Genetic diversity of Ehrlichia canis in Brazil

Aguiar, Daniel Moura de; Zhang, X.; Melo, Andréia Lima Tomé; Pacheco, Thabata Anjos; Meneses, André Marcelo Conceição; Zanutto, Marcelo de Souza; Horta, Maurício Cláudio; Santarém, Vamilton Álvares; Camargo, Luís Marcelo Aranha; McBride, Jere W.; Labruna, Marcelo B.

doi:10.1016/j.vetmic.2013.02.015

Cited by 54 publications

(70 citation statements)

References 13 publications

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“…Since the sequence 56C was grouped with the sequence Cuiabá 16 and the identicalness between the sequences of the N-terminal region was high (100%), these sequences formed a new branch in the US genogroup, thus confirming that in the state of Rio de Janeiro, the Brazilian genogroup may also be circulating, since the Cuiabá 16 nucleotide sequence and the 56C nucleotide sequence showed 100% identicalness with the N-terminal regions of sequences from the Brazilian genogroup (Cuiabá 1, Belém and Londrina). On the other hand, the animal infected with this strain (56C) did not have any history of travel to any of the regions where the Brazilian genogroup had already been described, which reinforces the idea suggested by Aguiar et al (2013) that recombination between strains of E. canis exists in Brazil, where the prevalence is high.…”

Section: Discussionsupporting

confidence: 83%

“…The phylogenetic analysis among the Brazilian and other worldwide E. canis strains was performed based on the model proposed by Aguiar et al (2013).…”

Section: Resultsmentioning

confidence: 99%

“…In the central-western region, a hybrid (Cuiabá 16) has been described, possibly created through an association between two strains of different genogroups. The Brazilian genogroup has not been described in the southeastern region (AGUIAR et al, 2013). Since the sequence 56C was grouped with the sequence Cuiabá 16 and the identicalness between the sequences of the N-terminal region was high (100%), these sequences formed a new branch in the US genogroup, thus confirming that in the state of Rio de Janeiro, the Brazilian genogroup may also be circulating, since the Cuiabá 16 nucleotide sequence and the 56C nucleotide sequence showed 100% identicalness with the N-terminal regions of sequences from the Brazilian genogroup (Cuiabá 1, Belém and Londrina).…”

Section: Discussionmentioning

confidence: 97%

“…In Brazil, the gene encoding the protein TRP36 has been used for bacterial typing, and new genogroups have emerged along with evidence of genetic recombination between different strains of E. canis (AGUIAR et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil

Ferreira

Cerqueira

Castro

et al. 2014

Rev. Bras. Parasitol. Vet.

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The aim of this study was to characterize Ehrlichia canis strains from naturally infected dogs in Rio de Janeiro, Brazil. In addition, all the clinical and hematological findings observed in these dogs were reported. PCR targeting the 16S rRNA gene was used for diagnostic purposes, and the TRP19 and TRP36 genes were sequenced to evaluate the genetic diversity. Fifteen samples were positive for E. canis. The polymerase chain reaction for the TRP19 gene resulted in 11 amplicons (11/15), which were cloned into the pGEM-T easy vector for sequencing. The complete sequence of TRP19 gene was compared to those in the GenBank, revealing high identicalness. Phylogenetic analysis on the TRP36 gene sequences demonstrated two distinct strains from two dogs, named 56C and 70C. The 56C strain was grouped with the strain Cuiaba 16, which is a hybrid strain formed by Brazilian and US genogroups; and the 70C strain was grouped with other strains of the US genogroup, thus suggesting that there are at least two genogroups of E. canis in Rio de Janeiro (US and Brazilian). Those animals, in which the 70C and 56C strains were isolated, showed distinct clinical and hematological manifestations of 1the disease. The appearance of different genotypes may express new phenotypes, thus resulting in different forms of presentation of the disease and making its diagnosis more complex.

