“…During the past 20 years, different molecular tecniques have been used in trees, such as simple sequence repeats or microsatellites (SSR), chloroplast microsatellites (cpSSR), random amplified polymorphic DNA (RAPD), amplified fragment length polymorphisms (AFLP), polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP), and sequence variation in both mitochondrial DNA (mtDNA), and chloroplast DNA (cpDNA) regions (Wehenkel et al 2017). Different genetic diversity parameters have been usually calculated, such as the expected heterozygosity (He), the percentage of polymorphic loci (% P), the Shannon diversity index (S), the total haplotype diversity (H), the nucleotide diversity (π), the observed degrees of Gregorius' total differentiation (δT), and mean genetic diversity (vmean,2) among many others (Wehenkel et al 2017).…”