Genetic diversity and population structure of Tunisian wild Kermes oak (Quercus coccifera L.): Assessment by ISSR molecular markers and implication for conservation

Genetic variation of Amaranthus retroflexus L. and Chenopodium album L. (Amaranthaceae) suggests multiple independent introductions into Iran

“…Moreover based on PIC values, it can be concluded that the capacity of the marker system to detect polymorphic loci in a single amplification was very efficient; the average value of this coefficient amounted 0.78 for A. retroflexus L. and 0.71 for C. album L.. These results demonstrate this technique can be conveniently used for the genetic characterization of these populations of A. retroflexus L. and C. album L. Use of ISSR markers are also recently reported as a functional markers elsewhere (Sivaprakash et al, 2004;Yan et al, 2019;Kwiecińska-Poppe et al, 2020;Alotaibi and Abd-Elgawad, 2022;Flihi et al, 2022;Ghanbari et al, 2022;Haq et al, 2022).…”

“…ISSR markers are highly reproducible and accurate tools that generate highly reproducible banding patterns from a single polymerase chain reaction (PCR) amplification (Raut et al, 2014;Stefunova et al, 2014). Although newer technique are available, ISRR makers have historically (Wolfe et al, 1998) and recently (Yan et al, 2019;Kwiecińska-Poppe et al, 2020;Tang and Ma, 2020;Liu et al, 2021;Alotaibi and Abd-Elgawad, 2022;Flihi et al, 2022;Ghanbari et al, 2022;Haq et al, 2022) been used successfully for diversity studies and structuring of natural populations. Genomic DNA was isolated from the young leaves of plants according to the cetyltrimethylammonium bromide (CTAB) method described by Saghai-Maroof et al (1984).…”