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Section: Discussionsupporting

confidence: 83%

“…The phylogenetic analysis among the Brazilian and other worldwide E. canis strains was performed based on the model proposed by Aguiar et al (2013).…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil

Ferreira

Cerqueira

Castro

et al. 2014

Rev. Bras. Parasitol. Vet.

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“…The existence of cross-reactivity observed in the IFA among the species of the genus Ehrlichia (HARRUS & WANER, 2011) implies the possibility that an unrecognized ehrlichial agent is circulating among these animals. To date, only E. canis and an ehrlichial agent related to cattle and Rhipicephalus (Boophilus) microplus ticks have been isolated in Brazil (CABEZAS-CRUZ et al, 2012;AGUIAR et al, 2013AGUIAR et al, , 2014.…”

Section: Discussionmentioning

confidence: 99%

Serological evidence of exposure to tick-borne agents in opossums (Didelphis spp.) in the state of São Paulo, Brazil

Melo

Aguiar

Spolidorio

et al. 2016

Rev. Bras. Parasitol. Vet.

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Evidência sorológica de exposição a agentes transmitidos por carrapatos em gambás (Didelphis spp.) AbstractThis work involved a serological investigation of tick-borne pathogens in opossums in eight municipalities of the state of São Paulo, Brazil. Serum samples from 109 opossums (91 Didelphis aurita and 18 Didelphis albiventris) were tested to detect antibodies to Rickettsia rickettsii (Taiaçu strain, 1:64 cut-off) and Ehrlichia canis (São Paulo strain, 1:40 cut-off), by indirect immunofluorescence assay (IFA); and against Borrelia burgdorferi (strain G39/40) by enzyme-linked immunosorbent assay (ELISA). The presence of antibodies to anti-R. rickettsii, anti-E. canis and anti-B. burgdorferi was detected in 32 (29.35%), 16 (14.67%) and 30 (27.52%) opossums, respectively. Opossum endpoint titers ranged from 64 to 1,024 for R. rickettsii, from 40 to 160 for E. canis, and from 400 to >51,200 for B. burgdorferi. These serological results suggest that opossums have been exposed to Rickettsia spp., Ehrlichia spp., and B. burgdorferi-related agents in the state of São Paulo. Our study underscores the need for further research about these agents in this study area, in view of the occurrence of Spotted Fever and Baggio-Yoshinari Syndrome disease in humans in the state of São Paulo, Brazil.

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Epidemiological link between canine monocytic ehrlichiosis caused by Ehrlichia canis and the presence of Rhipicephalus sanguineus sensu stricto in Argentina

et al. 2021

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In this work, we analyze data that support an epidemiological link between cases of canine monocytic ehrlichiosis (CME) by Ehrlichia canis and the presence of Rhipicephalus sanguineus sensu stricto as vector in an endemic area for this tick in Argentina. In a blood sample of a 1-year-old toy poodle with CME compatible clinical signs, which showed CME typical morulae in monocytes in Giemsa-stained blood smear, DNA of E. canis was detected by PCR. Further, DNA of E. canis was also detected in a female of R. sanguineus s.s. collected on the infected dog. Rhipicephalus sanguineus s.s. is the only member of the R. sanguineus group that prevails in the study area. The results of this study suggest that R. sanguineus s.s. may play a more important role in the transmission of E. canis than it was assumed so far. The epidemiological link between CME cases and R. sanguineus s.s. as vector in temperate areas of Argentina described in this work contrast previous studies which found that R. sanguineus sensu lato "tropical lineage" (which is absent in the study area) is competent to transmit E. canis but not R. sanguineus s.s.

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Genetic diversity of Ehrlichia canis in Brazil

Cited by 54 publications

References 13 publications

Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil

Genetic diversity of Ehrlichia canisstrains from naturally infected dogs in Rio de Janeiro, Brazil

Serological evidence of exposure to tick-borne agents in opossums (Didelphis spp.) in the state of São Paulo, Brazil

Epidemiological link between canine monocytic ehrlichiosis caused by Ehrlichia canis and the presence of Rhipicephalus sanguineus sensu stricto in Argentina

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