Genetic diversity in a collection of European pear (Pyrus communis) cultivars determined with SSR markers chosen by ECPGR

Sehic, Jasna; Garkava-Gustavsson, Larisa; Fernández-Fernández, Felicidad; Nybom, Hilde

doi:10.1016/j.scienta.2012.07.023

Cited by 52 publications

(40 citation statements)

References 24 publications

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“…S-genotyping of 'Ingeborg' revealed that this cultivar possesses the following three S alleles: S102, S108, and S121, labeled in accordance with the numeration proposed by Goldway et al (2009). Considering 'Ingeborg', parent cultivar 'Conference' possesses S108 and S121 S alleles (Sanzol, 2009), whereas the other parent 'Bonne Louise' possesses S101 and S102 S alleles (Sanzol and Robbins, 2008), and the obtained S allele profile confirms the conclusion by Sehic et al (2012) that 'Ingeborg' is derived from an unreduced 'Conference' egg cell being fertilized by a haploid pollen cell from 'Bonne Louise'. The results of S-genotyping obtained for the five pollinizer cultivars revealed that only 'Anna' possesses an S allele already present in 'Ingeborg' (S108), making it a semicompatible pollinizer (manuscript is in press).…”

Section: Resultssupporting

confidence: 79%

“…'Ingeborg' is a triploid (3x) and is believed to be the result of fertilization of an unreduced diploid (2n) egg cell from 'Conference' with a haploid (n) pollen cell from 'Bonne Louise' (Sehic et al, 2012). Although 'Ingeborg' possesses good pomological traits that are highly desirable to Norwegians, fruit set and subsequent yields of this cultivar tend to be erratic and significantly lower than other pear varieties grown in Norway (Meland and Frøynes, 2014).…”

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confidence: 99%

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Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Gaši¹,

Pojskić²,

Kurtović³

et al. 2017

horts

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Section: Resultssupporting

confidence: 79%

mentioning

confidence: 99%

Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Gaši¹,

Pojskić²,

Kurtović³

et al. 2017

horts

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“…Additionally, a large number of SSRs isolated from apple have revealed transferability to pear [11,12]. These markers have been used for Pyrus genome mapping [7,[12][13][14][15], as well as for genetic resource characterization [16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of wild pear (Pyrus pyraster (L.) Burgsd.) in Poland

et al. 2014

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In order to provide molecular characteristics of wild pear (P. pyraster) resources, six populations (192 accessions) from different regions of Poland were investigated with 17 SSR loci. Each of the SSR loci used was polymorphic, with a mean of 19.5 alleles per locus and a mean PIC of 0.806. Both the high heterozygosity (Ho = 0.751) and low Fis (0.007) indicated that the wild pear populations maintain a relatively high level of diversity, while the mean Findex of 0.039 and the number of migrants per generation (Nm = 6.996) revealed a high gene flow and weak inter-population differentiation. AMOVA analysis located polymorphisms mainly within populations (96%). Genetic relations between populations did not show correlations with geographical distances. The dispersal influence of gene flow could be the reason of the disrupted relationship within populations and the low interpopulation differentiation. We did not find any evidence to support the hypothesis about influence of interspecies hybridization with pear cultivars on the level of wild pear population diversity.

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“…Since both isolates were obtained from the same strain collection, a mislabeling cannot be excluded. DNA fingerprinting techniques are frequently used to verify strain and germplasm collections and successfully discover mislabeling or identify duplicates [48]. Pairwise genetic distances within the T. virens set ranged from 0.18 to 0.27 (Table 3), with the least genetic distance between the isolates from the USA and Australia (0.18).…”

Section: Discussionmentioning

confidence: 99%

SSR Markers for Trichoderma virens: Their Evaluation and Application to Identify and Quantify Root-Endophytic Strains

et al. 2015

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Using biological fertilizers and pesticides based on beneficial soil microbes in order to reduce mineral fertilizers and chemical pesticides in conventional agriculture is still a matter of debate. In this regard, a European research project seeks to elucidate the role of root-endophytic fungi and to develop molecular tools to trace and quantify these fungi in the rhizosphere and root tissue. To do this, the draft genome sequence of the biocontrol fungus Trichoderma virens (T. virens) was screened for simple sequence repeats (SSRs) and primers were developed for 12 distinct loci. Primers were evaluated using a global collection of ten isolates where an average of 7.42 alleles per locus was detected. Nei's standard genetic distance ranged from 0.18 to 0.27 among the isolates, and the grand mean of haploid diversity in AMOVA analysis was 0.693 ± 0.019. Roots of tomato plants were inoculated with different strains and harvested six weeks later. Subsequent PCR amplification identified root-endophytic strains and co-colonization of roots by different strains. Markers were applied to qPCR to quantify T. virens strains in root tissue and to determine their identity using allele-specific melting curve analysis. Thus, the root-endophytic lifestyle of T. virens was OPEN ACCESSDiversity 2015, 7 361 confirmed, strains in roots were quantified and simultaneous colonization of roots by different strains was observed.

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Genetic diversity in a collection of European pear (Pyrus communis) cultivars determined with SSR markers chosen by ECPGR

Cited by 52 publications

References 24 publications

Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Genetic diversity and population structure of wild pear (Pyrus pyraster (L.) Burgsd.) in Poland

SSR Markers for Trichoderma virens: Their Evaluation and Application to Identify and Quantify Root-Endophytic Strains

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