addition, all the clinical and hematological findings observed in these dogs were reported. PCR targeting the 16S rRNA gene was used for diagnostic purposes, and the TRP19 and TRP36 genes were sequenced to evaluate the genetic diversity. Fifteen samples were positive for E. canis. The polymerase chain reaction for the TRP19 gene resulted in 11 amplicons (11/15), which were cloned into th… Show more

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Cited by 13 publications
(18 citation statements)
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“…The transconjugants were selected in LB agar containing XGal (50 μg/mL) and ampicillin (100 μg/mL) after incubation overnight at 37 °C. White colonies were chosen and grown in LB broth with 100 μg/mL ampicillin under agitation (250 rpm) at 37 °C, as previously described [23]. The plasmid vector containing the insert was extracted using the alkaline lysis extraction method [24] and was sequenced with primer pairs targeting the htrA gene to confirm the cloned DNA fragment.…”
Section: Methodsmentioning
confidence: 99%
“…The transconjugants were selected in LB agar containing XGal (50 μg/mL) and ampicillin (100 μg/mL) after incubation overnight at 37 °C. White colonies were chosen and grown in LB broth with 100 μg/mL ampicillin under agitation (250 rpm) at 37 °C, as previously described [23]. The plasmid vector containing the insert was extracted using the alkaline lysis extraction method [24] and was sequenced with primer pairs targeting the htrA gene to confirm the cloned DNA fragment.…”
Section: Methodsmentioning
confidence: 99%
“…The 100% identity between the Brazilian and North American sequences of E. canis demonstrates the high degree of conservation for the gp19 gene. Ferreira [4] found similar results Table 3 Amino acid differences in the variable region and number of repeats in the TRP region of the partial sequence of the polypeptide deduced from TRP36 among Ehrlichia canis sequences and obtained from dogs from the mesoregions of the state of Rio de Janeiro Sequences Variable region a TRP* 10 19 23 31 46 70 72 78 79 86 111 145-240 Ehrlichia canis-Jake, when analyzing samples from the Metropolitan region of Rio de Janeiro. Chen [13] found that the E. canis gp19 gene is highly conserved by analyzing 153 samples from dogs with clinical signs at a Veterinary Hospital in Taiwan/China with PCR.…”
Section: Discussionmentioning
confidence: 56%
“…Ehrlichia canis presents a ubiquitous distribution, and this distribution is associated with the availability of the vector tick Rhipicephalus sanguineus sensu lato (s.l). In the state of Rio de Janeiro, the prevalence ranges from 5 to 16% [3,4]. In the state of São Paulo, the prevalence is 27.6% [5]; the frequency of dogs infected with E. canis can be as high as 65% [6].…”
Section: Introductionmentioning
confidence: 99%
“…Subsequently, new isolates were obtained from different Brazilian regions, and their genetic diversity was examined based on amino acid sequences, which were deduced from the nucleotide sequence of the trp36 gene (Doyle et al, 2005;Aguiar et al, 2013). Ferreira et al (2014) also performed a phylogenetic study based on the trp36 gene sequences of E. canis strains infecting dogs in Rio de Janeiro.…”
Section: /12mentioning
confidence: 99%